LOCUS       sequence-456575-       11713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Hoxa9 and MeisI to induce leukemic transformation. GFP label
            transduced cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11713)
  AUTHORS   He J, Nguyen AT, Zhang Y
  TITLE     KDM2b/JHDM1b, an H3K36me2-specific demethylase, is required for 
            initiation and maintenance of acute myeloid leukemia.
  JOURNAL   Blood. 2011 Apr 7;117(14):3869-80. doi: 
            10.1182/blood-2010-10-312736. Epub 2011 Feb 10.
  PUBMED    21310926
REFERENCE   2  (bases 1 to 11713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2010-10-312736"; journalName: "Blood"; date: 
            "2011-04-7- 7"; volume: "117"; issue: "14"; pages: "3869-80"
COMMENT     Sequence Label: pTY-EF1a-Hoxa9-p2a-MeisI-p2a-GFP
FEATURES             Location/Qualifiers
     source          1..11713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    220..444
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(577..596)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(1618..1635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1789..2377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1869..1888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2548..3339,3340..3408))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3171..3190
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3409..3513)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3581..3599
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3637..3659)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3759..3778
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5040..5060
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5103..5283
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6665..6898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7078..7195
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7573..8755
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7804..8746
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8703..8723
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             10894..11607
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             11617..11688
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggtacctt taagaccaat
       61 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
      121 gctaattcac tcccagggtc tctctggtta gaccagatct gagcctggga gctctctggc
      181 taactaggga acccactgct taagcctcat aaagctcgac tgtgccttct agttgccagc
      241 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      301 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
      361 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
      421 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
      481 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      541 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      601 ttctcgccac gttcgccggc tgtgcgggag aacggagttc tattatgact caaatcagtc
      661 tccccaagca ttcggggatc agagttttta aggataactt agtgtgtagg gggccagtga
      721 gttggagatg aaagcgtagg gagtcgaagg tgtccttttg cgccgagtca gttcctgggt
      781 gggggccaca agatcggatg agccagttta tcaatccggg ggtgccagct gatccatgga
      841 gtgcagggtc tgcaaaatat ctcaagcact gattgatctt aggttttaca atagtgatgt
      901 taccccagga acaatttggg gaaggtcaga atcttgtagc ctgtagctgc atgactccta
      961 aaccataatt tcttttttgt tttttttttt ttatttttga gacagggtct cactctgtca
     1021 cctaggctgg agtgcagtgg tgcaatcaca gctcactgca gcctcaacgt cgtaagctca
     1081 agcgatcctc ccacctcagc ctgcctggta gctgagacta caagcgacgc cccagttaat
     1141 ttttgtattt ttggtagagg cagcgttttg ccgtgtggcc ctggctggtc tcgaactcct
     1201 gggctcaagt gatccagcct cagcctccca aagtgctggg acaaccgggg ccagtcactg
     1261 cacctggccc taaaccataa tttctaatct tttggctaat ttgttagtcc tacaaaggca
     1321 gtctagtccc caggcaaaaa gggggtttgt ttcgggaaag ggctgttact gtctttgttt
     1381 caaactataa actaagttcc tcctaaactt agttcggcct acacccagga atgaacaagg
     1441 agagcttgga ggttagaagc acgatggaat tggttaggtc agatctcttt cactgtctga
     1501 gttataattt tgcaatggtg gttcaaagac tgcccgcttc tgacaccagt cgctgcatta
     1561 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1621 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1681 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1741 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1801 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1861 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1921 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1981 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2041 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2101 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2161 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2221 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2281 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2341 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2401 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2461 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2521 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2581 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2641 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2701 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2761 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2821 