LOCUS       sequence-456796-       19765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NST3 in different cell types in Arabidopsis thaliana upon 
            induction with dexamethasone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19765)
  AUTHORS   Tamadaddi C, Choi J, Ghasemi M, Kim SH, Gomez ED, Gomez EW, Anderson
            CT
  TITLE     NST3 induces ectopic transdifferentiation, forming secondary walls 
            with diverse patterns and composition in Arabidopsis thaliana.
  JOURNAL   Ann Bot. 2024 Aug 30:mcae153. doi: 10.1093/aob/mcae153.
  PUBMED    39212164
REFERENCE   2  (bases 1 to 19765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Ann Bot. 
            2024 Aug 30:mcae153. doi: 10.1093/aob/mcae153."
COMMENT     Sequence Label: NST3_pOpOn2.1
FEATURES             Location/Qualifiers
     source          1..19765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1949..3097)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  3722..4489
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     oriT            4549..4658
                     /note="incP origin of transfer"
     misc_feature    5213..5237
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(5300..5552)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(6177..6947,6948..6956,6957..6998))
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6288..6307)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6898..6917
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6999..7182)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     7340..7356
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7363..7381
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        7478..7822
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             9714..10058
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     regulatory      10556..10565
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     11223..11239
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11249..11267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(11556..13367)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFRD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     regulatory      13364..13373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(13479..13525)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    13595..13614
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    13647..13666
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    13699..13718
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    13751..13770
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    13803..13822
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    13855..13874
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        13898..13944
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    14060..14084
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(15198..15222)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      15263..15970
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(16003..16021)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(16004..16021)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    16059..16083
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      16571..17206
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(17881..18669)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgatatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatgaa gatcggcggc
      841 aatagcttct tagcgccatc ccggctgaga aagcccagta aggaaacaac tgtaggttcg
      901 agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat caggccgagc
      961 cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc aaacactaaa
     1021 gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga
     1081 ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga aaccgccccc
     1141 gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa caccaacagc
     1201 gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat cgggctacct
     1261 agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac cgtcgccgcg
     1321 accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag cgaaatatta
     1381 agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg tcggacgatc
     1441 atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac ccctcggcct
     1501 cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc cttggggccg
     1561 tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc
     1621 acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa
     1681 acggacccga acatctctgg agctttcttc agggccgaca atcggatctc gcggaaatcc
     1741 tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt caattttaat
     1801 cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc
     1861 aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg gctgctgaac
     1921 ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga
     1981 cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct
     2041 cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct
     2101 tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg
     2161 acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga
     2221 cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc
     2281 ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca
