LOCUS       sequence-456577-       10403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Martinez-Noel G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper 
            JW, Howley PM
  TITLE     Identification and proteomic analysis of distinct UBE3A/E6AP protein
            complexes.
  JOURNAL   Mol Cell Biol. 2012 May 29.
  PUBMED    22645313
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2012 May 29."
COMMENT     Sequence Label: p6895 PHAGE-P CMVT N-HA-hE6AP I wt
FEATURES             Location/Qualifiers
     source          1..10403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..169
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..699
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    730..748
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    751..769
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     779..803
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      890..899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             902..928
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    944..968
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3570..3594)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3613..4100
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3648..3671)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4033..4052
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4121..4720
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4121..4140)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4617..4637
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4787..5375
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4840..4860)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5258..5269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5450..5683
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5802..5820)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5888..5992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5993..6061,6062..6853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6211..6230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7024..7612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7513..7532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7766..7783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8023..8044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8059..8064,8065..8082,8083..8089)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8097..8113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8102..8124
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8121..8137
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8121..8137
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8187..8206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8398..9031
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9078..9203
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9700..9933
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10118..10162
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acggatctcg acggtatcgc cgaattcaca aatggcagta ttcatccaca attttaaaag
       61 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      121 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
      181 cagggacagc agagatccag tttggactag tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      661 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      721 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
      781 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      841 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacgctag ccaccatggc
      901 gtacccatac gatgttccag attacgctgc tcgaggttat caaacaagtt tgtacaaaaa
      961 agcaggctcc gcggccgccc ccttcaccat gaagcgagca gctgcaaagc atctaataga
     1021 acgctactac caccagttaa ctgagggctg tggaaatgaa gcctgcacga atgagttttg
     1081 tgcttcctgt ccaacttttc ttcgtatgga taataatgca gcagctatta aagccctcga
     1141 gctttataag attaatgcaa aactctgtga tcctcatccc tccaagaaag gagcaagctc
     1201 agcttacctt gagaactcga aaggtgcccc caacaactcc tgctctgaga taaaaatgaa
     1261 caagaaaggc gctagaattg attttaaaga tgtgacttac ttaacagaag agaaggtata
     1321 tgaaattctt gaattatgta gagaaagaga ggattattcc cctttaatcc gtgttattgg
     1381 aagagttttt tctagtgctg aggcattggt acagagcttc cggaaagtta aacaacacac
     1441 caaggaagaa ctgaaatctc ttcaagcaaa agatgaagac aaagatgaag atgaaaagga
     1501 aaaagctgca tgttctgctg ctgctatgga agaagactca gaagcatctt cctcaaggat
     1561 aggtgatagc tcacagggag acaacaattt gcaaaaatta ggccctgatg atgtgtctgt
     1621 ggatattgat gccattagaa gggtctacac cagattgctc tctaatgaaa aaattgaaac
     1681 tgcctttctc aatgcacttg tatatttgtc acctaacgtg gaatgtgact tgacgtatca
     1741 caatgtatac tctcgagatc ctaattatct gaatttgttc attatcgtaa tggagaatag
     1801 aaatctccac agtcctgaat atctggaaat ggctttgcca ttattttgca aagcgatgag
     1861 caagctaccc cttgcagccc aaggaaaact gatcagactg tggtctaaat acaatgcaga
     1921 ccagattcgg agaatgatgg agacatttca gcaacttatt acttataaag tcataagcaa
     1981 tgaatttaac agtcgaaatc tagtgaatga tgatgatgcc attgttgctg cttcgaagtg
     2041 cttgaaaatg gtttactatg caaatgtagt gggaggggaa gtggacacaa atcacaatga
     2101 agaagatgat gaagagccca tccctgagtc cagcgagctg acacttcagg aacttttggg
     2161 agaagaaaga agaaacaaga aaggtcctcg agtggacccc ctggaaactg aacttggtgt
     2221 taaaaccctg gattgtcgaa aaccacttat cccttttgaa gagtttatta atgaaccact
     2281 gaatgaggtt ctagaaatgg ataaagatta tacttttttc aaagtagaaa cagagaacaa
     2341 attctctttt atgacatgtc cctttatatt gaatgctgtc acaaagaatt tgggattata
     2401 ttatgacaat agaattcgca tgtacagtga acgaagaatc actgttctct acagcttagt
     2461 tcaaggacag cagttgaatc catatttgag actcaaagtt agacgtgacc atatcataga
     2521 tgatgcactt gtccggctag agatgatcgc tatggaaaat cctgcagact tgaagaagca
     2581 gttgtatgtg gaatttgaag gagaacaagg agttgatgag ggaggtgttt ccaaagaatt
     2641 ttttcagctg gttgtggagg aaatcttcaa tccagatatt ggtatgttca catacgatga
     2701 atctacaaaa ttgttttggt ttaatccatc ttcttttgaa actgagggtc agtttactct
     2761 gattggcata gtactgggtc tggctattta caataactgt atactggatg tacattttcc
     2821 catggttgtc tacaggaagc taatggggaa aaaaggaact tttcgtgact tgggagactc
     2881 tcacccagtt ctatatcaga gtttaaaaga tttattggag tatgaaggga atgtggaaga
     2941 tgacatgatg atcactttcc agatatcaca gacagatctt tttggtaacc caatgatgta
     3001 tgatctaaag gaaaatggtg ataaaattcc aattacaaat gaaaacagga aggaatttgt
     3061 caatctttat tctgactaca ttctcaataa atcagtagaa aaacagttca aggcttttcg
     3121 gagaggtttt catatggtga ccaatgaatc tcccttaaag tacttattca gaccagaaga
     3181 aattgaattg cttatatgtg gaagccggaa tctagatttc caagcactag aagaaactac
     3241 agaatatgac ggtggctata ccagggactc tgttctgatt agggagttct gggaaatcgt
