LOCUS       sequence-456620-       11808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Leng K, Rose IVL, Kim H, Xia W, Romero-Fernandez W, Rooney B, Koontz
            M, Li E, Ao Y, Wang S, Krawczyk M, Tcw J, Goate A, Zhang Y, Ullian 
            EM, Sofroniew MV, Fancy SPJ, Schrag MS, Lippmann ES, Kampmann M
  TITLE     CRISPRi screens in human iPSC-derived astrocytes elucidate 
            regulators of distinct inflammatory reactive states.
  JOURNAL   Nat Neurosci. 2022 Nov;25(11):1528-1542. doi: 
            10.1038/s41593-022-01180-9. Epub 2022 Oct 27.
  PUBMED    36303069
REFERENCE   2  (bases 1 to 11808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-022-01180-9"; journalName: "Nat Neurosci"; date: 
            "2022-11"; volume: "25"; issue: "11"; pages: "1528-1542"
COMMENT     Sequence Label: pKL123
FEATURES             Location/Qualifiers
     source          1..11808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(54..122,123..914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(272..291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1085..1673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1574..1593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1721..2557
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    2590..2608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2626..2644
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2662..2680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2698..2716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2734..2752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2770..2788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2806..2824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2869..2893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4512..4565
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    6112..6233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6149..6168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6203..6222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6282..6298)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6282..6298)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6295..6317)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6306..6322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6330..6336,6337..6354,6355..6360))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6375..6396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6513..6530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6822..6841
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    6840..6895
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     6941..6960
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(7051..7797)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7829..7848)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7856..8872
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7910..7932)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(8873..9150)
                     /label=chicken beta-actin promoter
     enhancer        9152..9531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    9537..9761
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9750..9767
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    9783..9816
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(9826..10851)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(10861..10914)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    10938..10963
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    10970..11003
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    11005..11808
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 aagggcgaat tcaaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
       61 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      121 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      181 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      241 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      301 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      361 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      421 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      481 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      541 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