     2341 tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga
     2401 tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag
     2461 gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca
     2521 gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt
     2581 gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt
     2641 cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca
     2701 tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct
     2761 gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt
     2821 cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc gaacgctcca
     2881 cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct
     2941 tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca
     3001 tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt
     3061 cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc
     3121 cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt
     3181 ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct
     3241 cgtacttggt attccgaatc ttgccctgca cgaataccag ctccgcgaag tcgctcttct
     3301 tgatggagcg catggggacg tgcttggcaa tcacgcgcac cccccggccg ttttagcggc
     3361 taaaaaagtc atggctctgc cctcgggcgg accacgccca tcatgacctt gccaagctcg
     3421 tcctgcttct cttcgatctt cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc
     3481 gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc caggtcgcca
     3541 ttgatgcggg ccagctcgcg gacgtgctca tagtccacga cgcccgtgat tttgtagccc
     3601 tggccgacgg ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc cttttcctca
     3661 atcgctcttc gttcgtctgg aaggcagtac accttgatag gtgggctgcc cttcctggtt
     3721 gggtaatgac tccaacttat tgatagtgtt ttatgttcag ataatgcccg atgactttgt
     3781 catgcagctc caccgatttt gagaacgaca gcgacttccg tcccagccgt gccaggtgct
     3841 gcctcagatt caggttatgc cgctcaattc gctgcgtata tcgcttgctg attacgtgca
     3901 gctttccctt caggcgggat tcatacagcg gccagccatc cgtcatccat atcaccacgt
     3961 caaagggtga cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata
     4021 cgtgcgcaac aaccgtcttc cggagactgt catacgcgta aaacagccag cgctggcgcg
     4081 atttagcccc gacatagccc cactgttcgt ccatttccgc gcagacgatg acgtcactgc
     4141 ccggctgtat gcgcgaggtt accgactgcg gcctgagttt tttaagtgac gtaaaatcgt
     4201 gttgaggcca acgcccataa tgcgggctgt tgcccggcat ccaacgccat tcatggccat
     4261 atcaatgatt ttctggtgcg taccgggttg agaagcggtg taagtgaact gcagttgcca
     4321 tgttttacgg cagtgagagc agagatagcg ctgatgtccg gcggtgcttt tgccgttacg
     4381 caccaccccg tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc
     4441 acctcaaaaa caccatcata cactaaatca gtaagttggc agcatcaccc ctggttggct
     4501 tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc
     4561 gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac
     4621 acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc
     4681 aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat
     4741 ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa aagatccgtc
     4801 gacgatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta
     4861 tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag
     4921 cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca
     4981 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     5041 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     5101 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     5161 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     5221 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     5281 taatgtactg aattaattcg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     5341 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     5401 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     5461 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     5521 aagaaacttt attgccaaat gtttgaacga tctgcttcga cgcactcctt ctttactcca
     5581 ccatctcgtc cttattgaaa acgtgggtag caccaaaacg aatcaagtcg ctggaactga
     5641 agttaccaat cacgctggat gatttgccag ttggattaat cttgcctttc cccgcatgaa
     5701 taatattgat gaatgcatgc gtgaggggta tttcgatttt ggcaatagct gcaattgccg
     5761 cgacatcctc caacgagcat aattcttcag aaaaatagcg atgttccatg ttgtcagggc
     5821 atgcatgatg cacgttatga ggtgacggtg ctaggcagta ttccctcaaa gtttcatagt
     5881 cagtatcata ttcatcattg cattcctgca agagagaatt gagacgcaat ccacacgctg
     5941 cggcaacctt ccggcgttcg tggtctattt gctcttggac gttgcaaacg taagtgttgg
     6001 atcggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     6061 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     6121 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     6181 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     6241 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     6301 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     6361 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     6421 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     6481 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     6541 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     6601 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     6661 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     6721 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     6781 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     6841 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     6901 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcccg gatccgggcg
