LOCUS       sequence-456862-        9449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP and shRNA targeting Atg7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Wang S, Baumert R, Sejourne G, Bindu DS, Dimond K, Sakers K, Vazquez
            L, Moore JL, Tan CX, Takano T, Rodriguez MP, Brose N, Bradley L, 
            Lessing R, Soderling SH, La Spada AR, Eroglu C
  TITLE     PD-linked LRRK2 G2019S mutation impairs astrocyte morphology and 
            synapse maintenance via ERM hyperphosphorylation
  JOURNAL   eLife 2025 [Reviewed Preprint] 14:RP107556. doi: 
            https://doi.org/10.7554/eLife.107556.1
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife 2025 
            [Reviewed Preprint] 14:RP107556. doi: 
            https://doi.org/10.7554/eLife.107556.1"
COMMENT     Sequence Label: pLKO.1-shAtg7-EGFP
FEATURES             Location/Qualifiers
     source          1..9449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     277..298
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(363..396)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    452..1040
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(505..525)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(923..934)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1043..1059)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1044..1060)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1249..1482
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1554..1675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1591..1610)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1645..1664
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1715..1850
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1870..1889)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1871..1889)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1899..1916)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1899..1915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1908..1930)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2057..2512
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2144..2163)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2354..2375
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2538..2642
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2643..2711,2712..3503)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2861..2880)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3674..4262
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4163..4182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4416..4433
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4550..4571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4586..4591,4592..4609,4610..4616)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4624..4640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4629..4651
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4648..4664
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4648..4664
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4683..4703
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4685..4703
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4731..4957
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4958..5138
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5185..5310
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5803..6036
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6221..6265
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6563..6811
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6563..6583
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6734..6753
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    6908..7025
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7392..7695
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7697..7973
                     /label=chicken beta-actin promoter
     intron          7974..8990
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8914..8936
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     8998..9017
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      9067..9076
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9073..9075,9076..9078,9079..9449,1..343)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9073..9075,9076..9078,9079..9449,1..340)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9118..9139)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9379..9398)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
       61 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      121 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      181 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
      241 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      301 cgccgccggg atcactctcg gcatggacga gctgtacaag taggaattcg tcgagggacc
      361 taataacttc gtatagcata cattatacga agttatacat gtttaagggt tccggttcca
      421 ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
      481 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
      541 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
      601 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
      661 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
      721 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
      781 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
      841 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
      901 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
      961 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     1021 cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta gaaaaacatg
     1081 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     1141 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     1201 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     1261 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     1321 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     1381 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     1441 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     1501 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     1561 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1621 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1681 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1741 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1801 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     1861 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     1921 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     1981 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2041 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2101 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2161 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2221 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     2281 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2341 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     2401 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     2461 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     2521 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2581 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2641 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2701 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2761 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2821 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2881 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2941 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3001 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3061 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3121 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3181 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3241 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3301 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3361 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3421 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3481 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3541 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3601 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3661 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3721 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3781 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3841 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3901 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3961 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4021 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4081 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4141 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4201 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4261 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4321 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4381 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4441 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4501 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     4561 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     4621 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     4681 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     4741 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     4801 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     4861 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     4921 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     4981 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5041 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5101 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     5161 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     5221 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     5281 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     5341 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     5401 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     5461 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     5521 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     5581 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     5641 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     5701 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     5761 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     5821 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     5881 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     5941 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     6001 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     6061 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     6121 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     6181 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     6241 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     6301 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     6361 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     6421 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     6481 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac gagactagcc
     6541 tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat atttgcatat
     6601 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
     6661 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
     6721 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
     6781 tttatatatc ttgtggaaag gacgaaacac cggccagctc tgaactcaat aatactcgag
     6841 tattattgag ttcagagctg gtttttgaat tctcgacctc gagacaaatg gcagtattca
     6901 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     6961 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     7021 attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg
     7081 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     7141 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     7201 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     7261 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     7321 gcacgtctca ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     7381 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     7441 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     7501 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     7561 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     7621 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     7681 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     7741 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     7801 gcgggggggg gggggggsgc gcgccagggg gggggggggg gggggagggg gggggsgggg
     7861 sgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     7921 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     7981 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     8041 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     8101 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     8161 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     8221 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     8281 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     8341 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     8401 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     8461 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     8521 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     8581 ctcgggccgg ggrgggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     8641 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     8701 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     8761 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     8821 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     8881 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     8941 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     9001 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgcggcc gctagcgcta
     9061 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     9121 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     9181 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     9241 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     9301 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     9361 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     9421 ggcgacaccc tggtgaaccg catcgagct
//
