LOCUS       sequence-456651-        8002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter vector with MMTV 5' Element cloned downstream of
            luciferase gene in pHMR-Delta-eluc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Khader TA, Ahmad W, Akhlaq S, Panicker NG, Gull B, Baby J, Rizvi TA,
            Mustafa F
  TITLE     Transactivation of the novel 5' cis-acting element of mouse mammary 
            tumor virus (MMTV) by human retroviral transactivators Tat and Tax.
  JOURNAL   Commun Biol. 2024 Nov 16;7(1):1521. doi: 10.1038/s42003-024-07139-9.
  PUBMED    39550519
REFERENCE   2  (bases 1 to 8002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-11-16"; volume: "7(1):1521. doi"; pages: " 
            10.1038/s42003-024-07139-9"
COMMENT     Sequence Label: TAK4
FEATURES             Location/Qualifiers
     source          1..8002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    662..709
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     734..753
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(941..1735)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(941..1735)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1052..1071)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1662..1681
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1770..2127)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1784..1919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        1936..2127
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     2084..2104
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(2129..2233)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2260..2715
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2347..2366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2557..2578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2722..2843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(2966..3679,3680..3682,3683..3685))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2969..3679,3680..3682,3683..3685))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3011..3032)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3360..3379
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3619..3640
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3682..3691
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3761..3882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5681..6616)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     promoter        complement(7065..7268)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7094..7114)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7269..7572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(join(7747..8002,1..333))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7827..7846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
       61 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      481 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg
      541 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc
      601 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta
      661 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
      721 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag
      781 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
      841 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
      901 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
      961 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     1021 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     1081 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     1141 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     1201 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     1261 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     1321 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     1381 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     1441 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     1501 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
     1561 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     1621 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     1681 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     1741 acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct
     1801 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     1861 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     1921 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     1981 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     2041 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     2101 ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt
     2161 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2221 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     2281 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2341 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2401 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2461 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2521 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2581 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2641 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2701 taacgcttac aatttacgcc ttaagataca ttgatgagtt tggacaaacc acaactagaa
     2761 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     2821 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     2881 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     2941 attatgatct agagtcgcgg ccgctttact tgtacagctc gtccatgccg agagtgatcc