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2881 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2941 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3001 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3061 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3121 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3181 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3241 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3301 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3361 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3421 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3481 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3541 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     3601 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     3661 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     3721 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     3781 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     3841 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     3901 ttcgctatta cgccagggga ggcagagatt gcagtaagct gagatcgcag cactgcactc
     3961 cagcctgggc gacagagtaa gactctgtct caaaaataaa ataaataaat caatcagata
     4021 ttccaatctt ttcctttatt tatttattta ttttctattt tggaaacaca gtccttcctt
     4081 attccagaat tacacatata ttctattttt ctttatatgc tccagttttt tttagacctt
     4141 cacctgaaat gtgtgtatac aaaatctagg ccagtccagc agagcctaaa ggtaaaaaat
     4201 aaaataataa aaaataaata aaatctagct cactccttca catcaaaatg gagatacagc
     4261 tgttagcatt aaataccaaa taacccatct tgtcctcaat aattttaagc gcctctctcc
     4321 accacatcta actcctgtca aaggcatgtg ccccttccgg gcgctctgct gtgctgccaa
     4381 ccaactggca tgtggactct gcagggtccc taactgccaa gccccacagt gtgccctgag
     4441 gctgcccctt ccttctagcg gctgccccca ctcggctttg ctttccctag tttcagttac
     4501 ttgcgttcag ccaaggtctg aaactaggtg cgcacagagc ggtaagactg cgagagaaag
     4561 agaccagctt tacagggggt ttatcacagt gcaccctgac agtcgtcagc ctcacagggg
     4621 gtttatcaca ttgcaccctg acagtcgtca gcctcacagg gggtttatca cagtgcaccc
     4681 ttacaatcat tccatttgat tcacaatttt tttagtctct actgtgccta acttgtaagt
     4741 taaatttgat cagaggtgtg ttcccagagg ggaaaacagt atatacaggg ttcagtacta
     4801 tcgcatttca ggcccgagag ctgcatccgg agtatctaga tggagttccg cgttacataa
     4861 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     4921 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggagttt
     4981 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
     5041 gcaaatgggc ggtaggcgtg tactctagaa ggtctatata agcagagctc gtttagtgaa
     5101 ccgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5161 cactgcttaa gcctcaataa agcttgcctt gagtgctcaa agtagtgtgt gcccgtctgt
     5221 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5281 gcagtggcgc ccgaacaggg acttgaaagc gaaagtaaag ccagaggaga tctctcgacg
     5341 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tgcagagtac
     5401 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcggtat
     5461 taagcggggg agaattagat aaatgggaaa aaattcggtt aaggccaggg ggaaagaaac
     5521 aatataaact aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     5581 ctggcctttt agagacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     5641 ttcagacagg atcagaagaa cttagatcat tatataatac aatagcagtc ctctattgtg
     5701 tgcatcaaag gatagatgta aaagacacca aggaagcctt agataagata gaggaagagc
     5761 aaaacaaaag taagaaaaag gcacagcaag cagcagctga cacaggaaac aacagccagg
     5821 tcagccaaaa ttaccctata gtgcagaacc tccaggggca aatggtacat caggccatat
     5881 cacctagaac tttaaatgca tgggtaaaag tagtagaaga gaaggctttc agcccagaag
     5941 taatacccat gttttcagca ttatcagaag gagccacccc acaagattta aataccatgc
     6001 taaacacagt ggggggacat caagcagcca tgcaaatgtt aaaagagacc atcaatgagg
     6061 aagctgcaga atgggataga ttgcatccag tgcatgcagg gcctattgca ccaggccaga
     6121 tgagagaacc aaggggaagt gacatagcag gaactactag caaattaaga gaacaatttg
     6181 gaaataataa aacaataatc tttaagcaat cctcaggagg ggacccagaa attgtaacgc
     6241 acagttttaa ttgtggaggg gaatttttct actgtaattc aacacaactg tttaatagta
     6301 cttggtttaa tagtacttgg agtactgaag ggtcaaataa cactgaagga agtgacacaa
     6361 tcacactccc atgcagaata aaacaattta taaacatgtg gcaggaagta ggaaaagcaa
     6421 tgtatgcccc tcccatcagt ggacaaatta gatgttcatc aaatattact gggctgctat
     6481 taacaagaga tggtggtaat aacaacaatg ggtccgagat cttcagacct ggaggaggcg
     6541 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     6601 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     6661 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     6721 caatgacgct gacggtacag gccagacaat tattgtctga tatagtgcag cagcagaaca
     6781 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     6841 aacagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     6901 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     6961 ggagtaataa atctctggaa cagatttgga ataacatgac ctggatggag tgggacagag
     7021 aaattaacaa ttacacaagc ttaagacagc agtacaaatg gcagtattca tccacaattt
     7081 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     7141 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     7201 ttattacagg gacagcagag atccagttgc ggccaattca gattaaggcc gtggccataa
     7261 tcacgaattg gccgcagatc tcgaccaatt ctcatgtttg acagcttatc atcgataagc
     7321 tttggagcta agccagcaat ggtagaggga agattctgca cgtcccttcc aggcggcctc