     6961 gaaataggta aagaagttgc ggataaggta attgccattg cagattattt ggattgagag
     7021 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt
     7081 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg
     7141 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccattaacgc ttacaatttc
     7201 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     7261 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     7321 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     7381 ggcgaattgg gcccctgcag gtcaacatgg tggagcacga cactctcgtc tactccaaga
     7441 atatcaaaga tacagtctca gaagaccaga gggctattga gacttttcaa caaagggtaa
     7501 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
     7561 tagaaaagga agatggcttc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
     7621 aagatgcctc taccgacagt ggtcccaaag atggaccccc acccacgagg aacatcgtgg
     7681 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     7741 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     7801 gttcatttca tttggagagg acctcgagaa caatggctag tgaagctcga aaaacaaaga
     7861 aaaaaatcaa agggattcag caagccactg caggagtctc acaagacact tcggaaaatc
     7921 ctaacaaaac aatagttcct gcagcattac cacagctcac ccctaccttg gtgtcactgc
     7981 tggaggtgat tgaacccgag gtgttgtatg caggatatga tagctctgtt ccagattcag
     8041 catggagaat tatgaccaca ctcaacatgt taggtgggcg tcaagtgatt gcagcagtga
     8101 aatgggcaaa ggcgatacca ggcttcagaa acttacacct ggatgaccaa atgaccctgc
     8161 tacagtactc atggatgttt ctcatggcat ttgccctggg ttggagatca tacagacaat
     8221 caagtggaaa cctgctctgc tttgctcctg atctgattat taatgagcag agaatgtctc
     8281 taccctgcat gtatgaccaa tgtaaacaca tgctgtttgt ctcctctgaa ttacaaagat
     8341 tgcaggtatc ctatgaagag tatctctgta tgaaaacctt actgcttctc tcctcagttc
     8401 ctaaggaagg tctgaagagc caagagttat ttgatgagat tcgaatgact tatatcaaag
     8461 agctaggaaa agccatcgtc aaaagggaag ggaactccag tcagaactgg caacggtttt
     8521 accaactgac aaagcttctg gactccatgc atgaggtggt tgagaatctc cttacctact
     8581 gcttccagac atttttggat aagaccatga gtattgaatt cccagagatg ttagctgaaa
     8641 tcatcactaa tcagatacca aaatattcaa atggaaatat caaaaagctt ctgtttcatc
     8701 aaaaatctac tagcaaaccg gtaacgttat acgacgtcgc tgaatacgcc ggcgtttctc
     8761 atcaaaccgt ttctagagtg gttaaccagg cttcacatgt tagcgctaaa acccgggaaa
     8821 aagttgaagc tgccatggct gagctcaact acatcccgaa ccgtgttgcg cagcagctgg
     8881 ctggtaaaca aagcttgctg atcggtgtcg cgacctcgag cttggccctg cacgcgccgt
     8941 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     9001 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     9061 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     9121 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     9181 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
     9241 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     9301 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     9361 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     9421 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     9481 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     9541 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     9601 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     9661 agctgttgcc cgtctcactg gtgaaaagaa aaaccactag tggatcggaa ttcgccaatt
     9721 ttaatcaaag tgggaatatt gctgatagct cattgtcctt cactttcact aacagtagca
     9781 acggtccgaa cctcataaca actcaaacaa attctcaagc gctttcacaa ccaattgcct
     9841 cctctaacgt tcatgataac ttcatgaata atgaaatcac ggctagtaaa attgatgatg
     9901 gtaataattc aaaaccactg tcacctggtt ggacggacca aactgcgtat aacgcgtttg
     9961 gaatcactac agggatgttt aataccacta caatggatga tgtatataac tatctattcg
    10021 atgatgaaga taccccacca aacccaaaaa aagagtaaaa tgaatcgtag atactgaaaa
    10081 accccgcaag ttcacttcaa ctgtgcatcg tgcaccatct caatttcttt catttataca
    10141 tcgttttgcc ttcttttatg taactatact cctctaagtt tcaatcttgg ccatgtaacc
    10201 tctgatctat agaatttttt aaatgactag aattaatgcc catctttttt ttggacctaa
    10261 attcttcatg aaaatatatt acgagggctt attcagaagc tggggatcct ctagagtccg
    10321 caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt
    10381 gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc
    10441 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
    10501 aattcctaaa accaaaatcc agtgacctgc aggggcccga cgtcgcatgc tcccggccgc
    10561 catggccgcg ggatatcact agtgcggccg ctcgacgaat taattccaat cccacaaaaa
    10621 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
    10681 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
    10741 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
    10801 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
    10861 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
    10921 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
    10981 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
    11041 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
    11101 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggctggcgaa
    11161 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    11221 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtat
    11281 cgacggtatc gataagctgg tcactggatt ttggttttag gaattagaaa ttttattgat
    11341 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
    11401 gaaaccctat aagaacccta attcccttat ctgggaacta ctcacacatt attctggaga
    11461 aaaatagaga gagatagatt tgtagagaga gactggtgat ttttgcggac tcccgaggct
    11521 gtagccgacg atggtgcgcc aggagagttg ttgattcatt gtttgcctcc ctgctgcggt
    11581 ttttcaccga agttcatgcc agtccagcgt ttttgcagca gaaaagccgc cgacttcggt
    11641 ttgcggtcgc gagtgaagat ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag
    11701 gtcgcaaaat cggcgaaatt ccatacctgt tcaccgacga cggcgctgac gcgatcaaag
    11761 acgcggtgat acatatccag ccatgcacac tgatactctt cactccacat gtcggtgtac
    11821 attgagtgca gcccggctaa cgtatccacg ccgtattcgg tgatgataat cggctgatgc
    11881 agtttctcct gccaggccag aagttctttt tccagtacct tctctgccgt ttccaaatcg
    11941 ccgctttgga cataccatcc gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg
    12001 atggtatcgg tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg acgcgtcggg
    12061 tcgagtttac gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg
    12121 gtatccggtt cgttggcaat actccacatc accacgcttg ggtggttttt gtcacgcgct
    12181 atcagctctt taatcgcctg taagtgcgct tgctgagttt ccccgttgac tgcctcttcg
    12241 ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag
    12301 ccgacagcag cagtttcatc aatcaccacg atgccatgtt catctgccca gtcgagcatc
    12361 tcttcagcgt aagggtaatg cgaggtacgg taggagttgg ccccaatcca gtccattaat
    12421 gcgtggtcgt gcaccatcag cacgttatcg aatcctttgc cacgcaagtc cgcatcttca
    12481 tgacgaccaa agccagtaaa gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc
    12541 actgccactg accggatgcc gacgcgaagc gggtagatat cacactctgt ctggcttttg
    12601 gctgtgacgc acagttcata gagataacct tcacccggtt gccagaggtg cggattcacc
    12661 acttgcaaag tcccgctagt gccttgtcca gttgcaacca cctgttgatc cgcatcacgc
    12721 agttcaacgc tgacatcacc attggccacc acctgccagt caacagacgc gtggttacag
    12781 tcttgcgcga catgcgtcac cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag
    12841 agcattacgc tgcgatggat tccggcatag ttaaagaaat cacggaagta agactgcttt
    12901 ttcttgccgt tttcgtcggt aatcaccatt cccggcggga tagtctgcca gttcagttcg
    12961 ttgttcacac aaacggtgat acgtacactt ttcccggcaa taacatacgg cgtgacatcg
    13021 gcttcaaatg gcgtatagcc gccctgatgc tccatcactt cctgattatt gacccacact
    13081 ttgccgtaat gagtgaccgc atcgaaacgc agcacgatac gctggcctgc ccaacctttc
    13141 ggtataaaga cttcgcgctg ataccagacg ttgcccgcat aattacgaat atctgcatcg
    13201 gcgaactgat cgttaaaact gcctggcaca gcaattgccc ggctttcttg taacgcgctt
    13261 tcccaccaac gctgatcaat tccacagttt tcgcgatcca gactgaatgc ccacaggccg
    13321 tcgagttttt tgatttcacg ggttggggtt tctacaggac ggaccatggt ggccactcga
    13381 ctctagctgt agttgtagaa tgtaaaatgt aatgttgttg ttgtttgttg ttgttgttgg
    13441 taattgttgt aaaaatacgc gcgtctagct tcagcgtgtc ctctccaaat gaaatgaact
    13501 tccttatata gaggaagggt cttgcgaaga tcgatccact agagcggccg gccgccaccg
    13561 cggtggagct agaaagaaga aagggaagag aaagaattgt gagcgctcac aattgaaaga
    13621 ctagaaagaa gaaagggaag agaaagaatt gtgagcgctc acaattgaaa gactagaaag
    13681 aagaaaggga agagaaagaa ttgtgagcgc tcacaattga aagactagaa agaagaaagg
    13741 gaagagaaag aattgtgagc gctcacaatt gaaagactag aaagaagaaa gggaagagaa
    13801 agaattgtga gcgctcacaa ttgaaagact agaaagaaga aagggaagag aaagaattgt
    13861 gagcgctcac aattgaaaga ctagtggatc gatcttcgca agacccttcc tctatataag
    13921 gaagttcatt tcatttggag aggacacgct gaagctagac gcgcgtattt ttacaacaat
    13981 taccaacaac aacaacaaac aacaacaaca ttacatttta cattctacaa ctacagctag
    14041 caagcttgtc gacctcgaga caagtttgta caaaaaagca ggctccgcgg ccgccccctt
    14101 caccatggct gataataagg tcaatctttc gattaatgga caatcaaaag tgcctccagg
    14161 tttcagattc catcccaccg aagaagaact tctccattac tatctccgta agaaagttaa
    14221 ctctcaaaag atcgatcttg atgtcattcg tgaagttgat ctaaacaagc ttgagccttg
    14281 ggatattcaa gaggaatgta gaatcggttc aacgccacaa aacgactggt acttcttcag
    14341 ccacaaggac aagaagtatc caaccgggac caggacgaac cgggcaacag tcgctggatt
    14401 ctggaaagct accggacgtg acaaaatcat ctgcagttgt gtccggagaa ttggactgag
    14461 gaagacactc gtgttctaca aaggaagagc tcctcacggt cagaaatccg actggatcat
    14521 gcatgagtat cgcctcgacg atactccaat gtctaatggc tatgctgatg ttgttacaga
    14581 agatccaatg agctataacg aagaaggttg ggtggtatgt cgagtgttca ggaagaagaa
    14641 ctatcaaaag attgacgatt gtcctaaaat cactctatct tctttacctg atgacacgga
    14701 ggaagagaag gggcccacct ttcacaacac tcaaaacgtt accggtttag accatgttct
    14761 tctctacatg gaccgtaccg gttctaacat ttgcatgccc gagagccaaa caacgactca
    14821 acatcaagat gatgtcttat tcatgcaact cccaagtctt gagacaccta aatccgagag
    14881 cccggtcgac caaagtttcc tgactccaag caaactcgat ttctctcccg ttcaagagaa
    14941 gataaccgaa agaccggttt gcagcaactg ggctagtctt gaccggctcg tagcttggca
    15001 attgaacaat ggtcatcata atccgtgtca tcgtaagagt tttgatgaag aagaagaaaa
    15061 tggtgatact atgatgcagc gatgggatct tcattggaat aatgatgata atgttgatct
    15121 ttggagtagt ttcactgagt cttcttcgtc tttagaccca cttcttcatt tatctgtatg
    15181 aaagggtggg cgcgccgacc cagctttctt gtacaaagtg gtctcgatcg aggaattcgg
    15241 tacccgggga tccactagag tcctgcttta atgagatatg cgagacgcct atgatcgcat
    15301 gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga
