LOCUS       sequence-456738-       13116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MET CDS with 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13116)
  AUTHORS   Cerqua M, Foiani M, Boccaccio C, Comoglio PM, Altintas DM
  TITLE     The integrated stress response drives MET oncogene overexpression in
            cancers.
  JOURNAL   EMBO J. 2025 Jan 7. doi: 10.1038/s44318-024-00338-4.
  PUBMED    39774381
REFERENCE   2  (bases 1 to 13116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2025 Jan 7. doi: 10.1038/s44318-024-00338-4."
COMMENT     Sequence Label: pLenti-Exon1-MET
FEATURES             Location/Qualifiers
     source          1..13116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     101..118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    235..256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(271..276,277..294,295..301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     314..336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     333..349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     333..349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     368..388
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        370..388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        433..812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        869..1060
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(892..912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1079..1305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1306..1486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1533..1658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2151..2384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2569..2613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2826..2943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3001..3407
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    join(8062..8636,8637..8639,8640..8648)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8229..8246)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8456..8475
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8649..8651,8652..8654,8655..9368)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8649..8651,8652..8654,8655..9365)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8694..8715)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8955..8974)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9302..9323
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9384..9972
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9437..9457)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9855..9866)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10044..10277
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10355..10476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10392..10411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10446..10465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10516..10651
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10671..10690)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10672..10690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10700..10717)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10700..10716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10709..10731)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10858..11313
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10945..10964)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11155..11176
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11339..11443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11444..11512,11513..12304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11662..11681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12475..13063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12964..12983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc
     2821 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2881 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2941 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gctagctgca
     3001 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
     3061 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
     3121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
     3181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
     3241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
     3301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
     3361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cggccggtcg aatcaagctt
     3421 atcgataccg tcgacggatc caccggtact agtatactcc ggaagacacg tgctggggcg
     3481 ggcaggcgag cgcctcagtc tggtcgcctg gcggtgcctc cggccccaac gcgcccgggc
     3541 cgccgcgggc cgcgcgcgcc gatgcccggc tgagtcactg gcagggcagc gcgcgtgtgg
     3601 gaaggggcgg agggagtgcg gccggcgggc gggcggggcg ctgggctcag ccgggccgca
     3661 ggtgacccgg aggccctcgc cgcccgcggc gccccgagcg ctttgtgagc agatgcggag
     3721 ccgagtggag ggcgcgagcc agatgcgggg cgacagctga cttgctgaga ggaggcgggg
     3781 aggcgcggag cgcgcgtgtg gtccttgcgc cgctgacttc tccactggtt cctgggcacc
     3841 gaaagataaa cctctcataa tgaaggcccc cgctgtgctt gcacctggca tcctcgtgct
     3901 cctgtttacc ttggtgcaga ggagcaatgg ggagtgtaaa gaggcactag caaagtccga
