LOCUS       sequence-456583-        9266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GRM6-Tango
FEATURES             Location/Qualifiers
     source          1..9266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3824..4438,4439..4825)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3891..3910)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4857..4874)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4863..5087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5133..5561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5220..5239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5430..5451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5570..5590)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5575..5904
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5755..5890
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5817..5836
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5971..6765
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6025..6044)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6635..6654
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6939..7060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6976..6995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7030..7049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7109..7125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7109..7125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7122..7144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7133..7149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7157..7163,7164..7181,7182..7187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7202..7223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7340..7357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7511..8099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7591..7610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8270..9061,9062..9130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8893..8912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9131..9235)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatccag cacagtggcg gccgcgccac catgaagacg atcatcgccc tgagctacat
     1021 cttctgcctg gtattcgccg actacaagga cgatgatgac gccagcatcg atatggctcg
     1081 gcctcggagg gcaagagaac ccctgctggt ggcccttttg cctctggctt ggttggcgca
     1141 agccggactc gccagggccg ctggaagcgt caggctggct ggaggcttga ccctcggagg
     1201 actgttcccc gtgcatgcaa gaggcgcagc tgggagggcc tgcggacagc tgaaaaagga
     1261 acagggagtg catagattgg aggcgatgct gtatgctctc gacagggtga atgccgaccc
     1321 cgaactgctg cccggggtgc ggctgggggc ccgactgctg gatacctgca gccgagatac
     1381 ttacgcactg gagcaagccc ttagcttcgt gcaggccctg atcagaggca gaggtgatgg
     1441 agacgaggtg ggagttagat gccccggcgg ggtgccccca cttagacctg ctccccccga
     1501 gagggtggtg gccgtggtgg gcgcttccgc ttcctctgtc tccattatgg tggcaaatgt
     1561 acttcgcctg tttgcaatcc cccaaatttc ctacgcatcc acggcccccg aattgtctga
     1621 ttctactagg tacgacttct tcagtcgagt ggtaccccct gatagctacc aggcacaggc
     1681 aatggtggat attgtcaggg cattggggtg gaactacgtg agtactctgg cctctgaggg
     1741 caactacggt gagtcagggg tagaggcctt cgtacaaatt tcacgggagg ccgggggagt
     1801 gtgcatcgcc cagtcaatca agataccacg agagcccaaa ccaggagagt tcagcaaagt
     1861 gattcggcgg ctgatggaaa cccctaatgc tcggggcatc atcatctttg ccaacgaaga
     1921 cgatatccga agggtgctgg aagcagctcg ccaagcgaac ctgaccggcc atttcctgtg
     1981 ggttggatcc gatagctggg gggcaaaaac aagtccaatc ttgtccttgg aggatgtagc
     2041 cgtcggggca attacaatcc tcccgaagcg agcaagtatt gatgggtttg atcagtactt
     2101 catgaccagg tcccttgaaa ataatcgccg caatatttgg ttcgcagagt tctgggaaga
     2161 aaattttaac tgcaagctga ccagtagcgg cacccaatct gatgacagta ccagaaaatg
     2221 caccggcgag gaacggattg gacgagattc tacctacgag caagagggca aggtccagtt
     2281 cgtgatcgac gccgtgtacg ctatagctca cgccctgcac agcatgcatc aggcactgtg
     2341 ccccggacac accggccttt gtccagccat ggagcccact gatgggcgga tgctcctgca
     2401 gtatattaga gctgtgcgat tcaacggctc cgcgggaacc ccagtgatgt tcaacgaaaa
     2461 tggggatgcc cctggaaggt acgatatctt tcagtatcag gccaccaatg gatccgcctc
     2521 cagcggggga tatcaggcgg tggggcaatg ggccgagacg ctccgcctcg atgtggaggc
     2581 attgcagtgg agtggcgatc cccacgaggt gcctagcagc ttgtgttcat tgccttgtgg
     2641 gccaggcgaa cgcaaaaaga tggtaaaagg ggtcccttgc tgctggcact gcgaagcttg
     2701 tgatgggtac agattccaag tggacgagtt tacatgcgaa gcatgtccag gcgacatgcg
     2761 cccgactcca aaccacaccg gctgtcggcc tacgcctgtg gttagactgt catggagttc
     2821 cccctgggca gctcctccac ttctgcttgc ggtattgggg attgtggcca cgacgacggt
     2881 ggttgcaact ttcgtccgct ataacaacac accgatcgtg agagcctccg gccgcgaact
     2941 gtcatacgtt ttgcttactg gaatattctt gatctacgct attacctttc tgatggtggc
     3001 cgaaccagga gccgccgtgt gtgccgctag acgactgttt ctcggcttgg gaaccacact
     3061 gagctactca gccctgctca ccaagactaa cagaatatac agaatcttcg aacagggaaa
     3121 gcgctcagta acaccaccac cctttatcag ccccactagc cagctggtca ttacttttag
     3181 tttgacctca ttgcaagtgg tcggaatgat tgcttggctg ggtgctagac caccgcacag
     3241 cgtcattgac tatgaagagc aacgaacagt ggatcccgag caggcgcggg gggtcctgaa
     3301 gtgtgacatg agcgaccttt ctctcatagg ttgcctcggc tatagcctgc tcttgatggt
     3361 cacttgcacc gtctatgcca tcaaggctag gggcgttcca gagacattca atgaagccaa
     3421 gcccattggc ttcaccatgt ataccacatg cattatttgg ctggcattcg ttccaatctt
     3481 ttttgggacc gcccagtccg ccgagaagat ctacatccag actaccactc tcaccgtgag
     3541 cttgtccctc agtgcctccg tatcacttgg gatgctctat gtgcccaaga cgtatgttat
     3601 tttgttccac ccagagcaga acgtgcagaa gagaaaacgc tccctgaaag ccacctcaac
     3661 ggtcgccgcg cctcctaaag gagaagatgc cgaggcccac aaaatcgata ccggtggacg
     3721 caccccaccc agcctgggtc cccaagatga gtcctgcacc accgccagct cctccctggc
     3781 caaggacact tcatcgaccg gtgagaacct gtacttccag ctaagattag ataaaagtaa
     3841 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
     3901 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
     3961 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
     4021 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
     4081 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
     4141 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
     4201 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
     4261 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
     4321 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
     4381 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
     4441 gtacagccgc gcgcgtacga aaaacaatta cgggtctacc atcgagggcc tgctcgatct
     4501 cccggacgac gacgcccccg aagaggcggg gctggcggct ccgcgcctgt cctttctccc
     4561 cgcgggacac acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc tgggggacga
     4621 gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga
     4681 tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc
     4741 cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct
     4801 tggaattgac gagtacggtg ggtagtctag agggcccgtt taaacccgct gatcagcctc
     4861 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4921 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4981 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5041 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5101 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5161 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5221 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5281 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5341 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5401 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5461 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5521 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5581 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5641 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5701 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5761 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5821 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5881 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5941 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6001 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6061 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6121 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6181 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6241 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6301 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6361 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6421 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6481 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6541 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6601 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6661 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6721 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6781 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     6841 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6901 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6961 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7021 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7081 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7141 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7201 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7321 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7921 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8161 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8221 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8281 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8341 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8401 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8461 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8521 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8581 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8641 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8701 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8761 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8821 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8881 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8941 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9001 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9061 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9121 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9241 ttccccgaaa agtgccacct gacgtc
//
