LOCUS       sequence-456616-        9540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 9540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: FLAG-MARK3-GK-CR
FEATURES             Location/Qualifiers
     source          1..9540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2149..2172
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4372..4946,4947..4949,4950..4958)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4539..4556)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4766..4785
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    5397..5985
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5450..5470)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5868..5879)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6057..6290
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6368..6489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6405..6424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6459..6478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6529..6664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6684..6703)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6685..6703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6713..6730)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6713..6729)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6722..6744)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6871..7326
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6958..6977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7168..7189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7352..7456
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7457..7525,7526..8317)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7675..7694)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8488..9076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8977..8996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9230..9247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9364..9385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9400..9405,9406..9423,9424..9430)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9438..9454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9443..9465
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9462..9478
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9462..9478
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9497..9517
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9499..9517
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgga ttacaaagac
     2161 gatgacgata agatgtccac taggacccca ttgccaacgg tgaatgaacg agacactgaa
     2221 aaccacacgt cacatggaga tgggcgtcaa gaagttacct ctcgtaccag ccgctcagga
     2281 gctcggtgta gaaactctat agcctcctgt gcagatgaac aacctcacat cggaaactac
     2341 agactgttga aaacaatcgg caaggggaat tttgcaaaag taaaattggc aagacatatc
     2401 cttacaggca gagaggttgc aataaaaata attgacaaaa ctcagttgaa tccaacaagt
     2461 ctacaaaagc tcttcagaga agtaagaata atgaagattt taaatcatcc caatatagtg
     2521 aagttattcg aagtcattga aactgaaaaa acactctacc taatcggtga atatgcaagt
     2581 ggaggtgaag tatttgacta tttggttgca catggcagga tgaaggaaaa agaagcaaga
     2641 tctaaattta gacagattgt gtctgcagtt caatactgtc atcagaaacg gatcgtacat
     2701 cgagacctca aggctgaaaa tctattgtta gatgccgata tgaacattaa aatagcagat
     2761 ttcggtttta gcaatgaatt tactgttggc ggtaaactcg acacgttttg tggcagtcct
     2821 ccatacgcag cacctgagct cttccagggc aagaaatatg acgggccaga agtggatgtg
     2881 tggagtctgg gggtcatttt atacacacta gtcagtggct cacttccctt tgatgggcaa
     2941 aacctaaagg aactgagaga gagagtatta agagggaaat acagaattcc cttctacatg
     3001 tctacagact gtgaaaacct tctcaaacgt ttcctggtgc taaatccaat taaacgcggc
     3061 actctagagc aaatcatgaa ggacaggtgg atcaatgcag ggcatgaaga agatgaactc
     3121 aaaccatttg ttgaaccaga gctagacatc tcagaccaaa aaagaataga tattatggtg
     3181 ggaatgggat attcacaaga agaaattcaa gaatctctta gtaagatgaa atacgatgaa
     3241 atcacagcta catatttgtt attggggaga aaatcttcag agctggatgc tagtgattcc
     3301 agttctagca gcaatctttc acttgctaag gttaggccga gcagtgatct caacaacagt
     3361 actggccagt ctcctcacca caaagtgcag agaagtgttt cttcaagcca aaagcaaaga
     3421 cgctacagtg accatgctgg accagctatt ccttctgttg tggcgtatcc gaaaaggagt
     3481 cagaccagca ctgcagatag tgacctcaaa gaagatggaa tttcctcccg gaaatcaagt
     3541 ggcagtgctg ttggaggaaa gggaattgct ccagccagtc ccatgcttgg gaatgcaagt
     3601 aatcctaata aggcggatat tcctgaacgc aagaaaagct ccactgtccc tagtagtaac
     3661 acagcatctg gtggaatgac acgacgaaat acttatgttt gcagtgagag aactacagct
     3721 gatagacact cagtgattca gaatggcaaa gaaaacagca ctattcctga tcagagaact
     3781 ccagttgctt caacacacag tatcagtagt gcagccaccc cagatcgaat ccgcttccca
     3841 agaggcactg ccagtcgtag cactttccac ggccagcccc gggaacggcg aaccgcaaca
     3901 tataatggcc ctcctgcctc tcccagcctg tcccatgaag ccacaccatt gtcccagact
     3961 cgaagccgag gctccactaa tctctttagt aaattaactt caaaactcac aaggagtcgc
     4021 aatgtatctg ctgagcaaaa agatgaaaac aaagaagcaa agcctcgatc cctacgcttc
     4081 acctggagca tgaaaaccac tagttcaatg gatcccgggg acatgatgcg ggaaatccgc
     4141 aaagtgttgg acgccaataa ctgcgactat gagcagaggg agcgcttctt gctcttctgc
     4201 gtccacggag atgggcacgc ggagaacctc gtgcagtggg aaatggaagt gtgcaagctg
     4261 ccaagactgt ctctgaacgg ggtccggttt aagcggatat cggggacatc catagccttc
     4321 aaaaatattg cttccaaaat tgccaatgag ctaaagctgt gagcaattcc gcccctctcc
     4381 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     4441 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     4501 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     4561 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     4621 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     4681 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     4741 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     4801 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     4861 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     4921 tttcctttga aaaacacgat gataatatgg ccacaaccat gaagaccttc aacatctctc
     4981 agcaggacct tgagctggtg gaagttgcca ctgagaaaat caccatgctc tatgaggaca
     5041 acaagcacca tgttggtgct gccatcagga ccaagacagg agaaatcatc tctgctgtcc
     5101 acattgaagc ctacattggc agggtcactg tgtgtgcaga ggccattgcc attgggtctg
     5161 cagtctccaa tgggcagaag gactttgaca ccattgtggc tgtgaggcac ccctacagtg
     5221 atgaggtgga caggtccatc agagtggtgt ccccctgtgg catgtgcagg gagctcatct
     5281 cagactatgc ccctgattgc tttgttctga ttgagatgaa tggcaagctg gtcaagacaa
     5341 ccattgagga gctgattcca ctgaaataca ccagaaacta aacgcgttaa gtcgacaatc
     5401 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5461 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5521 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5581 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5641 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5701 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5761 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5821 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5881 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5941 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     6001 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6061 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6121 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6181 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6241 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     6301 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6361 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6421 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6481 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     6541 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6601 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6661 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     6721 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6781 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6841 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     6901 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6961 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7021 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7081 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7141 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7201 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7261 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     7321 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7381 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7441 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7501 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7561 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7621 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7681 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7741 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7801 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7861 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7921 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7981 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8041 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8101 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8161 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8221 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8281 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8341 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8401 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8461 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8521 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8581 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8641 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8701 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8761 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8821 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8881 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8941 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9001 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9061 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9241 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9301 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9361 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9421 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     9481 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
