LOCUS       sequence-456741-       11757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Covian-Nares JF, Koushik SV, Puhl HL, Vogel SS
  TITLE     Membrane wounding triggers ATP release and dysferlin-mediated 
            intercellular calcium signaling.
  JOURNAL   J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-93.
  PUBMED    20442251
REFERENCE   2  (bases 1 to 11757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2010 Jun 1. 123(Pt 11):1884-93."
COMMENT     Sequence Label: DYSF-3HA
FEATURES             Location/Qualifiers
     source          1..11757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      911..920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    927..951
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1665..1676
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7233..7313
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(7348..7365)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7354..7578
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7624..8052
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7711..7730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7921..7942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8061..8081)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8066..8395
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8246..8381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8308..8327
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8462..9256
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8516..8535)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9126..9145
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9430..9551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9467..9486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9521..9540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9600..9616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9600..9616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9613..9635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9624..9640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9648..9654,9655..9672,9673..9678))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9693..9714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9831..9848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10002..10590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10082..10101)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10761..11552,11553..11621))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11384..11403
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11622..11726)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gctaccggtc gccaccatgg aattccccaa ctttgtacaa aaaagcaggc tcactagtgg
      961 taccgtttaa acgatcggcc gccaccatgc tgagggtctt catcctctat gccgagaacg
     1021 tccacacacc cgacaccgac atcagcgatg cctactgctc cgcggtgttt gcaggggtga
     1081 agaagagaac caaagtcatc aagaacagcg tgaaccctgt atggaatgag ggatttgaat
     1141 gggacctcaa gggcatcccc ctggaccagg gctctgagct tcatgtggtg gtcaaagacc
     1201 atgagacgat ggggaggaac aggttcctgg gggaagccaa ggtcccactc cgagaggtcc
     1261 tcgccacccc tagtctgtcc gccagcttca atgcccccct gctggacacc aagaagcagc
     1321 ccacaggggc ctcgctggtc ctgcaggtgt cctacacacc gctgcctgga gctgtgcccc
     1381 tgttcccgcc ccctactcct ctggagccct ccccgactct gcctgacctg gatgtagtgg
     1441 cagacacagg aggagaggaa gacacagagg accagggact cactggagat gaggcggagc
     1501 cattcctgga tcaaagcgga ggcccggggg ctcccaccac cccaaggaaa ctaccttcac
     1561 gtcctccgcc ccactacccc gggatcaaaa gaaagcgaag tgcgcctaca tctagaaagc
     1621 tgctgtcaga caaaccgcag gatttccaga tcagggtcca ggtgatcgag gggcgccagc
     1681 tgccgggggt gaacatcaag cctgtggtca aggttaccgc tgcagggcag accaagcgga
     1741 cgcggatcca caagggaaac agcccactct tcaatgagac tcttttcttc aacttgtttg
     1801 actctcctgg ggagctgttt gatgagccca tctttatcac ggtggtagac tctcgttctc
     1861 tcaggacaga tgctctcctc ggggagttcc ggatggacgt gggcaccatt tacagagagc
     1921 cccggcacgc ctatctcagg aagtggctgc tgctctcaga ccctgatgac ttctctgctg
     1981 gggccagagg ctacctgaaa acaagccttt gtgtgctggg gcctggggac gaagcgcctc
     2041 tggagagaaa agacccctct gaagacaagg aggacattga aagcaacctg ctccggccca
     2101 caggcgtagc cctgcgagga gcccacttct gcctgaaggt cttccgggcc gaggacttgc
     2161 cgcagatgga cgatgccgtg atggacaacg tgaaacagat ctttggcttc gagagtaaca
     2221 agaagaactt ggtggacccc tttgtggagg tcagctttgc ggggaaaatg ctgtgcagca
     2281 agatcttgga gaagacggcc aaccctcagt ggaaccagaa catcacactg cctgccatgt
     2341 ttccctccat gtgcgaaaaa atgaggattc gtatcataga ctgggaccgc ctgactcaca
     2401 atgacatcgt ggctaccacc tacctgagta tgtcgaaaat ctctgcccct ggaggagaaa
     2461 tagaagagga gcctgcaggt gctgtcaagc cttcgaaagc ctcagacttg gatgactacc
