LOCUS       sequence-456850-        5038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of a de novo designed, FRET-based 
            neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh 
            AH-W
  TITLE     De novo luciferases enable multiplexed bioluminescence imaging.
  JOURNAL   Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. 
            Epub 2024 Nov 12.
  PUBMED    40417169
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem."; 
            date: "2025-03-13"; volume: "11(3):102346. doi"; pages: " 
            10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12"
COMMENT     Sequence Label: pBAD-luxOFP
FEATURES             Location/Qualifiers
     source          1..5038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        649..933
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     856..875
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2029..2046
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2087..2104)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2257..2343
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2435..2462
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2481..2572
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2573..2641,2642..3433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2791..2810)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3604..4192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4093..4112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4346..4363
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4378..4518
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4444..4463)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4604..4626
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(4782..5038,1..622))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
       61 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
      121 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
      181 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
      241 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
      301 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
      361 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
      421 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
      481 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
      541 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
      601 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
      661 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca
      721 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
      781 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
      841 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
      901 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
      961 ttaaccatgg gctcaggaac agacgaagaa atagcagaat tcgtaaaagc attctacgaa
     1021 gcactcgacg caggagacgc agaaacagca gcagacctac tattcggagc aggatgcaaa
     1081 gaaatacacc tatgggacgg aacagtattc acaagcaaag aaggattcga aacatggttc
     1141 aaaaaactac aaagcgcagt aacaggagca aaaagaaaaa tagaaagcgt agaaataaaa
     1201 gacggagaag cagtagtaaa actaacacta acagcaacat acaaagcaac aggaaaaaaa
     1261 ttcgtagtag aactagaaca cacattcaca ttcgacagag aaagaaacga actagtagaa
     1321 gtgaaagtca cagcaacacc gtatattaaa gaaaacatgc gtagcaaact gtatctggag
     1381 ggctctgtga acggtcatca gtttaaatgc acccatgaag gtgaaggcaa accgtatgaa
     1441 gggaaacaga ctaaccgcat taaagtggtt gaaggcggtc cgctgccgtt tgcgtttgat
     1501 attctggcga cccattttat gtatggcagc aaagtgttta ttaaatatcc ggcggatctg
     1561 ccggattatt ttaaacagtc gtttccggaa ggttttacct gggaacgcgt gatggtgttt
     1621 gaagatggtg gcgtgctgac ggcgactcag gataccagcc tgcaggatgg tgaactgatt
     1681 tataacgtca aagtgcgcgg ggttaacttt ccagcgaacg gcccggtgat gcagaaaaag
     1741 accttaggtt gggaaccgag caccgaaacc atgtatccag cggatggcgg tctggaaggc
     1801 cgctgcgata aagccctgaa attagtgggc ggcggccatc tgcatgtgaa ctttaaaacc
     1861 acgtacaaaa gcaaaaagcc ggtgaaaatg ccgggcgtgc attacgtgga tcgccgttta
     1921 gaacgcataa aagaagcgga taacgaaacc tatgttgaac agtatgaaca tgcagtggcg
     1981 cgctatagca acttaggggg cggcatggat gaactgtaca aaggttctca tcatcatcat
     2041 catcattaaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
     2101 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
     2161 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
     2221 agtaagccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     2281 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     2341 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
     2401 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
     2461 tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct
     2521 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2581 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2641 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2701 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2761 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     2821 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2881 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2941 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3001 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3061 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3121 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3181 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3241 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggta gtcgcggtat
     3301 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3361 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3421 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3481 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3541 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3721 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4201 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4261 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4321 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     4381 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     4441 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     4501 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4561 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4621 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc gcgcgcgaag
     4681 gcgaagcggc atgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta
     4741 tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct
     4801 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat
     4861 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg
     4921 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg
     4981 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacg
//