     7381 cccgtcacca ccccccccaa cccgccccga ccggagctga gagtaattca tacaaaagga
     7441 ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa ctcccactaa cgtagaaccc
     7501 agagatcgct gcgttcccgc cccctcaccc gcccgctctc gtcatcactg aggtggagaa
     7561 gagcatgcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     7621 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaagtg gcgcggggta
     7681 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     7741 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     7801 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     7861 gtgccttgaa ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg
     7921 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     7981 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     8041 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     8101 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     8161 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     8221 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     8281 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     8341 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     8401 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     8461 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     8521 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     8581 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     8641 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     8701 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaaaact
     8761 ctagcgtttc cgctctagaa ctagtccacc atggccacca ccggggccct gggcaactac
     8821 tatgtggact ccttcctgct gggcgccgac gctgctgatg agctgggtgc gggacgctac
     8881 gctccaggga ccctgggtca acccccaagg caggcggcag ctctggccga acaccccgac
     8941 ttcagtcctt gcagcttcca gtccaaggcg gcggtgtttg gtgcctcgtg gaacccagtg
     9001 cacgcggcgg gcgccaatgc ggtgcctgct gcagtgtatc atcaccacca ccacccctac
     9061 gtgcatcccc aggcgcccgt ggcggcggcg gcgccggacg gcaggtatat gcgctcctgg
     9121 ctggaaccca cgcccggtgc gctctccttc gcgggcttac cctccagccg gccttatggc
     9181 attaaacctg aaccgctctc ggccagaagg ggtgactgtc ccacgcttga cactcacact
     9241 ttgtccctga ctgactatgc ttgtggttct cctccagttg atagagaaaa acaacccagc
     9301 gaaggcgcct tctccgaaaa caatgccgag aatgagagcg gcggagacaa gccccccatc
     9361 gatcccaata acccggctgc caactggcta catgctcgct ccactcggaa gaagcgatgc
     9421 ccctacacaa aacaccagac gctggaactg gagaaggagt ttctgtttaa catgtacctc
     9481 acacgggacc gcaggtacga ggtggcccgg ctgctcaacc tcaccgaaag gcaggtcaag
     9541 atctggttcc agaaccgcag gatgaaaatg aagaaaatca acaaggaccg agcaaaagac
     9601 gagcaactgc tgaattttga ccttcttaag ttggcgggag acgtcgagtc caaccctggg
     9661 cccatggcgc aaaggtacga cgacctaccc cattatgggg gtatggatgg agtaggcatc
     9721 ccctccacga tgtatgggga cccgcatgca gccaggtcca tgcaaccggt ccaccacctg
     9781 aaccacgggc ctcctctgca ctcgcatcag tacccgcaca cagctcacac caacgccatg
     9841 gcccccagca tgggttcctc ggtcaatgac gctttaaaga gagataaaga tgccatttat
     9901 ggacaccccc tcttccctct cttagcactg atttttgaga aatgtgaatt agctacttgt
     9961 accccccgcg agccgggggt ggcgggcggg gacgtctgct cgtcagagtc attcaatgaa
    10021 gatatagcgg tgttcgccaa acagattcgc gcagaaaaac ctctattctc ttctaatcca
    10081 gaactggata acttgatgat tcaagccata caagtgttaa ggtttcatct gttggaatta
    10141 gagaaggtac acgaattatg tgacaatttc tgccaccggt atattagctg tttgaaaggg
    10201 aaaatgccta tcgatttggt gatagatgat agagaaggag gatcaaaatc agacagtgaa
    10261 gatgtaacaa gatcagcaaa tctaactgac cagccctctt ggaatagaga ccatgatgac
    10321 acggcatcca ctcgttcagg aggaaccccg ggcccttcca gcggtggcca tacttcacac
    10381 agtggggata acagcagtga gcaaggtgat ggcttggaca acagtgtagc ttcccccagc
    10441 acaggtgacg atgatgaccc tgataaggac aaaaagcgtc acaaaaagcg tggcatcttt
    10501 cccaaagtag ccaccaatat catgagggcg tggctgttcc agcatctaac acacccttac
    10561 ccttctgaag aacagaaaaa gcagttggca caagatacag gactcaccat ccttcaagtg
    10621 aacaattggt ttattaatgc ccggagaaga atagtgcagc ccatgataga ccagtccaac
    10681 cgagcagtca gccaagggac accttataac cctgatggac agccaatggg aggttttgta
    10741 atggacggtc agcagcacat gggcatcaga gcgccaggac ctatgagtgg aatgggcatg
    10801 aatatgggca tggaggggca gtggcactac atgcaactgc tgaattttga ccttcttaag
    10861 ttggcgggag acgtcgagtc caaccctggg cccatgagca agggcgagga actgttcact
    10921 ggcgtggtcc caattctcgt ggaactggat ggcgatgtga atgggcacaa attttctgtc
    10981 agcggagagg gtgaaggtga tgccacatac ggaaagctca ccctgaaatt catctgcacc
    11041 actggaaagc tccctgtgcc atggccaaca ctggtcacta ccctgaccta tggcgtgcag
    11101 tgcttttcca gatacccaga ccatatgaag cagcatgact ttttcaagag cgccatgccc
    11161 gagggctatg tgcaggagag aaccatcttt ttcaaagatg acgggaacta caagacccgc
    11221 gctgaagtca agttcgaagg tgacaccctg gtgaatagaa tcgagctgaa gggcattgac
    11281 tttaaggagg atggaaacat tctcggccac aagctggaat acaactataa ctcccacaat
    11341 gtgtacatca tggccgacaa gcaaaagaat ggcatcaagg tcaacttcaa gatcagacac
    11401 aacattgagg atggatccgt gcagctggcc gaccattatc aacagaacac tccaatcggc
    11461 gacggccctg tgctcctccc agacaaccat tacctgtcca cccagtctgc cctgtctaaa
    11521 gatcccaacg aaaagagaga ccacatggtc ctgctggagt ttgtgaccgc tgctgggatc
    11581 acacatggca tggacgagct gtacaagtga gaattcgatt acaaggatga cgacgataag
    11641 gactataagg acgatgatga caaggactac aaagatgatg acgataaata gctcgaaatt
    11701 cgataattcg ccc
//