      601 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      661 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      721 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      781 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      841 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      901 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      961 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1201 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1681 gcaacgcggc ctttttacgg ttcctggcga attcgccctt agagcagagc caggaacccc
     1741 tgtagggaag gggcaggaga gccaggggca tgagatggtg gacgaggaag ggggacaggg
     1801 aagcctgagc gcctctcctg ggcttgccaa ggactcaaac ccagaagccc agagcagggc
     1861 cttagggaag cgggaccctg ctctgggcgg aggaatatgt cccagatagc actggggact
     1921 ctttaaggaa agaaggatgg agaaagagaa agggagtaga ggcggccacg acctggtgaa
     1981 cacctaggac gcaccattct cacaaaggga gttttccaca cggacacccc cctcctcacc
     2041 acagccctgc caggacgggg ctggctactg gccttatctc acaggtaaaa ctgacgcacg
     2101 gaggaacaat ataaattggg gactagaaag gtgaagagcc aaagttagaa ctcaggacca
     2161 acttattctg attttgtttt tccaaactgc ttctcctctt gggaagtgta aggaagctgc
     2221 agcaccagga tcagtgaaac gcaccagaca gccgcgtcag agcagctcag gttctgggag
     2281 agggtagcgc agggtggcca ctgagaaccg ggcaggtcac gcatcccccc cttccctccc
     2341 accccctgcc aagctctccc tcccaggatc ctctctggct ccatcgtaag caaaccttag
     2401 aggttctggc aaggagagag atggctccag gaaatggggg tgtgtcacca gataaggaat
     2461 ctgcctaaca ggaggtgggg gttagaccca atatcaggag actaggaagg aggaggccta
     2521 aggatggggc ttttctgtca ccaatcctgt ccctagtaaa gctgatatct ccaccccaca
     2581 gtggtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2641 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2701 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     2761 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     2821 gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt
     2881 gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca actttccgta
     2941 ccacttccta ccctcgtaaa cttaaggccg ccaccatgta ttctccgctc tgtctcaccc
     3001 aggatgaatt tcatcctttc atcgaagcac ttctgcccca cgtccgagcc tttgcctaca
     3061 catggttcaa cctgcaggcc cgaaaacgaa aatacttcaa aaaacatgaa aagcgtatgt
     3121 caaaagaaga agagagagcc gtgaaggatg aattgctaag tgaaaaacca gaggtcaagc
     3181 agaagtgggc atctcgactt ctggcaaagt tgcggaaaga tatccgaccc gaatatcgag
     3241 aggattttgt tcttacagtt acagggaaaa aacctccatg ttgtgttctt tccaacccag
     3301 accagaaagg caagatgcga agaattgact gcctccgcca ggcagataaa gtctggaggt
     3361 tggaccttgt tatggtgatt ttgtttaaag gtattccgct ggaaagtact gatggcgagc
     3421 gccttgtaaa gtccccacaa tgctctaatc cagggctctg tgtccaaccc catcacatag
     3481 gggtttctgt taaggaactc gatttatatt tggcatactt tgtgcatgca gcagattcaa
     3541 gtcaatctga aagtcccagc cagccaagtg acgctgacat taaggaccag ccagaaaatg
     3601 gacatttggg cttccaggac agttttgtca catcaggtgt ttttagtgtc actgagctag
     3661 taagagtgtc acagacacca atagctgcag gaactggccc aaatttttct ctctcagatt
     3721 tggaaagttc ttcatactac agcatgagtc caggagcaat gaggaggtct ttacccagca
     3781 catcctctac gagctccaca aagcgcctca agtctgtgga ggatgaaatg gacagtcctg
     3841 gtgaggagcc attttataca ggccaagggc gctccccagg aagtggcagt cagtcaagtg
     3901 gatggcatga agtggagcca ggaatgccat ctccaaccac actgaagaag tcggagaagt
     3961 ctggtttcag cagcccctcc ccttcacaga cctcctccct gggaacggcg ttcacacagc
     4021 atcaccgacc tgtcattaca ggacccagag caagtccgca tgcaacacca tcgactcttc
     4081 atttcccgac atcacccatt atccagcagc ctgggcctta cttctcacac ccagccatcc
     4141 gctatcaccc tcaggagacg ctgaaagaat ttgtccaact tgtctgccct gatgctggtc
     4201 agcaggctgg acaggtgggg ttcctcaatc ccaatgggag cagccaaggc aaggtgcaca
     4261 acccattcct tcccacccca atgttgccac cgccaccgcc accaccgatg gccaggcctg
     4321 tgcctctgcc ggtgccagac acaaagcctc caaccacgtc aacagaagga ggtgcagcct
     4381 cccccacgtc accaacctac tcgacaccca gcacctcccc cgcaaaccga ttcgtcagtg
     4441 ttggaccacg ggatccaagc tttgtaaata tccctcaaca gacacagtcc tggtacctgg
     4501 gaggtagcgg agagggcaga ggcagtctgc tgacatgcgg tgacgtggaa gagaatcccg
     4561 gccctatgaa tctcctggac cccttcatga agatgaccga cgagcaggag aagggcctgt
     4621 ccggcgcccc cagccccacc atgtccgagg actccgcggg ctcgccctgc ccgtcgggct
     4681 ccggctcgga caccgagaac acgcggcccc aggagaacac gttccccaag ggcgagcccg
     4741 atctgaagaa ggagagcgag gaggacaagt tccccgtgtg catccgcgag gcggtcagcc
