LOCUS       sequence-456551-       12134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12134)
  TITLE     NFAT plasmids
REFERENCE   2  (bases 1 to 12134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pREP-NFAT4
FEATURES             Location/Qualifiers
     source          1..12134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..262
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     306..322
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     306..322
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3090..3106)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3091..3107)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3122..3256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3146..3165)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3200..3219
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3399..3421)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3981..5770
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(7813..7831)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7899..8003
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8004..8072,8073..8864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8222..8241)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9035..9623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9524..9543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10054..10199
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10242..11279
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11277..11296)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11321..11369
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(11839..11858)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc
      241 attcaccaca ttggtgtgca cctccaagct gggtaccagc tgctagcaag cttgctagcg
      301 gccgctctag aactagtgga tcccccgggc tgcaggaatt cggcacgagc ggctgcggtt
      361 cctggtgctg ctcggcgcgc ggccagcttt cggaacggaa cgctcggcgt cgcgggcccc
      421 gcccggaaag tttgccgtgg agtcgcgacc tcttggcccg cgcggcccgg catgaagcgg
      481 cgttgaggag ctgctgccgc cgcttgccgc tgccgccgcc gccgcctgag gaggagctgc
      541 agcaccctgg gccacgccga tgactactgc aaactgtggc gcccacgacg agctcgactt
      601 caaactcgtc tttggcgagg acggggcgcc ggcgccgccg cccccgggct cgcggcctgc
      661 agatcttgag ccagatgatt gtgcatccat ttacatcttt aatgtagatc cacctccatc
      721 tactttaacc acaccacttt gcttaccaca tcatggatta ccgtctcact cttctgtttt
      781 gtcaccatcg tttcagctcc aaagtcacaa aaactatgaa ggaacttgtg agattcctga
      841 atctaaatat agcccattag gtggtcccaa accctttgag tgcccaagta ttcaaattac
      901 atctatctct cctaactgtc atcaagaatt agatgcacat gaagatgacc tacagataaa
      961 tgacccagaa cgggaatttt tggaaaggcc ttctagagat catctctatc ttcctcttga
     1021 gccatcctac cgggagtctt ctcttagtcc tagtcctgcc agcagcatct cttctaggag
     1081 ttggttctct gatgcatctt cttgtgaatc gctttcacat atttatgatg atgtggactc
     1141 agagttgaat gaagctgcag cccgatttac ccttggatcc cctctgactt ctcctggtgg
     1201 ctctccaggg ggctgccctg gagaagaaac ttggcatcaa cagtatggac ttggacactc
     1261 attatcaccc aggcaatctc cttgccactc tcctagatcc agtgtcactg atgagaattg
     1321 gctgagcccc aggccagcct caggaccctc atcaaggccc acatccccct gtgggaaacg
     1381 gaggcactcc agtgctgaag tttgttatgc tgggtccctt tcaccccatc actcacctgt
     1441 tccttcacct ggtcactccc ccaggggaag tgtgacagaa gatacgtggc tcaatgcttc
     1501 tgtccatggt gggtcaggcc ttggccctgc agtttttcca tttcagtact gtgtagagac
     1561 tgacatccct ctcaaaacaa ggaaaacttc tgaagatcaa gctgccatac taccaggaaa
     1621 attagagctg tgttcagatg accaagggag tttatcacca gcccgggaga cttcaataga
     1681 tgatggcctt ggatctcagt atcctttaaa gaaagattca tgtggtgatc agtttctttc
     1741 agttccttca ccctttacct ggagcaaacc aaagcctggc cacaccccta tatttcgcac
     1801 atcttcatta cctccactag actggccttt accagctcat tttggacaat gtgaactgaa
     1861 aatagaagtg caacctaaaa ctcatcatcg agcccattat gaaactgaag gtagccgagg
     1921 ggcagtaaaa gcatctactg ggggacatcc tgttgtgaag ctcctgggct ataacgaaaa
     1981 gccaataaat ctacaaatgt ttattgggac agcagatgat cgatatttac gacctcatgc
     2041 attttaccag gtgcatcgaa tcactgggaa gacagtcgct actgcaagcc aagagataat
     2101 aattgccagt acaaaagttc tggaaattcc acttcttcct gaaaataata tgtcagccag
     2161 tattgattgt gcaggtattt tgaaactccg caattcagat atagaacttc gaaaaggaga
     2221 aactgatatt ggcagaaaga atactagagt acgacttgtg tttcgtgtac acatcccaca
     2281 gcccagtgga aaagtccttt ctctgcagat agcctctata cccgttgagt gctcccagcg
     2341 gtctgctcaa gaacttcctc atattgagaa gtacagtatc aacagttgtt ctgtaaatgg
     2401 aggtcatgaa atggttgtga ctggatctaa ttttcttcca gaatccaaaa tcatttttct
     2461 tgaaaaagga caagatggac gacctcagtg ggaggtagaa gggaagataa tcagggaaaa
     2521 atgtcaaggg gctcacattg tccttgaagt tcctccatat cataacccag cagttacagc
     2581 tgcagtgcag gtgcactttt atctttgcaa tggcaagagg aaaaaaagcc agtctcaacg
     2641 ttttacttat acaccaggta cgaggagtca tgatggttta ctatagagct ttctttccta
     2701 atgaataaaa agttatttaa cgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2761 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2821 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2881 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2941 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3001 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3061 actcgtgccg aattcgatat caagcttatc gataccgtcg acctcgaggc cggcaaggcc
     3121 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3181 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3241 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3301 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     3361 tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     3421 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     3481 tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt cgactctaga ggatcgatcc
     3541 ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc
     3601 atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca
     3661 cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat
     3721 gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac
     3781 tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg
     3841 ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga
     3901 ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata
     3961 aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat
     4021 ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt
     4081 agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt
     4141 tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag
     4201 tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc
     4261 tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg
     4321 ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg
     4381 tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt
     4441 gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc
     4501 gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt
     4561 gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca
     4621 tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc
     4681 cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata
     4741 aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc
     4801 ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt
     4861 aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg
     4921 gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag
     4981 gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca
