LOCUS       sequence-456652-        6248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter vector with HIV-1 TAR region cloned in pGL3 
            Basic vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Khader TA, Ahmad W, Akhlaq S, Panicker NG, Gull B, Baby J, Rizvi TA,
            Mustafa F
  TITLE     Transactivation of the novel 5' cis-acting element of mouse mammary 
            tumor virus (MMTV) by human retroviral transactivators Tat and Tax.
  JOURNAL   Commun Biol. 2024 Nov 16;7(1):1521. doi: 10.1038/s42003-024-07139-9.
  PUBMED    39550519
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-11-16"; volume: "7(1):1521. doi"; pages: " 
            10.1038/s42003-024-07139-9"
COMMENT     Sequence Label: TAK11
FEATURES             Location/Qualifiers
     source          1..6248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     104..123
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    180..271
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(212..231)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    285..333
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(464..919)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(601..622)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     813..832
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        946..1050
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1051..1119,1120..1911)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1269..1288)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2082..2670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2571..2590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2824..2841
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3089..3210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3126..3145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3180..3199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3251..4903)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     4819..4837
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      4900..4909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             5401..5581
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5597..5800)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5626..5646)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5801..6180
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg
       61 acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt
      121 gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcggta cctatcgata
      181 gagaaatgtt ctggcacctg cacttgcact ggggacagcc tattttgcta gtttgttttg
      241 tttcgttttg ttttgatgga gagcgtatgt tagtactatc gattcacaca aaaaaccaac
      301 acacagatgt aatgaaaata aagatatttt attgcggccg ctccaagtac ctcccgtacc
      361 ttaatattac ttacttatca tggtagcttg ggctggcgta atagcgaaga ggcccgcacc
      421 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcaaattgt aagcgttaat
      481 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
      541 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
      601 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
      661 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
      721 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
      781 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
      841 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
      901 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      961 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1021 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1081 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1141 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1201 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1261 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1321 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1381 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1441 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1501 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1561 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1621 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1681 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1741 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1801 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1861 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1921 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1981 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2041 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2101 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2161 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2221 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2281 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2461 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2641 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2701 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2761 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2821 cgcagcgagt cagtgagcga ggaagcggaa gagcgctgcc ggcacctgtc ctacgagttg
     2881 catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa
     2941 ggagctgact gggttgaagg ctctcaaggg catcggtcga cggatcctta tcgattttac
     3001 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     3061 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     3121 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3181 tggtttgtcc aaactcatca atgtatctta tcatgtctgc tcgaagcggc cggccgcccc
     3241 gactctagaa ttacacggcg atctttccgc ccttcttggc ctttatgagg atctctctga
     3301 tttttcttgc gtcgagtttt ccggtaagac ctttcggtac ttcgtccaca aacacaactc
     3361 ctccgcgcaa ctttttcgcg gttgttactt gactggcgac gtaatccacg atctcttttt
     3421 ccgtcatcgt ctttccgtgc tccaaaacaa caacggcggc gggaagttca ccggcgtcat
     3481 cgtcgggaag acctgcgaca cctgcgtcga agatgttggg gtgttggagc aagatggatt
     3541 ccaattcagc gggagccacc tgatagcctt tgtacttaat cagagacttc aggcggtcaa
     3601 cgatgaagaa gtgttcgtct tcgtcccagt aagctatgtc tccagaatgt agccatccat
     3661 ccttgtcaat caaggcgttg gtcgcttccg gattgtttac ataaccggac ataatcatag
     3721 gacctctcac acacagttcg cctctttgat taacgcccag cgttttcccg gtatccagat
     3781 ccacaacctt cgcttcaaaa aatggaacaa ctttaccgac cgcgcccggt ttatcatccc
     3841 cctcgggtgt aatcagaata gctgatgtag tctcagtgag cccatatcct tgcctgatac
     3901 ctggcagatg gaacctcttg gcaaccgctt ccccgacttc cttagagagg ggagcgccac
     3961 cagaagcaat ttcgtgtaaa ttagataaat cgtatttgtc aatcagagtg cttttggcga
     4021 agaaggagaa tagggttggc accagcagcg cactttgaat cttgtaatcc tgaaggctcc
     4081 tcagaaacag ctcttcttca aatctataca ttaagacgac tcgaaatcca catatcaaat
     4141 atccgagtgt agtaaacatt ccaaaaccgt gatggaatgg aacaacactt aaaatcgcag
     4201 tatccggaat gatttgattg ccaaaaatag gatctctggc atgcgagaat ctcacgcagg
     4261 cagttctatg aggcagagcg acacctttag gcagaccagt agatccagag gagttcatga
     4321 tcagtgcaat tgtcttgtcc ctatcgaagg actctggcac aaaatcgtat tcattaaaac
     4381 cgggaggtag atgagatgtg acgaacgtgt acatcgactg aaatccctgg taatccgttt
     4441 tagaatccat gataataatt ttttggatga ttgggagctt tttttgcacg ttcaaaattt
     4501 tttgcaaccc ctttttggaa acgaacacca cggtaggctg cgaaatgccc atactgttga
     4561 gcaattcacg ttcattataa atgtcgttcg cgggcgcaac tgcaactccg ataaataacg
     4621 cgcccaacac cggcataaag aattgaagag agttttcact gcatacgacg attctgtgat
     4681 ttgtattcag cccatatcgt ttcatagctt ctgccaaccg aacggacatt tcgaagtact
     4741 cagcgtaagt gatgtccacc tcgatatgtg catctgtaaa agcaattgtt ccaggaacca
     4801 gggcgtatct cttcatagcc ttatgcagtt gctctccagc ggttccatct tccagcggat
     4861 agaatggcgc cgggcctttc tttatgtttt tggcgtcttc catggtggct ttaccaacag
     4921 taccggaatg ccaagcttac ttagatcgca gatctcgagc ccgggcatct atcctttgat
     4981 gcacacaata gaggactgct attgtattat ataatgatct aagttcttct gatcctgtct
     5041 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtc tctaaaaggc
     5101 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttagtttat
     5161 attgtttctt tccccctggc cttaaccgaa ttttttccca tttatctaat tctcccccgc
     5221 ttaataccga cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt
     5281 ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc
     5341 ctgcgtcgag agatctcctc tggctttact ttcgctttca agtccctgtt cgggcgccac
     5401 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     5461 acaacagacg ggcacacact actttgagca ctcaaggcaa gctttattga ggcttaagca
     5521 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     5581 cagtacaggc gctagcagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     5641 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     5701 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     5761 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     5821 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     5881 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     5941 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     6001 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     6061 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     6121 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
     6181 aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat
     6241 tcttcatg
//