     3961 gatgaatgtg aatatgaagt atcagcttcc caacttcacc gcggaaacac ccatccagaa
     4021 tgtcattcta catgagcatc acattttcct tggtgccact aactacattt atgttttaaa
     4081 tgaggaagac cttcagaagg ttgctgagta caagactggg cctgtgctgg aacacccaga
     4141 ttgtttccca tgtcaggact gcagcagcaa agccaattta tcaggaggtg tttggaaaga
     4201 taacatcaac atggctctag ttgtcgacac ctactatgat gatcaactca ttagctgtgg
     4261 cagcgtcaac agagggacct gccagcgaca tgtctttccc cacaatcata ctgctgacat
     4321 acagtcggag gttcactgca tattctcccc acagatagaa gagcccagcc agtgtcctga
     4381 ctgtgtggtg agcgccctgg gagccaaagt cctttcatct gtaaaggacc ggttcatcaa
     4441 cttctttgta ggcaatacca taaattcttc ttatttccca gatcatccat tgcattcgat
     4501 atcagtgaga aggctaaagg aaacgaaaga tggttttatg tttttgacgg accagtccta
     4561 cattgatgtt ttacctgagt tcagagattc ttaccccatt aagtatgtcc atgcctttga
     4621 aagcaacaat tttatttact tcttgacggt ccaaagggaa actctagatg ctcagacttt
     4681 tcacacaaga ataatcaggt tctgttccat aaactctgga ttgcattcct acatggaaat
     4741 gcctctggag tgtattctca cagaaaagag aaaaaagaga tccacaaaga aggaagtgtt
     4801 taatatactt caggctgcgt atgtcagcaa gcctggggcc cagcttgcta gacaaatagg
     4861 agccagcctg aatgatgaca ttcttttcgg ggtgttcgca caaagcaagc cagattctgc
     4921 cgaaccaatg gatcgatctg ccatgtgtgc attccctatc aaatatgtca acgacttctt
     4981 caacaagatc gtcaacaaaa acaatgtgag atgtctccag catttttacg gacccaatca
     5041 tgagcactgc tttaatagga cacttctgag aaattcatca ggctgtgaag cgcgccgtga
     5101 tgaatatcga acagagttta ccacagcttt gcagcgcgtt gacttattca tgggtcaatt
     5161 cagcgaagtc ctcttaacat ctatatccac cttcattaaa ggagacctca ccatagctaa
     5221 tcttgggaca tcagagggtc gcttcatgca ggttgtggtt tctcgatcag gaccatcaac
     5281 ccctcatgtg aattttctcc tggactccca tccagtgtct ccagaagtga ttgtggagca
     5341 tacattaaac caaaatggct acacactggt tatcactggg aagaagatca cgaagatccc
     5401 attgaatggc ttgggctgca gacatttcca gtcctgcagt caatgcctct ctgccccacc
     5461 ctttgttcag tgtggctggt gccacgacaa atgtgtgcga tcggaggaat gcctgagcgg
     5521 gacatggact caacagatct gtctgcctgc aatctacaag gttttcccaa atagtgcacc
     5581 ccttgaagga gggacaaggc tgaccatatg tggctgggac tttggatttc ggaggaataa
     5641 taaatttgat ttaaagaaaa ctagagttct ccttggaaat gagagctgca ccttgacttt
     5701 aagtgagagc acgatgaata cattgaaatg cacagttggt cctgccatga ataagcattt
     5761 caatatgtcc ataattattt caaatggcca cgggacaaca caatacagta cattctccta
     5821 tgtggatcct gtaataacaa gtatttcgcc gaaatacggt cctatggctg gtggcacttt
     5881 acttacttta actggaaatt acctaaacag tgggaattct agacacattt caattggtgg
     5941 aaaaacatgt actttaaaaa gtgtgtcaaa cagtattctt gaatgttata ccccagccca
     6001 aaccatttca actgagtttg ctgttaaatt gaaaattgac ttagccaacc gagagacaag
     6061 catcttcagt taccgtgaag atcccattgt ctatgaaatt catccaacca aatcttttat
     6121 tagtggtggg agcacaataa caggtgttgg gaaaaacctg aattcagtta gtgtcccgag
     6181 aatggtcata aatgtgcatg aagcaggaag gaactttaca gtggcatgtc aacatcgctc
     6241 taattcagag ataatctgtt gtaccactcc ttccctgcaa cagctgaatc tgcaactccc
     6301 cctgaaaacc aaagcctttt tcatgttaga tgggatcctt tccaaatact ttgatctcat
     6361 ttatgtacat aatcctgtgt ttaagccttt tgaaaagcca gtgatgatct caatgggcaa
     6421 tgaaaatgta ctggaaatta agggaaatga tattgaccct gaagcagtta aaggtgaagt
     6481 gttaaaagtt ggaaataaga gctgtgagaa tatacactta cattctgaag ccgttttatg
     6541 cacggtcccc aatgacctgc tgaaattgaa cagcgagcta aatatagagt ggaagcaagc
     6601 aatttcttca accgtccttg gaaaagtaat agttcaacca gatcagaatt tcacaggatt
     6661 gattgctggt gttgtctcaa tatcaacagc actgttatta ctacttgggt ttttcctgtg
     6721 gctgaaaaag agaaagcaaa ttaaagatct gggcagtgaa ttagttcgct acgatgcaag
     6781 agtacacact cctcatttgg ataggcttgt aagtgcccga agtgtaagcc caactacaga
     6841 aatggtttca aatgaatctg tagactaccg agctactttt ccagaagatc agtttcctaa
     6901 ttcatctcag aacggttcat gccgacaagt gcagtatcct ctgacagaca tgtcccccat
     6961 cctaactagt ggggactctg atatatccag tccattactg caaaatactg tccacattga
     7021 cctcagtgct ctaaatccag agctggtcca ggcagtgcag catgtagtga ttgggcccag
     7081 tagcctgatt gtgcatttca atgaagtcat aggaagaggg cattttggtt gtgtatatca
     7141 tgggactttg ttggacaatg atggcaagaa aattcactgt gctgtgaaat ccttgaacag
     7201 aatcactgac ataggagaag tttcccaatt tctgaccgag ggaatcatca tgaaagattt
     7261 tagtcatccc aatgtcctct cgctcctggg aatctgcctg cgaagtgaag ggtctccgct
     7321 ggtggtccta ccatacatga aacatggaga tcttcgaaat ttcattcgaa atgagactca
     7381 taatccaact gtaaaagatc ttattggctt tggtcttcaa gtagccaaag gcatgaaata
     7441 tcttgcaagc aaaaagtttg tccacagaga cttggctgca agaaactgta tgctggatga
     7501 aaaattcaca gtcaaggttg ctgattttgg tcttgccaga gacatgtatg ataaagaata
     7561 ctatagtgta cacaacaaaa caggtgcaaa gctgccagtg aagtggatgg ctttggaaag
     7621 tctgcaaact caaaagttta ccaccaagtc agatgtgtgg tcctttggcg tgctcctctg
     7681 ggagctgatg acaagaggag ccccacctta tcctgatgta aacacctttg atataactgt
     7741 ttacttgttg caagggagaa gactcctaca acccgaatac tgcccagacc ccttatatga
     7801 agtaatgcta aaatgctggc accctaaagc cgaaatgcgc ccatcctttt ctgaactggt
     7861 gtcccggata tcagcaatct tctctacttt cattggggag cactatgtcc atgtgaacgc
     7921 tacttatgtg aacgtaaaat gtgtcgctcc atatccttct ctgttgtcat cagaagataa
     7981 cgctgatgat gaggtggaca cacgaccagc ctccttctgg gagacatcat agacgcgtat
     8041 gcatgagtaa ctgaggatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     8101 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     8161 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     8221 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     8281 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     8341 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     8401 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     8461 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     8521 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     8581 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     8641 ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     8701 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     8761 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     8821 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     8881 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     8941 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     9001 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     9061 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     9121 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     9181 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     9241 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     9301 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     9361 acaagtgatc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     9421 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     9481 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     9541 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     9601 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     9661 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     9721 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     9781 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     9841 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     9901 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     9961 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
    10021 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
    10081 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    10141 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    10201 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    10261 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
    10321 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
    10381 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10441 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
    10501 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    10561 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    10621 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
    10681 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
    10741 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
    10801 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
    10861 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    10921 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    10981 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    11041 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
    11101 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    11161 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
    11221 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
    11281 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
    11341 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    11401 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    11461 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    11521 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    11581 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    11641 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
    11701 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    11761 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    11821 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    11881 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    11941 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    12001 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    12061 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    12121 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    12181 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    12241 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    12301 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    12361 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    12421 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    12481 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    12541 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    12601 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    12661 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    12721 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    12781 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    12841 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    12901 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    12961 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    13021 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    13081 ctttttacgg ttcctggcct tttgctggcc ttttgc
//