    15361 tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt
    15421 atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt
    15481 aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca
    15541 caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc
    15601 ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg
    15661 ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt
    15721 ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg
    15781 aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc
    15841 ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac
    15901 cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga
    15961 ggctcagcag gaccggcatg caagctagct tactagtgat attctatagt gtcacctaaa
    16021 tctgcggccg ccaccgcggt ggagaaacta tcagtgtttg acaggatata ttggcgggta
    16081 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
    16141 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggagtgcttg
    16201 gcattccgtg cgataatgac ttctgttcaa ccacccaaac gtcggaaagc ctgacgacgg
    16261 agcagcattc caaaaagatc ccttggctcg tctgggtcgg ctagaaggtc gagtgggctg
    16321 ctgtggcttg atccctcaac gcggtcgcgg acgtagcgca gcgccgaaaa atcctcgatc
    16381 gcaaatccga cgctgtcgaa aagcgtgatc tgcttgtcgc tctttcggcc gacgtcctgg
    16441 ccagtcatca cgcgccaaag ttccgtcaca ggatgatctg gcgcgagttg ctggatctcg
    16501 ccttcaatcc gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag caagatcgtc
    16561 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
    16621 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccggccgc
    16681 cggcgttgtg gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt
    16741 gacacttgag gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt
    16801 cggccggcga cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg
    16861 agtttcccac agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg
    16921 aggggcgcga ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct
    16981 gacagatgag gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc
    17041 atttgcaagg gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt
    17101 aaaccaatat ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc
    17161 acgccgaagg ggggtgcccc cccttctcga accctcccgg cccgctaacg cgggcctccc
    17221 atccccccag gggctgcgcc cctcggccgc gaacggcctc accccaaaaa tggcagccaa
    17281 gctcctaaca ttttattaga gagcaggcta gttgcttaga tacatgatct tcaggccgtt
    17341 atctgtcagg gcaagcgaaa attggccatt tatgacgacc aatgccccgc agaagctccc
    17401 atctttgccg ccatagacgc cgcgcccccc ttttggggtg tagaacatcc ttttgccaga
    17461 tgtggaaaag aagttcgttg tcccattgtt ggcaatgacg tagtagccgg cgaaagtgcg
    17521 agacccattt gcgctatata taagcctacg atttccgttg cgactattgt cgtaattgga
    17581 tgaactatta tcgtagttgc tctcagagtt gtcgtaattt gatggactat tgtcgtaatt
    17641 gcttatggag ttgtcgtagt tgcttggaga aatgtcgtag ttggatgggg agtagtcata
    17701 gggaagacga gcttcatcca ctaaaacaat tggcaggtca gcaagtgcct gccccgatgc
    17761 catcgcaagt acgaggctta gaaccacctt caacagatcg cgcatagtct tccccagctc
    17821 tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa ttgttagaca
    17881 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tgaacaaatt cttccaactg
    17941 atctgcgcgc gaggccaagc gatcttcttg tccaagataa gcctgcctag cttcaagtat
    18001 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
    18061 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
    18121 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
    18181 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
    18241 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
    18301 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
    18361 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
    18421 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
    18481 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
    18541 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
    18601 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
    18661 ttccctcatg atgtttaact cctgaattaa gccgcgccgc gaagcggtgt cggcttgaat
    18721 gaattgttag gcgtcatcct gtgctcccga gaaccagtac cagtacatcg ctgtttcgtt
    18781 cgagacttga ggtctagttt tatacgtgaa caggtcaatg ccgccgagag taaagccaca
    18841 ttttgcgtac aaattgcagg caggtacatt gttcgtttgt gtctctaatc gtatgccaag
    18901 gagctgtctg cttagtgccc actttttcgc aaattcgatg agactgtgcg cgactccttt
    18961 gcctcggtgc gtgtgcgaca caacaatgtg ttcgatagag gctagatcgt tccatgttga
    19021 gttgagttca atcttcccga caagctcttg gtcgatgaat gcgccatagc aagcagagtc
    19081 ttcatcagag tcatcatccg agatgtaatc cttccggtag gggctcacac ttctggtaga
    19141 tagttcaaag ccttggtcgg ataggtgcac atcgaacact tcacgaacaa tgaaatggtt
    19201 ctcagcatcc aatgtttccg ccacctgctc agggatcacc gaaatcttca tatgacgcct
    19261 aacgcctggc acagcggatc gcaaacctgg cgcggctttt ggcacaaaag gcgtgacagg
    19321 tttgcgaatc cgttgctgcc acttgtttaa tagactggat ggaggcggat aaagttgcag
    19381 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    19441 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    19501 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    19561 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    19621 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    19681 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    19741 ccgtagaaaa gatcaaagga tcttc
//