     4801 aggtgctcaa aggctacgac tggacgctgg tgcccatgcc ggtgcgcgtc aacggctcca
     4861 gcaagaacaa gccgcacgtc aagcggccca tgaacgcctt catggtgtgg gcgcaggcgg
     4921 cgcgcaggaa gctcgcggac cagtacccgc acttgcacaa cgccgagctc agcaagacgc
     4981 tgggcaagct ctggagactt ctgaacgaga gcgagaagcg gcccttcgtg gaggaggcgg
     5041 agcggctgcg cgtgcagcac aagaaggacc acccggatta caagtaccag ccgcggcgga
     5101 ggaagtcggt gaagaacggg caggcggagg cagaggaggc cacggagcag acgcacatct
     5161 cccccaacgc catcttcaag gcgctgcagg ccgactcgcc acactcctcc tccggcatga
     5221 gcgaggtgca ctcccccggc gagcactcgg ggcaatccca gggcccaccg accccaccca
     5281 ccacccccaa aaccgacgtg cagccgggca aggctgacct gaagcgagag gggcgcccct
     5341 tgccagaggg gggcagacag ccccctatcg acttccgcga cgtggacatc ggcgagctga
     5401 gcagcgacgt catctccaac atcgagacct tcgatgtcaa cgagtttgac cagtacctgc
     5461 cgcccaacgg ccacccgggg gtgccggcca cgcacggcca ggtcacctac acgggcagct
     5521 acggcatcag cagcaccgcg gccaccccgg cgagcgcggg ccacgtgtgg atgtccaagc
     5581 agcaggcgcc gccgccaccc ccgcagcagc ccccacaggc cccgccggcc ccgcaggcgc
     5641 ccccgcagcc gcaggcggcg cccccacagc agccggcggc acccccgcag cagccacagg
     5701 cgcacacgct gaccacgctg agcagcgagc cgggccagtc ccagcgaacg cacatcaaga
     5761 cggagcagct gagccccagc cactacagcg agcagcagca gcactcgccc caacagatcg
     5821 cctacagccc cttcaacctc ccacactaca gcccctccta cccgcccatc acccgctcac
     5881 agtacgacta caccgaccac cagaactcca gctcctacta cagccacgcg gcaggccagg
     5941 gcaccggcct ctactccacc ttcacctaca tgaaccccgc tcagcgcccc atgtacaccc
     6001 ccatcgccga cacctctggg gtcccttcca tcccgcagac ccacagcccc cagcactggg
     6061 aacaacccgt ctacacacag ctcactcgac cttgaatcga tcgcccaccc caacttgttt
     6121 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6181 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6241 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6301 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6361 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6421 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6481 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6541 gttcggctgc ggcgagcggt atcagctcac tcaaaggacg gccgcacatg taaggaaaat
     6601 tttagggatg ttaaagaaaa aaataacaca aaacaaaata taaaaaaaat ctaacctcaa
     6661 gtcaaggctt ttctatggaa taaggaatgg acagcagggg gctgtttcat atactgatga
     6721 cctctttata gccaaccttt gttcatggca gccagcatat gggcatatgt tgccaaactc
     6781 taaaccaaat actcattctg atgttttaaa tgatttgccc tcccatatgt ccttccgagt
     6841 gagagacaca aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca
     6901 gaagtcagat gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga
     6961 tctcagtggt atttgtgagc cagggcattg gccacaccag ccaccacctt ctgataggca
     7021 gcctgcacct gaggagtgaa ttcacgcgtt ttagcctggc agcatatcga gatcgaagtc
     7081 atcgagagcg tcagcaggga gcatatccag atcaaaatca tccagggcat cggcgggcag
     7141 catgtccagg tcgaagtcgt ccagggcgtc ggtgggtccg ccgctctcgc acttcagctg
     7201 cttctcgagg ccgcagatga tcagttccag gccgaacagg aaggcgggct cggcgccctg
     7261 tctgtcgaac agctcgatgg cctgcttcag cagggggggc atgctgtcgg tggtgggtgt
     7321 ctctctttcc tctttggcga cctggtgttc ctgttcttcc agcacgcagc ccagggtgaa
     7381 gtggcccacg gcgctcaggg cgtacagggc gttttccagg ctgaagccct gctggcacag
     7441 gaaggccagc tggttttcca gtgtctcgta ctgcttctcg gtgggtctgg tgcccaggtg
     7501 cactttggcg ccgtcccggt ggctcagcag ggcgcatctg tagctcttgg cgttgttccg
     7561 caggaagtcc tgccagctct cgccttccag agggcagctg tgggtgtggt gccggtccag
     7621 catctcgatg ggcagggcgt ccagcagggc ccgcttgttc ttcacgtgcc agtacagggt
     7681 gggctgttcc acgcccagtt tctgggccag cttccgggtg gtcaggccct cgatgcccac
     7741 gccgttcagc agttccaggg cgctgttgat cactttgctc ttgtccagcc ggctcatgtc
     7801 gacattcttt gccaaaatga tgagacagca caataaccag cacgttgccc aggagctgta
     7861 ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg gtcacacgcc
     7921 agaagccgaa ccccgccctg ccccgtcccc cccgaaggca gccgtccccc cgcggacagc
     7981 cccgaggctg gagagggaga aggggacggc ggcgcggcga cgcacgaagg ccctccccgc
     8041 ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg cccgctagag ggggtgcggc
     8101 ggcgcctccc agatttcggc tccgcacaga tttgggacaa aggaagtccc tgcgccctct
     8161 cgcacgatta ccataaaagg caatggctgc ggctcgccgc gcctcgacag ccgccggcgc
     8221 tccgggggcc gccgcgcccc tcccccgagc cctccccggc ccgaggcggc cccgccccgc