     3001 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     3061 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     3121 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     3181 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     3241 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     3301 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     3361 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     3421 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     3481 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     3541 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     3601 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     3661 acagctcctc gcccttgctc accatggtgg cgatggatcc cgggcccgcg gtaccgtcga
     3721 ctgcagaatt cgaagcttca aaaatatagt aggccactat taagatacat tgatgagttt
     3781 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3841 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3901 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     3961 tacaaatgtg gtatggctga ttatgatcta gagtcgcggc ctgactgaca gtcgcgcgag
     4021 tactgttccc ctggtcccat aagttttttc ttgtgaactt gtcgatagta gtccggttaa
     4081 accttcctct aattcttctt cggtaacaga tttggcttct gcggatagca aaaccaagtc
     4141 tgattgctct gtcagtatct ctctcaactg aagccaaatg gggtaagcct gttttggtat
     4201 gctatctgtc ccatgttccg ctgcgtacct cttcatttct cttcctaccc ttttccaatc
     4261 ttgtaaattt aatcctcctt cttcgggaaa ccaaggagaa acctttatta gaaactgata
     4321 aaattctatt gcactactct ctttcacatg aagacccctc tctgagagga gcctttgtaa
     4381 aacagaaaca aagagtttct gcccttttga gcccgagacc cccatttcca atggctcacc
     4441 gtaacctacc tcttctccgt aggcgggact gcagctccct atggtgagtc cgttccgctc
     4501 ttgtgatgat agccagacaa gaaagagaca atacaagaca aacaccaaat agtagaaata
     4561 gagacaaggg tcacttatcc gagggtccct gttcgggcgc cagctgccgc agtcggccga
     4621 cctgagggtc accggggtct gcggggggac cctctggaaa gtgaaggata agtgacgagc
     4681 ggagacggga tggcgaacag acacaaacac acgagacgtg aatattagga ctgttgcctc
     4741 gaggctcttc gcaaggcact ggaaaacaat ggggaaaatc ataagcacta tgactaaaag
     4801 cagggctcca acacctataa aaatgaaata ttgtgtccaa tctaatggat ttaaagcctt
     4861 tactccattg gcaaaggact gagccaagcc actgaggtcc actgtgtcaa tatgttgttt
     4921 gctcatatca ctaatcaggt tggttaattc ttgtatgtta tatgaaatct cattgtcatt
     4981 ccaaatgccc aataaatgag ctttggttct ttcccagctc tcagaagcat tatatggtaa
     5041 aggtgtaacg cagataaaat cataatttgc atgacacctg gtggacattc tggtctttaa
     5101 gtttgccaca tcttgtccca actctaaaac tacttcttct aaagcattaa gtctagcttc
     5161 taattttaaa tctattattc tttgttcaga taaagctaat gtaacattcc tatgaagatt
     5221 attaacaaac gtagcagttt gcatctcctt aactaaagca gtagtagcta cagcaaagga
     5281 agtgataata gcaattaaag cagatatgcc cagaataatg gcagcgacga atcgcttggc
     5341 tcgaattaaa tctgtggcat acctaaaggt ttgaatggca gaatcatcaa accatggttc
     5401 atcaccaata tctacaggta gcagcacgta tggcggcctc ttgactatga tcgctgcata
     5461 gtcgtaggca gaagaatcta aacaattagt caatctacaa gaagaacagg aaatatgaaa
     5521 agtagatcct cttttctcta tatctattag ctgaggtaat cctaataata tggcatgagg
     5581 gtaagtaaca caggcagatg taggaatcat gtcatgttct tgaaatcctg gccttttgag
     5641 aataagatat cttgaggctg caactaagcg aaacctagaa ttattgttca tttttgagaa
     5701 ctcgctcaac gaacgatttg atatattttc ccatttcatc aggtgcatct tcttgcgaaa
     5761 aatgaagacc ttttactttg acaaattcag tattaggaaa cttcttggca ccttcaacaa
     5821 tagcattgga aaagaatcct gggtccgatt caataaacat ttttggtaaa tcatcacttg
     5881 cacgtagata agcattataa ttcctaacaa tttgtacaac gtcaggttta ccacctttta
     5941 ctaacgggat ttcacgaggc catgataatg ttggacgacg aacttcacct ttctctttga
     6001 atggttcaag atatgctgca aattcttctg gttctaactt tctcatgatt tttgatggca
     6061 acatggtttc cacgaagaag ttattctcca aaaccatttt ttctccttct tcagatttga
     6121 tcaacgcaat atcttcttca atatcaggcc attcatccca tgattcaatc acatctacta
     6181 cactttcagc gtgaactatt gctttgatct tatcttgatg ctcatagcta taatgaaatg
     6241 ccaaacaagc accccaatca tggccgacaa aaatgatctt ctttggtaaa ttaagaagtt
     6301 caaaccatgc agtaagatat ttgtaatgat caagtaacct ataagaacca ttaccagatt
     6361 tgcctgattt gcccatacca ataaggtctg gtataataca ccgcgctact ggctcaatat
     6421 gtggcacaac atgtcgccat aaataagaag aggccgcgtt accatgtaaa aaaataacag
     6481 cattttctgc atgtttttct gaatcataat aattaataaa tgaatcaaga acattcattt
     6541 gtttacatct ggcccaccac tgcggaccag ttatcatccg tttcctttgt tctggatcat
     6601 aaactttcga agtcatggtg gctagaattc tgtatgggga actagagcag taaaatctct
     6661 atcattggga tccttaggag aattttccca gaaccatgta ggttctaccc atcctgcttc
     6721 ataccatcga tgaacctctt tattgacagg gggagtataa tttccaaata gatccttttt
     6781 gcttttaatc tgatctgact gatctacact aggcgaggga agggagaaat cccaaagtaa
     6841 cccaagggtc ccttttggag aaaaactcac cccctggtca gggaaggcgc aaggcaacca
     6901 ccgtggagga gcagactcgt ctccctccca gaaggcgtcc ttcttaaagg cgatctggca
     6961 gtgaggatat ttggggggca acttcttttt tatagggacc agttttgtat tggcccctga
     7021 gttccccaaa gtagtcgcta gcggatctga cggttcacta aaccagctct gcttatatag
     7081 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     7141 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     7201 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     7261 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     7321 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     7381 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     7441 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     7501 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     7561 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     7621 tattaataac taatgcatgg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7981 tcgctccaag ctgggctgtg tg
//