     2521 tgggcttcct ccccactttt gggccctgct acatcaacct ctatggcagt cccagagagt
     2581 tcacaggctt cccagacccc tacacagagc tcaacacagg caagggggaa ggtgtggctt
     2641 atcgtggccg gcttctgctc tccctggaga ccaagctggt ggagcacagt gaacagaagg
     2701 tggaggacct tcctgcggat gacatcctcc gggtggagaa gtaccttagg aggcgcaagt
     2761 actccctgtt tgcggccttc tactcagcca ccatgctgca ggatgtggat gatgccatcc
     2821 agtttgaggt cagcatcggg aactacggga acaagttcga catgacctgc ctgccgctgg
     2881 cctccaccac tcagtacagc cgtgcagtct ttgacgggtg ccactactac tacctaccct
     2941 ggggtaacgt gaaacctgtg gtggtgctgt catcctactg ggaggacatc agccatagaa
     3001 tcgagactca gaaccagctg cttgggattg ctgaccggct ggaagctggc ctggagcagg
     3061 tccacctggc cctgaaggcg cagtgctcca cggaggacgt ggactcgctg gtggctcagc
     3121 tgacggatga gctcatcgca ggctgcagcc agcctctggg tgacatccat gagacaccct
     3181 ctgccaccca cctggaccag tacctgtacc agctgcgcac ccatcacctg agccaaatca
     3241 ctgaggctgc cctggccctg aagctcggcc acagtgagct ccctgcagct ctggagcagg
     3301 cggaggactg gctcctgcgt ctgcgtgccc tggcagagga gccccagaac agcctgccgg
     3361 acatcgtcat ctggatgctg cagggagaca agcgtgtggc ataccagcgg gtgcccgccc
     3421 accaagtcct cttctcccgg cggggtgcca actactgtgg caagaattgt gggaagctac
     3481 agacaatctt tctgaaatat ccgatggaga aggtgcctgg cgcccggatg ccagtgcaga
     3541 tacgggtcaa gctgtggttt gggctctctg tggatgagaa ggagttcaac cagtttgctg
     3601 aggggaagct gtctgtcttt gctgaaacct atgagaacga gactaagttg gcccttgttg
     3661 ggaactgggg cacaacgggc ctcacctacc ccaagttttc tgacgtcacg ggcaagatca
     3721 agctacccaa ggacagcttc cgcccctcgg ccggctggac ctgggctgga gattggttcg
     3781 tgtgtccgga gaagactctg ctccatgaca tggacgccgg tcacctgagc ttcgtggaag
     3841 aggtgtttga gaaccagacc cggcttcccg gaggccagtg gatctacatg agtgacaact
     3901 acaccgatgt gaacggggag aaggtgcttc ccaaggatga cattgagtgc ccactgggct
     3961 ggaagtggga agatgaggaa tggtccacag acctcaaccg ggctgtcgat gagcaaggct
     4021 gggagtatag catcaccatc cccccggagc ggaagccgaa gcactgggtc cctgctgaga
     4081 agatgtacta cacacaccga cggcggcgct gggtgcgcct gcgcaggagg gatctcagcc
     4141 aaatggaagc actgaaaagg cacaggcagg cggaggcgga gggcgagggc tgggagtacg
     4201 cctctctttt tggctggaag ttccacctcg agtaccgcaa gacagatgcc ttccgccgcc
     4261 gccgctggcg ccgtcgcatg gagccactgg agaagacggg gcctgcagct gtgtttgccc
     4321 ttgagggggc cctgggcggc gtgatggatg acaagagtga agattccatg tccgtctcca
     4381 ccttgagctt cggtgtgaac agacccacga tttcctgcat attcgactat gggaaccgct
     4441 accatctacg ctgctacatg taccaggccc gggacctggc tgcgatggac aaggactctt
     4501 tttctgatcc ctatgccatc gtctccttcc tgcaccagag ccagaagacg gtggtggtga
     4561 agaacaccct taaccccacc tgggaccaga cgctcatctt ctacgagatc gagatctttg
     4621 gcgagccggc cacagttgct gagcaaccgc ccagcattgt ggtggagctg tacgaccatg
     4681 acacttatgg tgcagacgag tttatgggtc gctgcatctg tcaaccgagt ctggaacgga
     4741 tgccacggct ggcctggttc ccactgacga ggggcagcca gccgtcgggg gagctgctgg
     4801 cctcttttga gctcatccag agagagaagc cggccatcca ccatattcct ggttttgagg
     4861 tgcaggagac atcaaggatc ctggatgagt ctgaggacac agacctgccc tacccaccac
     4921 cccagaggga ggccaacatc tacatggttc ctcagaacat caagccagcg ctccagcgta
     4981 ccgccatcga gatcctggca tggggcctgc ggaacatgaa gagttaccag ctggccaaca
     5041 tctcctcccc cagcctcgtg gtagagtgtg ggggccagac ggtgcagtcc tgtgtcatca
     5101 ggaacctccg gaagaacccc aactttgaca tctgcaccct cttcatggaa gtgatgctgc
     5161 ccagggagga gctctactgc ccccccatca ccgtcaaggt catcgataac cgccagtttg
     5221 gccgccggcc tgtggtgggc cagtgtacca tccgctccct ggagagcttc ctgtgtgacc
     5281 cctactcggc ggagagtcca tccccacagg gtggcccaga cgatgtgagc ctactcagtc
     5341 ctggggaaga cgtgctcatc gacattgatg acaaggagcc cctcatcccc atccaggagg
     5401 aagagttcat cgattggtgg agcaaattct ttgcctccat aggggagagg gaaaagtgcg
     5461 gctcctacct ggagaaggat tttgacaccc tgaaggtcta tgacacacag ctggagaatg
     5521 tggaggcctt tgagggcctg tctgactttt gtaacacctt caagctgtac cggggcaaga
     5581 cgcaggagga gacagaagat ccatctgtga ttggtgaatt taagggcctc ttcaaaattt
     5641 atcccctccc agaagaccca gccatcccca tgcccccaag acagttccac cagctggccg
     5701 cccagggacc ccaggagtgc ttggtccgta tctacattgt ccgagcattt ggcctgcagc
     5761 ccaaggaccc caatggaaag tgtgatcctt acatcaagat ctccataggg aagaaatcag
     5821 tgagtgacca ggataactac atcccctgca cgctggagcc cgtatttgga aagatgttcg
     5881 agctgacctg cactctgcct ctggagaagg acctaaagat cactctctat gactatgacc
     5941 tcctctccaa ggacgaaaag atcggtgaga cggtcgtcga cctggagaac aggctgctgt
     6001 ccaagtttgg ggctcgctgt ggactcccac agacctactg tgtctctgga ccgaaccagt
     6061 ggcgggacca gctccgcccc tcccagctcc tccacctctt ctgccagcag catagagtca
     6121 aggcacctgt gtaccggaca gaccgtgtaa tgtttcagga taaagaatat tccattgaag
     6181 agatagaggc tggcaggatc ccaaacccac acctgggccc agtggaggag cgtctggctc
