LOCUS       sequence-45659-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-K112603
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..37
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(67..84)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(146..170)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(320..339)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(404..423)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 attcatgaga tctgactaca aggatgacga cgacaagtaa ggatcctaac tcgacgtgca
       61 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      121 tcactcaaag gcggtaatca attcgaccca gctttcttgt acaaagtggt tgatcggatc
      181 tgctgatcct tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa
      241 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
      301 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc
      361 tccaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
      421 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
      481 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
      541 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
      601 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta
      661 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
      721 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
      781 caggcgcaat cacga
//
