LOCUS       sequence-456756-        9732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Anthoceros agrestis Chaperonin 60-alpha, Chaperonin 
            60-beta, Chaperonin 20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Oh ZG, Robison TA, Loh DH, Ang WSL, Ng JZY, Li FW, Gunn LH
  TITLE     Unique biogenesis and kinetics of hornwort Rubiscos revealed by 
            synthetic biology systems.
  JOURNAL   Mol Plant. 2024 Dec 2;17(12):1833-1849. doi: 
            10.1016/j.molp.2024.10.013. Epub 2024 Nov 2.
  PUBMED    39491367
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molp.2024.10.013"; journalName: "Mol Plant"; date: 
            "2024-12-2- 2"; volume: "17"; issue: "12"; pages: "1833-1849"
COMMENT     Sequence Label: p11_AaC60-alpha-beta/C20
FEATURES             Location/Qualifiers
     source          1..9732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..36)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        104..208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(209..277,278..1069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(427..446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1729..1748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1982..1999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2014..2153
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2079..2098)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2239..2261
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2255..2446)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3758..4840)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4802..4821
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4841..4918)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5124..5143
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5227..5246
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5227..5245
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5246..5270
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5293..5315
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             6961..6983
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             8685..8707
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(9459..9477)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9473..9520
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(9695..9700,9701..9717,9718..9723))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca
     2101 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     2161 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2221 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2281 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
     2341 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     2401 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
     2461 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga
     2521 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
     2581 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
     2641 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca
     2701 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
     2761 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
     2821 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
     2881 tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc
     2941 gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     3001 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     3061 tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg
     3121 caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca
     3181 tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga tcgaagttag
     3241 gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct
     3301 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     3361 ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
     3421 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
     3481 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
     3541 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
     3601 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
     3661 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     3721 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3781 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3841 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     3901 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     3961 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     4021 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     4081 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     4141 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     4201 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     4261 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
     4321 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
     4381 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
     4441 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     4501 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     4561 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     4621 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     4681 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     4741 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     4801 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
     4861 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
     4921 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     4981 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     5041 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     5101 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     5161 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
