LOCUS       sequence-456886-       11406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control HA-tagged protein for immunoprecipitation experiments..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11406)
  AUTHORS   Kovalski JR, Sarioglu G, Subramanyam V, Hernandez G, Rademaker G, 
            Oses-Prieto JA, Slota M, Mohan N, Yiakis K, Liu I, Wen KW, Kim GE, 
            Miglani S, Burlingame AL, Goodarzi H, Perera RM, Ruggero D
  TITLE     Functional screen identifies RBM42 as a mediator of oncogenic mRNA 
            translation specificity.
  JOURNAL   Nat Cell Biol. 2025 Feb 4. doi: 10.1038/s41556-024-01604-7.
  PUBMED    39905246
REFERENCE   2  (bases 1 to 11406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 Feb 4. doi: 10.1038/s41556-024-01604-7."
COMMENT     Sequence Label: pLEX GFP-HA
FEATURES             Location/Qualifiers
     source          1..11406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    212..337
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             384..1017
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    1185..1319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1367..1471
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1472..1540,1541..2332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1690..1709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2503..3091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2992..3011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3245..3262
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3379..3400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3415..3420,3421..3438,3439..3445)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3453..3469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3458..3480
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3477..3493
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3477..3493
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3542..3663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3793..4818)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(3793..4818)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4867..5196)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4881..5016
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4935..4954)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5181..5201
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(5210..5638)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5320..5341)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5532..5551
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5684..5908
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5897..5914
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             6015..6248
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    6455..7043
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6561..6572
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6970..6990
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7050..7652)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7133..7153)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7655..8227
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7814..7833)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8043..8060
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(8270..8296)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(join(8324..9034,9035..9037,9038..9040))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8324..9034,9035..9037,9038..9040))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8366..8387)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8715..8734
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8974..8995
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9105..9129)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9126..9329)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9155..9175)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9330..9633
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9738..9785
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        9777..9795
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9799..9808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9805..10179
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    10244..10361
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10659..10703)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10888..11121
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
       61 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
      121 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
      181 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
      241 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
      301 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
      361 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
      421 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
      481 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
      541 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
      601 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
      661 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
      721 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
      781 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
      841 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
      901 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
      961 ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttccaact
     1021 actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag
     1081 gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat agggtatggg
     1141 gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca gacatgataa
     1201 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1261 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg
     1321 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1381 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1441 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1501 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1561 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1621 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1681 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1741 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1801 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1861 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1921 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1981 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     2041 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     2101 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2161 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     2221 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2281 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2341 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2401 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2461 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2521 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2581 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2641 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2701 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2761 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2821 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2881 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2941 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3001 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3061 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3121 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3181 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3241 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     3301 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     3361 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     3421 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     3481 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta tacagacatg
     3541 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3601 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3661 gttggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     3721 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     3781 tcgtagcacg tgctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
     3841 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
     3901 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
     3961 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
     4021 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
     4081 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
     4141 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
     4201 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
     4261 ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
     4321 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
     4381 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
     4441 atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc
     4501 ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag
     4561 gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa
     4621 gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac
     4681 atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg
     4741 catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt
     4801 gagttcaggc tttttcatca cgtgctgatc agatccgaaa atggatatac aagctcccgg
     4861 gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     4921 agaggcagag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     4981 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     5041 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     5101 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     5161 ggagcctggg gactttccac accctaactg acacacattc cacagaatta attcgcgtta
     5221 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     5281 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     5341 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     5401 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5461 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     5521 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     5581 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg
     5641 ggataccccc tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca
     5701 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca
     5761 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
     5821 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
     5881 acagatggct ggcaactaga aggcacagtc gaggctgatc agcgggttta aacaatgtca
     5941 aggcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
     6001 acatgaacta ctactgctag agattttcca cactgactaa aagggtctga gggatctcta
     6061 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
     6121 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
     6181 taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgaattag
     6241 cccttccagt cccctctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt
     6301 cattggtctt aaaggtacct gaggtgtgac tggaaaaccc acctcctcct cctcttgtgc
     6361 ttctagccag gcacaatcag cattggtagc tgctgtattg ctacttgtga ttgctccatg
     6421 tttttctagg tctcgactgc agaattaatt ccaggcgggg aggcggccca aagggagatc
     6481 cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg
     6541 cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc
     6601 agaatccagg tggcaacaca ggcgagcagc catggaaagg acgtcagctt ccccgacaac
     6661 accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg
     6721 gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga
     6781 caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg
     6841 ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac
     6901 aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca
     6961 gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa
     7021 attttgtaat ccagaggttg attgttaact caggcaccgg gcttgcgggt catgcaccag
     7081 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
     7141 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccagc gcgctcggcc
     7201 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     7261 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     7321 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgagccgatc
     7381 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
     7441 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     7501 tgcagctcgg tgacccgctc gatgtggcgg tccgggtcga cggtgtggcg cgtggcgggg
     7561 tagtcggcga acgcggcggc gagggtgcgt acggcccggg ggacgtcgtc gcgggtggcg
     7621 aggcgcaccg tgggcttgta ctcggtggcc atggtattat cgtgtttttc aaaggaaaac
     7681 cacgtccccg tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa
     7741 gcatgtgcac cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt
     7801 cagccccttg ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact
     7861 cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg
     7921 gccgcagagg cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga
     7981 cgttgtttgt cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc
     8041 ttgcattcct ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc
     8101 aggtttccgg gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac
     8161 aaacgcacac cggccttatt ccaagcggct tcggccagta acgttagggg ggggggaggg
     8221 agaggggcga ccggtggcca gacgcgtcgg gccctctaga ctcgagttaa gcatagtcag
     8281 gcacatcgta tgggtatcca ccgcctccct cgagcggccg caccttgtac agctcgtcca
     8341 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     8401 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
     8461 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     8521 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     8581 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     8641 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     8701 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     8761 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     8821 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     8881 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     8941 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     9001 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggatcctcta gtagagtcgg
     9061 tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
     9121 aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
     9181 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
     9241 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
     9301 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
     9361 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     9421 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     9481 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     9541 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     9601 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     9661 aatgaccccg taattgatta ctattaataa ctaatgcatg gcggtaatac gtctagaacc
     9721 tcggacctgc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     9781 acgactcact ataggagggc caccatggcc aagttgacca gtgccgttcc ggtgctcacc
     9841 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     9901 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     9961 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
    10021 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
    10081 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
    10141 ggcaactgcg tgcacttcgt ggccgaggag caggactgat tccggactag agcgggcccg
    10201 gtactaacca aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
    10261 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
    10321 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
    10381 tttgtctgca gaattggcgc acgcagtgcc gatccgttca ctaatcgaat ggatctgtct
    10441 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
    10501 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
    10561 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
    10621 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
    10681 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
    10741 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
    10801 atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
    10861 agttttccag agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca
    10921 gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt
    10981 tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt
    11041 ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag
    11101 aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc
    11161 tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat
    11221 tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag cggccggccg
    11281 cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct
    11341 tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata
    11401 atgatc
//
