LOCUS       sequence-456619-        9474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-MST2
FEATURES             Location/Qualifiers
     source          1..9474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             2865..2873
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    join(3697..4271,4272..4274,4275..4283)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3864..3881)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4091..4110
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4284..5303
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    5331..5919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5384..5404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5802..5813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5991..6224
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6302..6423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6339..6358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6393..6412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6463..6598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6618..6637)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6619..6637)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6647..6664)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6647..6663)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6656..6678)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6805..7260
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6892..6911)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7102..7123
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7286..7390
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7391..7459,7460..8251)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7609..7628)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8422..9010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8911..8930
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9164..9181
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9298..9319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9334..9339,9340..9357,9358..9364)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9372..9388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9377..9399
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9396..9412
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9396..9412
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9431..9451
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9433..9451
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccctacgac
     2161 gtgcccgact acgccggcta tccgtatgat gtcccggact atgcaggaag cggagagcag
     2221 ccgccggcgc ctaagagtaa actaaaaaag ctgagtgaag acagtttgac taagcagcct
     2281 gaagaagttt ttgatgtatt agagaagctt ggagaagggt cttatggaag tgtatttaaa
     2341 gcaatacaca aggaatccgg tcaagttgtc gcaattaaac aagtacctgt tgaatcagat
     2401 cttcaggaaa taatcaaaga aatttccata atgcagcaat gtgacagccc atatgttgta
     2461 aagtactatg gcagttattt taagaataca gacctctgga ttgttatgga gtactgtggc
     2521 gctggctctg tctcagacat aattagatta cgaaacaaga cattaataga agatgaaatt
     2581 gcaaccattc ttaaatctac attgaaagga ctagaatatt tgcactttat gagaaaaata
     2641 cacagagata taaaagctgg aaatattctc ctcaatacag aaggacatgc aaaattggca
     2701 gattttggag tggctggtca gttaacagat acaatggcaa aacgcaatac tgtaatagga
     2761 actccatttt ggatggctcc tgaggtgatt caagaaatag gctataactg tgtggccgac
     2821 atctggtccc ttggcattac ttctatagaa atggctgaag gaaaacctcc ttatgctgat
     2881 atacatccaa tgagggctat ttttatgatt cccacaaatc caccaccaac attcagaaag
     2941 ccagaacttt ggtccgatga tttcaccgat tttgttaaaa agtgtttggt gaagaatcct
     3001 gagcagagag ctactgcaac acaactttta cagcatcctt ttatcaagaa tgccaaacct
     3061 gtatcaatat taagagacct gatcacagaa gctatggaga tcaaagctaa aagacatgag
     3121 gaacagcaac gagaattgga agaggaagaa gaaaattcgg atgaagatga gctggattcc
     3181 cacaccatgg tgaagactag tgtggagagt gtgggcacca tgcgggccac aagcacgatg
     3241 agtgaagggg cccagaccat gattgaacat aatagcacga tgttggaatc cgacttgggg
     3301 accatggtga taaacagtga ggatgaggaa gaagaagatg gaactatgaa aagaaatgca
     3361 acctcaccac aagtacaaag accatctttc atggactact ttgataagca agacttcaag
     3421 aataagagtc acgaaaactg taatcagaac atgcatgaac ccttccctat gtccaaaaac
     3481 gtttttcctg ataactggaa agttcctcaa gatggagact ttgacttttt gaaaaatcta
     3541 agtttagaag aactacagat gcggttaaaa gcactggacc ccatgatgga acgggagata
     3601 gaagaacttc gtcagagata cactgcgaaa agacagccca ttctggatgc gatggatgca
     3661 aagaaaagaa ggcagcaaaa cttttgagca attccgcccc tctccctccc ccccccctaa
     3721 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     3781 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     3841 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     3901 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     3961 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     4021 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     4081 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     4141 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     4201 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     4261 acgatgataa tatggccaca accatgaaaa agcctgaact caccgctacc tctgtcgaga
     4321 agtttctgat cgaaaagttc gacagtgtta gcgacctgat gcagctctcc gagggcgaag
     4381 aatctcgggc tttcagcttc gatgtgggag ggcgtggata tgtcctgcgg gtgaatagct
     4441 gcgccgatgg tttctacaaa gatcgctatg tttatcggca ctttgcatcc gccgctctcc
     4501 ctattcccga agtgcttgac attggggagt tcagcgagag cctgacctat tgcatctccc
     4561 gccgtgcaca gggtgtcacc ttgcaagacc tgcctgaaac cgaactgccc gctgttctcc
     4621 agcccgtcgc cgaggccatg gatgccatcg ctgccgccga tcttagccag accagcgggt
     4681 tcggcccatt cggacctcaa ggaatcggtc aatacactac atggcgcgat ttcatctgcg
     4741 ctattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcct
     4801 ccgtcgccca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     4861 acctcgtgca cgccgatttc ggctccaaca atgtcctgac cgacaatggc cgcataacag
     4921 ccgtcattga ctggagcgag gccatgttcg gggattccca atacgaggtc gccaacatct
     4981 tcttctggag gccctggttg gcttgtatgg agcagcagac ccgctacttc gagcggaggc
     5041 atcccgagct tgcaggatct cctcggctcc gggcttatat gctccgcatt ggtcttgacc
     5101 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggct cagggtcgct
     5161 gcgacgcaat cgtccggtcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     5221 gcgctgccgt ctggaccgat ggctgtgtgg aagtgctcgc cgatagtgga aacagacgcc
     5281 ccagcactcg tcctagggca aaggatctgc agtagacgcg ttaagtcgac aatcaacctc
     5341 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5401 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5461 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5521 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5581 ttgccaccac ctgtcaactc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5641 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5701 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     5761 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5821 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5881 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     5941 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6001 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6061 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6121 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6181 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     6241 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6301 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6361 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6421 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6481 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6541 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6601 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6661 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6721 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6781 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6841 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6901 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6961 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7021 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7081 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7141 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7201 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7261 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7321 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7381 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7441 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7501 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7561 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7621 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7681 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7741 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7801 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7861 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7921 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7981 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8041 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8101 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8161 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8221 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8281 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8341 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8401 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8461 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8521 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8581 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8641 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8701 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8761 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8821 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8881 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8941 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9001 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9061 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9121 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9181 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9241 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9301 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9361 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9421 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagc
//
