LOCUS       sequence-456858-        9448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP and scramble of shRNA targeting Atg7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Wang S, Baumert R, Sejourne G, Bindu DS, Dimond K, Sakers K, Vazquez
            L, Moore JL, Tan CX, Takano T, Rodriguez MP, Brose N, Bradley L, 
            Lessing R, Soderling SH, La Spada AR, Eroglu C
  TITLE     PD-linked LRRK2 G2019S mutation impairs astrocyte morphology and 
            synapse maintenance via ERM hyperphosphorylation
  JOURNAL   eLife 2025 [Reviewed Preprint] 14:RP107556. doi: 
            https://doi.org/10.7554/eLife.107556.1
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife 2025 
            [Reviewed Preprint] 14:RP107556. doi: 
            https://doi.org/10.7554/eLife.107556.1"
COMMENT     Sequence Label: pLKO.1-shControl-EGFP
FEATURES             Location/Qualifiers
     source          1..9448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             84..128
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        426..674
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     426..446
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     597..616
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    771..888
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1255..1558
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1560..1835
                     /label=chicken beta-actin promoter
     intron          1836..2852
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2776..2798
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2860..2879
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2929..2938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2935..2937,2938..2940,2941..3654)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2935..2937,2938..2940,2941..3651)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2980..3001)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3241..3260)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3588..3609
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3674..3707)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3763..4351
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3816..3836)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4234..4245)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4354..4370)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4355..4371)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4560..4793
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4865..4986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4902..4921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4956..4975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5026..5161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5181..5200)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5182..5200)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5210..5227)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5210..5226)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5219..5241)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5368..5823
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5455..5474)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5665..5686
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5849..5953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5954..6022,6023..6814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6172..6191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6985..7573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7474..7493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7727..7744
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7861..7882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7897..7902,7903..7920,7921..7927)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7935..7951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7940..7962
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7959..7975
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7959..7975
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7994..8014
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7996..8014
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8042..8268
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8269..8449
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8496..8621
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9114..9347
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
       61 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
      121 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
      181 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
      241 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      301 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      361 gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg gccgccccct
      421 tcaccgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
      481 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
      541 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      601 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      661 aaggacgaaa caccggagta ctattcataa ccgcaacctc gaggttgcgg ttatgaatag
      721 tacttttttg aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
      781 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      841 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      901 agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca
      961 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
     1021 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
     1081 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
     1141 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtta
     1201 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     1261 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     1321 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     1381 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     1441 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     1501 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     1561 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     1621 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
     1681 gcgcgccagg gggggggggg gggggggagg gggggggsgg ggsgaggcgg agaggtgcgg
     1741 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     1801 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
     1861 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
     1921 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
     1981 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
     2041 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
     2101 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     2161 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     2221 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     2281 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     2341 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     2401 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggrgggct
     2461 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     2521 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     2581 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     2641 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     2701 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     2761 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     2821 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     2881 tgtctcatca ttttggcaaa gaattcgcgg ccgctagcgc taccggtcgc caccatggtg
     2941 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3001 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3061 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3121 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3181 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3241 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3301 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3361 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3421 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3481 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3541 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3601 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaggaattc
     3661 gtcgagggac ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg
     3721 ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac
     3781 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3841 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3901 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3961 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4021 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4081 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4141 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4201 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4261 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4321 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     4381 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     4441 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     4501 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4561 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     4621 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4681 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4741 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     4801 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     4861 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4921 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4981 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     5041 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5101 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5161 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     5221 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     5281 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5341 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     5401 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5461 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5521 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5581 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5641 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5701 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5761 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5821 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5881 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5941 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6001 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6061 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6121 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6181 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6241 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6301 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6361 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6421 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6481 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6541 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6601 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6661 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6721 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6781 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6841 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6901 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6961 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7021 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7081 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7141 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7201 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7261 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7321 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7381 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7441 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7501 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7561 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7621 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7681 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7741 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7801 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7861 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7921 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7981 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     8041 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     8101 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     8161 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     8221 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     8281 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8341 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8401 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8461 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8521 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8581 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8641 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8701 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8761 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8821 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8881 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8941 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     9001 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9061 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9121 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9181 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9241 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9301 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9361 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9421 tctctggaac agatttggaa tcacacga
//
