LOCUS       sequence-456649-        6310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter vector with wild type 5' Element (MMTV) insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Khader TA, Ahmad W, Akhlaq S, Panicker NG, Gull B, Baby J, Rizvi TA,
            Mustafa F
  TITLE     Transactivation of the novel 5' cis-acting element of mouse mammary 
            tumor virus (MMTV) by human retroviral transactivators Tat and Tax.
  JOURNAL   Commun Biol. 2024 Nov 16;7(1):1521. doi: 10.1038/s42003-024-07139-9.
  PUBMED    39550519
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-11-16"; volume: "7(1):1521. doi"; pages: " 
            10.1038/s42003-024-07139-9"
COMMENT     Sequence Label: TAK8
FEATURES             Location/Qualifiers
     source          1..6310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(642..1433,1434..1502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1265..1284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1503..1607)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1634..2089
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1721..1740)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1931..1952
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2220..2268
                     /note="synthetic polyadenylation signal"
     misc_feature    2282..2373
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2322..2341
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     primer_bind     complement(2399..2418)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2590..2969
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2970..3173
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3124..3144
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3954..3963
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3960..5612
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4026..4044)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5653..5774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5664..5683)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5718..5737
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6022..6039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6193..6310,1..471))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6273..6292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
       61 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      301 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
      721 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
      781 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
      841 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
      901 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
      961 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1021 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1081 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1141 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1201 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1261 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1321 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1381 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1441 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1501 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1561 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1621 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1681 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1741 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1801 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1861 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1921 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     1981 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     2041 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca
     2101 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2161 cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca
     2221 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac
     2281 taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc
     2341 ccagtgcaag tgcaggtgcc agaacatttc tctatcgata ggtaccgtcg actctcagta
     2401 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     2461 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     2521 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     2581 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     2641 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     2701 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2761 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2821 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2881 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2941 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     3001 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     3061 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     3121 tgacgcaaat gggcggtagg cgtgtacggt gggagttcta tataagcaga gctcgagtaa
     3181 acttgcaaca gtcctaatat tcacgtctcg tgtgtttgtg tctgttcgcc atcccgtctc
     3241 cgctcgtcac ttatccttca ctttccagag ggtccccccg cagaccccgg tgaccctcag
     3301 gtcggccgac tgcggcagct ggcgcccgaa cagggaccct cggataagtg acccttgtct
     3361 ctatttctac tatttggtgt ttgtcttgta ttgtctcttt cttgtctggc tatcatcaca
     3421 agagcggaac ggactcacca tagggagctg cagtcccgcc tacggagaag aggtaggtta
     3481 cggtgagcca ttggaaatgg gggtctcggg ctcaaaaggg cagaaactct ttgtttctgt
     3541 tttacaaagg ctcctctcag agaggggtct tcatgtgaaa gagagtagtg caatagaatt
     3601 ttatcagttt ctaataaagg tttctccttg gtttcccgaa gaaggaggat taaatttaca
     3661 agattggaaa agggtaggaa gagaaatgaa gaggtacgca gcggaacatg ggacagatag
     3721 cataccaaaa caggcttacc ccatttggct tcagttgaga gagatactga cagagcaatc
     3781 agacttggtt ttgctatccg cagaagccaa atctgttacc gaagaagaat tagaggaagg
     3841 tttaaccgga ctactatcga caagttcaca agaaaaaact tatgggacca ggggaacgat
     3901 gggctcgaga tctgcgatct aagtaagctt ggcattccgg tactgttggt aaagccacca
     3961 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
     4021 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
     4081 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
     4141 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
     4201 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
     4261 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt
     4321 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
     4381 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat
     4441 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
     4501 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat
     4561 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc
     4621 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
     4681 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
     4741 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca
     4801 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga
     4861 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta
     4921 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg
     4981 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
     5041 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
     5101 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt
     5161 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
     5221 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc
     5281 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac
     5341 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc
     5401 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
     5461 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
     5521 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
     5581 aggccaagaa gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc
     5641 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     5701 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5761 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     5821 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     5881 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     5941 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     6001 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6061 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6121 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6181 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6241 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6301 ctccctcgtg
//
