LOCUS       sequence-456618-        9225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-MST1-7A
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3685..4259,4260..4262,4263..4271)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3852..3869)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4079..4098
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4272..5066
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4326..4345)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4936..4955
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5082..5670
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5135..5155)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5553..5564)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5742..5975
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6053..6174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6090..6109)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6144..6163
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6214..6349
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6369..6388)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6370..6388)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6398..6415)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6398..6414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6407..6429)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6556..7011
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6643..6662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6853..6874
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7037..7141
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7142..7210,7211..8002)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7360..7379)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8173..8761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8662..8681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8915..8932
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9049..9070
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9085..9090,9091..9108,9109..9115)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9123..9139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9128..9150
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9147..9163
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9147..9163
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9182..9202
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9184..9202
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccctacgac
     2161 gtgcccgact acgccggcta tccgtatgat gtcccggact atgcaggaag cggagagacg
     2221 gtacagctga ggaacccgcc gcgccggcag ctgaaaaagt tggatgaaga tagtttaacc
     2281 aaacaaccag aagaagtatt tgatgtctta gagaaacttg gagaagggtc ctatggcagc
     2341 gtatacaaag ctattcataa agagaccggc cagattgttg ctattaagca agttcctgtg
     2401 gaatcagacc tccaggagat aatcaaagaa atctctataa tgcagcaatg tgacagccct
     2461 catgtagtca aatattatgg cagttatttt aagaacacag acttatggat cgttatggag
     2521 tactgtgggg ctggttctgt atctgatatc attcgattac gaaataaaac gttaacagaa
     2581 gatgaaatag ctacaatatt acaatcaact cttaagggac ttgaatacct tcattttatg
     2641 agaaaaatac accgagatat caaggcagga aatattttgc taaatacaga aggacatgca
     2701 aaacttgcag attttggggt agcaggtcaa cttgcagata ccatggccaa gcggaataca
     2761 gtgataggaa caccattttg gatggctcca gaagtgattc aggaaattgg atacaactgt
     2821 gtagcagaca tctggtccct gggaataact gccatagaaa tggctgaagg aaagccccct
     2881 tatgctgata tccatccaat gagggcaatc ttcatgattc ctacaaatcc tcctcccaca
     2941 ttccgaaaac cagagctatg gtcagataac tttacagatt ttgtgaaaca gtgtcttgta
     3001 aagagccctg agcagagggc cacagccgca cagctcctgc agcacccatt tgtcaggagt
     3061 gccaaaggag tgtcaatact gcgagactta attaatgaag ccatggatgt gaaactgaaa
     3121 cgccaggaat cccagcagcg ggaagtggac caggacgatg aagaaaacgc agaagaggat
     3181 gaaatggatg caggcacgat ggttcgagca gtgggtgatg agatgggcgc agtccgagta
     3241 gccagcacca tgactgatgg agccaatact atgattgagc acgatgacac gttgccatca
     3301 caactgggca ccatggtgat caatgcagag gatgaggaag aggaaggaac tatgaaaaga
     3361 agggatgagg caatgcagcc tgcgaaacca tcctttcttg aatattttga acaaaaagaa
     3421 aaggaaaacc agatcaacag ctttggcaag agtgtacctg gtccactgaa aaattcttca
     3481 gattggaaaa taccacagga tggagactac gagtttctta agagttgggc agtggaggac
     3541 cttcagaaga ggctcttggc cctggacccc atgatggagc aggagattga agagatccgg
     3601 cagaagtacc agtccaagcg gcagcccatc ctggatgcca tagaggctaa gaagagacgg
     3661 caacaaaact tctgagcaat tccgcccctc tccctccccc ccccctaacg ttactggccg
     3721 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     3781 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     3841 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     3901 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     3961 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     4021 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     4081 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4141 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     4201 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     4261 tggccacaac catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4321 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4381 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     4441 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     4501 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4561 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4621 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4681 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4741 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     4801 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4861 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     4921 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     4981 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5041 atcgccttct tgacgagttc ttctgaacgc gttaagtcga caatcaacct ctggattaca
     5101 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5161 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5221 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5281 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5341 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5401 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5461 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5521 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5581 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5641 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     5701 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5761 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5821 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5881 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5941 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
     6001 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     6061 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6121 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6181 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6241 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6301 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     6361 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     6421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6541 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6601 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6661 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6721 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6781 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6841 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6901 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6961 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     7021 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7081 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7141 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7201 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7261 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7321 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7381 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7441 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7501 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7561 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7621 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7681 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7741 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7801 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7861 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7921 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7981 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8041 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8161 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8221 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8281 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8341 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8401 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8461 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8521 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8581 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8641 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8701 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8761 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8821 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8881 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8941 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9001 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9061 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9121 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     9181 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