     5221 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc ggaacattcc
     5281 ctctagaata attttgttta actttaagaa ggagatatac atatgcaggc caaggacatc
     5341 agtttcgatg gcaagtcgcg ggctgcgctg caggcaggga ttgacaagct cgctgacgcc
     5401 gtcgcggtga ccttaggtcc acggggcagg aacgtggtgc tggatgagtt cggcaccccc
     5461 aaggtcatca acgatggcgt caccattgcc cgggccatcg agcttcccga cgccatggag
     5521 aacgcaggag ctgcccttat ccgcgaggtg gctagtaaga caaatgattc tgctggggac
     5581 ggtaccacaa cagcatgcgt gttggcccgc gagctcatca ggttcggttt gttgaatgtt
     5641 acctctggtg ccagccctgt tgctatcaag aaggggatag acaagactgt gaccgcgctg
     5701 gtgaagaagc ttgcagagaa ggcgcggcct gtgcagggtc gggaggatat caaagccatt
     5761 gctgccattt cggccggaaa tgatgacttc gttgggacca tgattgctga tgctattgac
     5821 aaggtgggct ctgatggggt gctttctatc gagtcatcct cctcttttga gacgacggtg
     5881 gaggtggagg agggtatgga gattgacagg ggttacatct cccctcagtt tgtcaccaac
     5941 ccagaaaagt tgctggttga gttcgagaat gcgagagtgc ttgtcactga ccagaagatc
     6001 accaccatca aggacatcat tccagttttg gagaagacca cgcagctcag tgtgcctctc
     6061 ctcatcatcg cggaggatgt ttccggagaa gcgctagcaa ccttggtggt caataagctg
     6121 cgaggggttc tgcaagttgc tgctatcaag gctccagggt tcggagagcg ccgcaaggct
     6181 ttgcttcaag atattgccat cgtaacaggt gcagagtttc tggccagtga ccttggcatg
     6241 aaggtggaga atactagcag tgaccagctt ggtattgcta ggaagatcgt catcagcaac
     6301 ggcagtacaa ctatcattgc agatgctgcc accaaggacg agattcaggc tcgtgttgcc
     6361 cagatcaaga aggagctggc agagactgac tccgtgtacg attcagagaa gttgtcggag
     6421 cgaattgcaa agctgtcagg tggtgttgct gtcattaagg ttggagctgc cactgagaca
     6481 gagcttgagg acaggaagtt gagaattgag gacgcgaaga atgccacttt tgcagcgatt
     6541 gaggaaggca ttgtgcccgg aggaggtgct gccatggtgc atctgtctgc ctttgttccg
     6601 gagatcaagg cagggattga agatcctgaa gagaagcttg ggtctgacat tgtccagaag
     6661 gctctcctct ccccagcgtc tattattgcc agcaattcgg gtgtggaagg tgcagtggta
     6721 attgagaaaa ttttgagcag tgactgggaa tttgggtaca atgctatgac tgataagtat
     6781 gagaaccttc tggaaactgg cgtgattgac ccagctaagg ttacaagatg tgccctgcaa
     6841 aatgctgcct ctgttgctgg tatggtgctg acaacacagg ccatagtggt tgaaaagatc
     6901 aagaagaagc agcccgcagt tccacaagtc ccggggatga gcatgtaggc tagaaataat
     6961 tttgtttaac tttaagaagg agatatacat atgactcggg ctgccaagga gttgtacttc
     7021 aacaaggatg ggtccgcgac gaagaagttg caggctggtg tggacaagtt ggccaacttg
     7081 gttggagtca cgttgggacc taaggggcgg aatgtggtgc tggagagcaa gtatggctct
     7141 cccaagattg tcaacgatgg tgtgaccgtg gccaaggagg ttgagctgga ggacccagtt
     7201 gagaacattg gagctaagct tgtgagacag gctgctgcta agactaatga cttggctgga
     7261 gacggaacta ccacttccgt ggtgcttgcg caagggctga ttgcggaggg tgttaaggtt
     7321 gttgcagctg gtgccaatcc tatccagatc acccgtggta ttgaaaagac ggtagcggct
     7381 cttgttcagg agctaaagaa cattgccaag gatgtcgagg atagcgagct ggcagatgtt
     7441 gccgcagtga gtgcagggaa caatgaggaa gtcggtctta tgattgccga ggccatgagc
     7501 aaagttggaa ggaagggtgt tgttaccctc gaggaaggga agagtgccga gaacaatctg
     7561 tatgtggttg agggtatgca gtttgacaga ggttacattt ctccgtattt tgtaacggac
     7621 tcagagaaaa tgtctgtcga gtatgacaac tgcaagctcc tcattgtgga caagaaaatt
     7681 tcaacagcca gggacatgat cggaattcta gaagatgcca ttcggggtgg gtacccactt
     7741 ctcatcattg ctgaagacat tgagcaagaa gccctggcca ctttggtggt taacaagcta
     7801 cgaggcgcac tgaaggtggc tgctctcaaa gcccctggtt tcggggagcg gaagtcgcag
     7861 tacttggacg acattgcgtg cttgactgga ggaactgtgg ttagagatga agtgggtttg
     7921 tctttggaca aggtaggcag ggaggttctt gggaccgctg ccaaggtgga aatcacgaag
     7981 gatgctacca caatagtcgg tgatggaagc acccaagatg tggttgagaa gcgtgtggca
     8041 caaattcgga atctgataga gaatgccgag caagaatatg agaaggagaa attgaatgag
     8101 aggattgcca agctttcagg cggagtggca atcattcagg ttggagccca aactgagaca
     8161 gagctgaagg agaagaagct gcgagtggaa gatgccctca atgccacaaa ggctgctgta
     8221 gaggaaggaa ttgttgttgg tggtggatgc actcttttga gactggctgc caaagtggaa
     8281 gggatcaagt catccctgga caacgacgag caaagggttg gtgcggacat cgtgaagaga
     8341 gctcttagct atccactcaa gctgatagcc aagaatgcag gagtgaacgg aagtgttgtg
     8401 gtggagaagg ttttgtcaaa tgacaatcct aactttggct acaatgccgc tactgacaca
     8461 tacgaagacc tgatggctaa cggcattatc gacccggcca aggtcgtgag atgctgtctg
     8521 gaacatgctg cctccgtggc gaagacattc ctgacatcag actgtgttgt ggtggagatc
     8581 aaagaacctg aatcagctgt acctgccgga aaccctatgg ataactcagg atacggatac
     8641 tgaggtcgac gtcgaccgga taacaattgc cctctagaaa taattttgtt taactttaag
     8701 aaggagatat acatatggcc acagctgttg ctcctaagtt caagaccttg aagcctttgg
     8761 gcgaccggct ccttgtgaag attcaaactg tagaagagaa gacatctgga ggcattctgc
     8821 tcccctcgac tgcccagtcg aaacctcaag gcggtgaagt tgttgctgta ggagagggca
     8881 agacggttgg agacaagaag attgagcttg gtgtgaagac tggagattcc attgtgtact
     8941 ccaagtacgc tggcaccgag gttgagttca acggtgttga tcatcttctt ctaaaagaag
     9001 atgacatcgt tggccttctc agctccgagg acatcaagga tttgcagccc ttgaatgacc
     9061 gagtgttgat caagatcgag gagcctgagg agaagtcagc aggaggagtc ttgctcacag
     9121 acagtgccaa ggagaagcct gttattggaa cggttgtggc tgttggccct ggggttgtcg
     9181 gcgaagatgg cacgaggaag gctctggagc tctctgctgg aaacaccgtc ttgtattcca
     9241 agtacgctgg gaacgacttc aagagcaagg atggcacgca gtatgtggtc atgagggcta
     9301 cagatgtgct tgccgtcttg agctaattat gttatatctt tgtaatctgc aacaagctgg
     9361 gtaccggtac cgaaagaagg catgactggt ggacagcaaa tgggtcgcgg atccggctgc
     9421 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     9481 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     9541 cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
     9601 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
     9661 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt
     9721 caaacatgag aa
//
