LOCUS       sequence-456853-       11449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is a lentiviral reporter for translation initiation under the 
            control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). 
            There is a control destabilized mCherry (dsmCherry) as a control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Kovalski JR, Sarioglu G, Subramanyam V, Hernandez G, Rademaker G, 
            Oses-Prieto JA, Slota M, Mohan N, Yiakis K, Liu I, Wen KW, Kim GE, 
            Miglani S, Burlingame AL, Goodarzi H, Perera RM, Ruggero D
  TITLE     Functional screen identifies RBM42 as a mediator of oncogenic mRNA 
            translation specificity.
  JOURNAL   Nat Cell Biol. 2025 Feb 4. doi: 10.1038/s41556-024-01604-7.
  PUBMED    39905246
REFERENCE   2  (bases 1 to 11449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 Feb 4. doi: 10.1038/s41556-024-01604-7."
COMMENT     Sequence Label: Bidirectional MYC 5'UTR Dual Fluorescence Repo...
FEATURES             Location/Qualifiers
     source          1..11449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    2184..2408
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2556..3263)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2831..2850)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3100..3118
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(3273..3783)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3336..3354)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        3842..4053
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4054..4992
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4949..4969
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(5448..5450,5451..5453,5454..6164,6165..6170,
                     6171..6293)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(5493..5514)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5754..5773)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6101..6122
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6413..6962
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6557..6574)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6784..6803
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    7417..8005
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7470..7490)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7888..7899)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8212..8845
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8973..8989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8973..8989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8986..9008)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8997..9013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9021..9027,9028..9045,9046..9051))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9066..9087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9204..9221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9375..9963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9455..9474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10134..10925,10926..10994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10757..10776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10995..11099)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11147..11281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11184..11203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11238..11257
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11449,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatccataga gcccaccgca tccccagcat gcctgctatt
     2221 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
     2281 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
     2341 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
     2401 aggcacagtc ggtcgacgcg gccgctctac acattgatcc tagcagaagc acaggctgca
     2461 gggtgacggt ccatcccgct ctcctgggca caagacatgg gcagcgtgcc atcatcctgc
     2521 tcctccacct ccggcgggaa gccatggcta agcttcttgt acagctcgtc catgccgccg
     2581 gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg
     2641 gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg
     2701 gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc
     2761 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
     2821 cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
     2881 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
     2941 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
     3001 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
     3061 ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg
     3121 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
     3181 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
     3241 tcctcctcgc ccttgctcac catcggatcc ccctggggag agaggtcggt gattcggtca
     3301 acgagggagc cgactgccga cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg
     3361 ggcaggaaca gggcccacac taccgcccca caccccgcct cccgcaccgc cccttcccgg
     3421 ccgctgctct cggcgcgccc tgctgagcag ccgctattgg ccacagccca tcgcggtcgg
     3481 cgcgctgcca ttgctccctg gcgctgtccg tctgcgaggg tactagtgag acgtgcggct
     3541 tccgtttgtc acgtccggca cgccgcgaac cgcaaggaac cttcccgact taggggcgga
     3601 gcaggaagcg tcgccggggg gcccacaagg gtagcggcga agatccgggt gacgctgcga
     3661 acggacgtga agaatgtgcg agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc
     3721 ccagagcagc cgcgtccctg cgcaaaccca gggctgcctt ggaaaaggcg caaccccaac
     3781 cccgtggcaa ttcgatatca tcgatgaggc cctttcgtct tcactcgagg tgcccgtcag
     3841 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     3901 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     3961 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     4021 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     4081 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     4141 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     4201 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     4261 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     4321 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     4381 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     4441 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     4501 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     4561 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     4621 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     4681 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     4741 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     4801 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     4861 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     4921 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     4981 cttccatttc aggtgtcgtg aggatccgcg gccgcgccgg ctctagatcg cgaacgcgta
     5041 actcgctgta gtaattccag cgagaggcag agggagcgag cgggcggccg gctagggtgg
     5101 aagagccggg cgagcagagc tgcgctgcgg gcgtcctggg aagggagatc cggagcgaat
     5161 agggggcttc gcctctggcc cagccctccc gctgatcccc cagccagcgg tccgcaaccc
     5221 ttgccgcatc cacgaaactt tgcccatagc agcgggcggg cactttgcac tggaacttac
     5281 aacacccgag caaggacgcg actctcccga cgcggggagg ctattctgcc catttgggga
     5341 cacttccccg ccgctgccag gacccgcttc tctgaaaggc tctccttgca gctgcttaga
     5401 cgctggattt ttttcgggta gtggaaaacc agcagcctcc cgcgacgatg gtgagcaagg
     5461 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     5521 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     5581 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     5641 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     5701 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     5761 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     5821 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5881 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     5941 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     6001 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     6061 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     6121 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaagctt agccatggct
     6181 tcccgccgga ggtggaggag caggatgatg gcacgctgcc catgtcttgt gcccaggaga
     6241 gcgggatgga ccgtcaccct gcagcctgtg cttctgctag gatcaatgtg tagacgcgtg
     6301 aattcctcga gggcggccgc tctagagtcg acgggccgcg gtaacaattg ttaactaact
     6361 taagctagca acggtttccc tctagcggga tcaattccgc cccccccccc taacgttact
     6421 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     6481 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6541 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6601 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     6661 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     6721 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6781 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6841 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6901 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa
     6961 taccatggtc atgaaaacat ttaacatttc tcaacaagat ctagaattag tagaagtagc
     7021 gacagagaag attacaatgc tttatgagga taataaacat catgtgggag cggcaattcg
     7081 tacgaaaaca ggagaaatca tttcggcagt acatattgaa gcgtatatag gacgagtaac
     7141 tgtttgtgca gaagccattg cgattggtag tgcagtttcg aatggacaaa aggattttga
     7201 cacgattgta gctgttagac acccttattc tgacgaagta gatagaagta ttcgagtggt
     7261 aagtccttgt ggtatgtgta gggagttgat ttcagactat gcaccagatt gttttgtgtt
     7321 aatagaaatg aatggcaagt tagtcaaaac tacgattgaa gaactcattc cactcaaata
     7381 tacccgaaat taacccgggc ggggcgcgtc tggaacaatc aacctctgga ttacaaaatt
     7441 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7501 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7561 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7621 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7681 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7741 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7801 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7861 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7921 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7981 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     8041 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     8101 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     8161 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     8221 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     8281 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     8341 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     8401 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     8461 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     8521 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     8581 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     8641 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     8701 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8761 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8821 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8881 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg
     8941 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9001 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9061 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9121 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9181 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9241 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9301 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9361 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9421 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9481 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9541 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9601 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9661 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9721 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9781 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9841 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9901 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9961 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10021 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10081 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10141 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10201 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10261 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10321 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10381 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10441 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10501 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10561 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10621 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10681 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10741 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10801 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10861 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10921 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10981 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11041 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11101 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
    11161 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11221 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    11281 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    11341 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    11401 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagtt
//
