LOCUS       sequence-45656-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLKA-J72022
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    307..331
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             457..486
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(513..530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(592..616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        663..767
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata
       61 taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata
      121 taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc
      181 actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac acttaacggc
      241 tgacatggga attagccatg ggcccgtgcg aatcacggat ctcccgatca agaattgcca
      301 ttatcaacaa gtttgtacaa aaaagcaggc tccgaattgg tatcacgagg cagaatttca
      361 gataaaaaaa atccttagct ttcgctaagg atctgaagtg gaattcatga gatctacgca
      421 atatcacacg gaaccggagg gctaactcgc atgtctgaac aaaaactcat ctcagaagag
      481 gatctgggat cctaactcgc tcctcaggct tcctcgctca ctgactcgct gcgctcggtc
      541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatcaattc gacccagctt
      601 tcttgtacaa agtggttgat cggatctctt agacgtcagg tggcactttt cggggaaatg
      661 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      721 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      781 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      841 cagaaacgct ggtgaaagta aaagatgcag aggatcagtt gggtgcacga gtgggttaca
      901 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacg
//