     3301 tcattcattt acagatgaac agaaaagact cttcttgcag tttacaacgg gcacagacag
     3361 agcacctgtg ggaggactag gaaaattaaa gatgattata gccaaaaatg gcccagacac
     3421 agaaaggtta cctacatctc atacttgctt taatgtgctt ttacttccgg aatactcaag
     3481 caaagaaaaa cttaaagaga gattgttgaa ggccatcacg tatgccaaag gatttggcat
     3541 gctgtaagga tccaagggtg ggcgcgccga cccagctttc ttgtacaaag tggttcgata
     3601 acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     3661 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     3721 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     3781 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     3841 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     3901 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     3961 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     4021 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     4081 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     4141 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     4201 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4261 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4321 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4381 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4441 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4501 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4561 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4621 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4681 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     4741 gcgcccgacc gaaaggagcg cacgacccca tgcatcgata gatcctaatc aacctctgga
     4801 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     4861 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4921 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4981 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5041 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5101 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5161 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     5221 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5281 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5341 gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact
     5401 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5461 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5521 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5581 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5641 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     5701 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg
     5761 ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt
     5821 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5881 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5941 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6001 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6061 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6121 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6181 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     6241 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6301 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6361 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6421 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6481 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6541 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6601 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6661 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6721 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6781 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6841 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7141 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7681 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7741 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7801 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc
     7861 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7921 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga
     7981 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8041 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8101 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa
     8161 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8221 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     8281 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     8341 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     8401 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     8461 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     8521 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     8581 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     8641 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     8701 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     8761 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     8821 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8881 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8941 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9001 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     9061 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     9121 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     9181 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     9241 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     9301 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     9361 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     9421 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     9481 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     9541 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9601 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9661 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9721 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9781 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9841 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9901 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9961 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    10021 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    10081 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    10141 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    10201 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    10261 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    10321 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
    10381 agacagatcc attcgattag tga
//