     6241 tgcatgtgct tcagcagcag ggcctggtcc cggagcacgt ggagtcacgg cccctctaca
     6301 gccccctgca gccagacatc gagcagggga agctgcagat gtgggtcgac ctatttccga
     6361 aggccctggg gcggcctgga cctcccttca acatcacccc acggagagcc agaaggtttt
     6421 tcctgcgttg tattatctgg aataccagag atgtgatcct ggatgacctg agcctcacgg
     6481 gggagaagat gagcgacatt tatgtgaaag gttggatgat tggctttgaa gaacacaagc
     6541 aaaagacaga cgtgcattat cgttccctgg gaggtgaagg caacttcaac tggaggttca
     6601 ttttcccctt cgactacctg ccagctgagc aagtctgtac cattgccaag aaggatgcct
     6661 tctggaggct ggacaagact gagagcaaaa tcccagcacg agtggtgttc cagatctggg
     6721 acaatgacaa gttctccttt gatgattttc tgggctccct gcagctcgat ctcaaccgca
     6781 tgcccaagcc agccaagaca gccaagaagt gctccttgga ccagctggat gatgctttcc
     6841 acccagaatg gtttgtgtcc ctttttgagc agaaaacagt gaagggctgg tggccctgtg
     6901 tagcagaaga gggtgagaag aaaatactgg cgggcaagct ggaaatgacc ttggagattg
     6961 tagcagagag tgagcatgag gagcggcctg ctggccaggg ccgggatgag cccaacatga
     7021 accctaagct tgaggaccca aggcgccccg acacctcctt cctgtggttt acctccccat
     7081 acaagaccat gaagttcatc ctgtggcggc gtttccggtg ggccatcatc ctcttcatca
     7141 tcctcttcat cctgctgctg ttcctggcca tcttcatcta cgccttcccg aactatgctg
     7201 ccatgaagct ggtgaagccc ttcagcgata tctacccata cgatgttcca gattacgctt
     7261 acccatacga tgttccagat tacgcttacc catacgatgt tccagattac gcttaatcta
     7321 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7381 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7441 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7501 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7561 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7621 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7801 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7921 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8041 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8101 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8161 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8221 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8281 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     8341 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8401 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     8461 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     8521 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     8581 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     8641 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     8701 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     8761 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     8821 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     8881 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     8941 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     9001 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     9061 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     9121 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     9181 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     9241 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     9301 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9361 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9421 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9481 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9541 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10441 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10561 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10621 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10681 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10741 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10801 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10861 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10921 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10981 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11041 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11101 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11161 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11221 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11281 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11341 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11401 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11461 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11521 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11581 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11641 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11701 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