     8281 ccggcacccc cacctgccgc caccccccgc ccggcacggc gagccccgcg ccacgccccg
     8341 tacggagccc cgcacccgaa gccgggccgt gctcagcaac tcggggaggg gggtgcaggg
     8401 ggggttgcag cccgaccgac gcgcccacac cccctgctca cccccccacg cacacacccc
     8461 gcacgcagcc tttgttcccc tcgcagcccc ccccgcaccg cggggcaccg cccccggccg
     8521 cgctcccctc gcgcacactg cggagcgcac aaagccccgc gccgcgcccg cagcgctcac
     8581 agccgccggg cagcgcggag ccgcacgcgg cgctccccac gcacacacac acgcacgcac
     8641 cccccgagcc gctccccccg cacaaagggc cctcccggag cccctcaagg ctttcacgca
     8701 gccacagaaa agaaacaagc cgtcattaaa ccaagcgcta attacagccc ggaggagaag
     8761 ggccgtcccg cccgctcacc tgtgggagta acgcggtcag tcagagccgg ggcgggcggc
     8821 gcgaggcggc ggcggagcgg ggcacggggc gaaggcagcg cgcagcgact cccgcccgcc
     8881 gcgcgcttcg ctttttatag ggccgccgcc gccgccgcct cgccataaaa ggaaactttc
     8941 ggagcgcgcc gctctgattg gctgccgccg cacctctccg cctcgccccg ccccgcccct
     9001 cgccccgccc cgccccgcct ggcgcgcgcc cccccccccc ccccgccccc atcgctgcac
     9061 aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg gagatgggga
     9121 gagtgaagca gaacgtgggg ctcacctcga ccatggtaat agcgatgact aatacgtaga
     9181 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     9241 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     9301 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     9361 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     9421 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     9481 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt cgagacccat
     9541 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     9601 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     9661 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
     9721 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt
     9781 ttataacttc gtataatgta tgctatacga agttatggta ccttattatt ccttggctct
     9841 gggtcttgtg gagggtctcc tgtttccaga gtcagccaga acttcaacac atccatcagt
     9901 ccaaacagca gcagaccttc ttgcaatttg agttcttcca acagttccag caccagacct
     9961 cacaatggca tcacatcttc cttgtgccca agcagcatca tcaaagtttc catcaaccag
    10021 agattgatac agttggtcca ggccaattct gagcatgtag gctctcagtc tgggggaacc
    10081 agccagttct gggtgtcttc tttcaaaata tcttgtttgt tgttccatgc aagccagcca
    10141 aggtctccaa aaaaaaatgt tggcaacctc atattgagaa tctccaaaca tggcttcaga
    10201 ccagtcaatg actgcagtga ttctgccatt gtctgtcaga acattgttgc ttccaaaatc
    10261 agcatggacc aggtgtctga cttcaggaca atcttctgcc cacagcatga gttcatccag
    10321 tgcctgagca acagaagcag aaactgtgtc atccatcaca gtctgccagt gatagacatg
    10381 aggatcagca atggcacaaa tgaaatccct ccaagtggtg tactgaccga tgccttgggg
    10441 accaaaagga ccaaatccgc tggtttggct cagatcagct gctgcaatgg catccattgc
    10501 ttcagcaaca ggttgcagaa cagctggcag ctcagtttca gggagatcct ggagagtgac
    10561 tccttgtgct cttctgctga tgcagtaggt gagagattca gaaaattctc caatgtccag
    10621 aacttctgga attggcagag cagcagaggc aaagtgtctg taaacatatc tgtctttgta
    10681 aaaaccatca gcacaagaat tgaccctcag aacataacct cttcctccaa catcaaaaga
    10741 aaaggctctg ctttcttcac cttcagacag ctgcatgaga tcagaaacag aatcaaattt
    10801 ttcaatgaga aacttctcaa cagaagttgc tgtcagttca ggtttcttca tctcgagcct
    10861 agggccggga ttctcctcca cgtcaccgca tgttagaaga cttcctctgc cctctccgct
    10921 gccagatctc tcgaggccct gtgggaggaa gagaagaggt cagaagctta taacttcgta
    10981 taatgtatgc tatacgaagt tattgcccca ctgtggggtg gaggggacag ataaaagtac
    11041 ccagaaccag agccacatta accggccctg ggaatataag gtggtcccag ctcggggaca
    11101 caggatccct ggaggcagca aacatgctgt cctgaagtgg acataggggc ccgggttgga
    11161 ggaagaagac tagctgagct ctcggacccc tggaagatgc catgacaggg ggctggaaga
    11221 gctagcacag actagagagg taaggggggt aggggagctg cccaaatgaa aggagtgaga
    11281 ggtgacccga atccacagga gaacggggtg tccaggcaaa gaaagcaaga ggatggagag
    11341 gtggctaaag ccagggagac ggggtacttt ggggttgtcc agaaaaacgg tgatgatgca
    11401 ggcctacaag aaggggaggc gggacgcaag ggagacatcc gtcggagaag gccatcctaa
    11461 gaaacgagag atggcacagg ccccagaagg agaaggaaaa gggaacccag cgagtgaaga
    11521 cggcatgggg ttgggtgagg gaggagagat gcccggagag gacccagaca cggggaggat
    11581 ccgctcagag gacatcacgt ggtgcagcgc cgagaaggaa gtgctccgga aagagcatcc
    11641 ttgggcagca acacagcaga gagcaagggg aagagggagt ggaggaagac ggaacctgaa
    11701 ggaggcggca gggaaggatc tgggccagcc gtagaggtga cccaggccac aagctgcaga
    11761 cagaaagcgg cacaggccca ggggagagaa tgctggtcag agaaagca
//