     5041 cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata
     5101 tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     5161 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     5221 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     5281 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga
     5341 ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg
     5401 gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5461 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5521 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     5581 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     5641 ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt agtagagtgg
     5701 gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt cgctgcttgt
     5761 ccttttcctg ctggttgctc ccattcttag gtgaatttaa ggaggccagg ctaaagccgt
     5821 cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg agaaggtgtt
     5881 gagcgcggag ctgagtgacg tgacaacatg ggtatgccca attgccccat gttgggagga
     5941 cgaaaatggt gacaagacag atggccagaa atacaccaac agcacgcatg atgtctactg
     6001 gggatttatt ctttagtgcg ggggaataca cggcttttaa tacgattgag ggcgtctcct
     6061 aacaagttac atcactcctg cccttcctca ccctcatctc catcacctcc ttcatctccg
     6121 tcatctccgt catcaccctc cgcggcagcc ccttccacca taggtggaaa ccagggaggc
     6181 aaatctactc catcgtcaaa gctgcacaca gtcaccctga tattgcaggt aggagcgggc
     6241 tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat atgagtttgt
     6301 aaaaagacca tgaaataaca gacaatggac tcccttagcg ggccaggttg tgggccgggt
     6361 ccaggggcca ttccaaaggg gagacgactc aatggtgtaa gacgacattg tggaatagca
     6421 agggcagttc ctcgccttag gttgtaaagg gaggtcttac tacctccata tacgaacaca
     6481 ccggcgaccc aagttccttc gtcggtagtc ctttctacgt gactcctagc caggagagct
     6541 cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc acgatgcttt
     6601 ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct gaccccgggg tccagtgctt
     6661 gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg cacgtcaggc
     6721 tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc tacagggtgg
     6781 aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga tgatgactga
     6841 ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct ggctctttca
     6901 cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact acctcctcga
     6961 ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc ccggcctcca
     7021 cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg ctcctctcct gcccctcctg
     7081 ctcctgcccc tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa
     7141 tgcaacttgg acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg
     7201 agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc
     7261 catggttatc accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg
     7321 tgtctccctt ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga
     7381 ttcacactaa aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag
     7441 tgcagactcc tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca
     7501 gacagatcca ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt
     7561 actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc
     7621 caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac
     7681 ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag
     7741 ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt
     7801 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     7861 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7921 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7981 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8041 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8101 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8161 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8221 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     8281 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8341 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8401 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8461 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8521 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     8581 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8641 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8701 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8761 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8821 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8881 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8941 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9181 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9661 tttgctggcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     9721 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     9781 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg
     9841 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     9901 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     9961 gaggtgccgc cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
    10021 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
    10081 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg
    10141 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac
    10201 agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca
    10261 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
    10321 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
    10381 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
    10441 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
    10501 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
    10561 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
    10621 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
    10681 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
    10741 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
    10801 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
    10861 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
    10921 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
    10981 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
    11041 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
    11101 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
    11161 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
    11221 atagtggaaa ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa
    11281 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
    11341 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
    11401 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
    11461 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
    11521 aggccctgcc atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt
    11581 ttatggttcg tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact
    11641 gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac
    11701 acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga
    11761 tttctggcgt gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
    11821 cgtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
    11881 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
    11941 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
    12001 tcgcgatgta cgggccagat atacgcgtat ctgaggggac tagggtgtgt ttaggcgaaa
    12061 agcggggctt cggttgtacg cggttaggag tcccctcagg atatagtagt ttcgcttttg
    12121 catagggagg ggga
//
