LOCUS       sequence-456887-       12402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentiviral expression of Myc coding sequence with an HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12402)
  AUTHORS   Kovalski JR, Sarioglu G, Subramanyam V, Hernandez G, Rademaker G, 
            Oses-Prieto JA, Slota M, Mohan N, Yiakis K, Liu I, Wen KW, Kim GE, 
            Miglani S, Burlingame AL, Goodarzi H, Perera RM, Ruggero D
  TITLE     Functional screen identifies RBM42 as a mediator of oncogenic mRNA 
            translation specificity.
  JOURNAL   Nat Cell Biol. 2025 Feb 4. doi: 10.1038/s41556-024-01604-7.
  PUBMED    39905246
REFERENCE   2  (bases 1 to 12402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 Feb 4. doi: 10.1038/s41556-024-01604-7."
COMMENT     Sequence Label: pLEX Myc-HA
FEATURES             Location/Qualifiers
     source          1..12402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    687..821
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        869..973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(974..1042,1043..1834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1192..1211)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2005..2593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2494..2513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2747..2764
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2881..2902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2917..2922,2923..2940,2941..2947)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2955..2971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2960..2982
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2979..2995
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2979..2995
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3044..3165
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3295..4320)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(3295..4320)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4369..4698)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4383..4518
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4437..4456)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4683..4703
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4712..5140)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4822..4843)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5034..5053
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5186..5410
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5399..5416
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             5517..5750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5957..6545
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6063..6074
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6472..6492
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6552..7577)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    7580..8152
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7739..7758)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     7968..7985
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(8195..8221)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(8222..9538)
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     primer_bind     complement(9603..9627)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9624..9827)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9653..9673)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9828..10131
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10236..10283
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        10275..10293
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      10297..10306
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10303..10677
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    10742..10859
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(11157..11201)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11386..11619
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    12116..12241
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             join(12288..12402,1..519)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
       61 agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc
      121 cagtcccgcc caggccacgc ctccctggaa agtccccagc ggaaagtccc ttgtagcaag
      181 ctcgatatca gcagttcttg aagtactccg gatgcagctc tcgggccacg tgatgaaatg
      241 ctaggcggct gtcaaacctc cactctaaca cttctctctc cgggtcatcc atcccatgca
      301 ggctcacagg gtgtaacaag ctggtgttct ctcctttatt ggcctcttct accttatctg
      361 gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg acccctggcc
      421 ctggtgtgta gttctgctaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa
      481 ggatatcttg tcttctttgg gagtgaatta gcccttccaa ctactaagtt tgtagtacat
      541 atttaacaaa tacaatttct ttaaaatgaa aataattcag aggaatcaca ggtttagagt
      601 aaatgaaacc acaggtaatt ggcagtggta atagggtatg gggtgggaag tttgggatga
      661 ttttggttag cttgagttat ccagttgatc cagacatgat aagatacatt gatgagtttg
      721 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      781 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tgatctcccg atccgtcgac
      841 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      901 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      961 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1021 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1081 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1141 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1201 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1261 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1321 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1381 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1441 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1501 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1561 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1621 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1681 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1741 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1801 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1861 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1921 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1981 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2041 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2101 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2161 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2221 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2281 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2341 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2401 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2461 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2521 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2581 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2641 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2701 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2761 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     2821 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2881 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2941 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     3001 ttacgccaag ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag
     3061 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     3121 gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa
     3181 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     3241 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtagca cgtgctattc
     3301 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     3361 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     3421 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     3481 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg
     3541 cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     3601 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     3661 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     3721 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     3781 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     3841 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     3901 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     3961 atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg
     4021 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc
     4081 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     4141 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     4201 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     4261 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     4321 cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc
     4381 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc
     4441 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     4501 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     4561 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     4621 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     4681 acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc
     4741 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4801 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4861 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4921 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4981 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5041 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5101 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc
     5161 cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta
     5221 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     5281 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     5341 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     5401 gaaggcacag tcgaggctga tcagcgggtt taaacaatgt caaggcctct cactctctga
     5461 tattcatttc tttgcaagtt ataaatactg aataataaga tgacatgaac tactactgct
     5521 agagattttc cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa
     5581 cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg
     5641 gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt
     5701 acaagcaaaa agcagatctt gtcttcgttg ggagtgaatt agcccttcca gtcccctctt
     5761 ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc ttaaaggtac
     5821 ctgaggtgtg actggaaaac ccacctcctc ctcctcttgt gcttctagcc aggcacaatc
     5881 agcattggta gctgctgtat tgctacttgt gattgctcca tgtttttcta ggtctcgact
     5941 gcagaattaa ttccaggcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
     6001 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
     6061 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
     6121 caggcgagca gccatggaaa ggacgtcagc ttccccgaca acaccacgga attgtcagtg
     6181 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
     6241 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
     6301 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
     6361 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
     6421 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
     6481 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
     6541 tgattgttaa ctcattcctt ggctctgggt cttgtggagg gtctcctgtt tccagagtca
     6601 gccagaactt caacacatcc atcagtccaa acagcagcag accttcttgc aatttgagtt
     6661 cttccaacag ttccagcacc agacctcaca atggcatcac atcttccttg tgcccaagca
     6721 gcatcatcaa agtttccatc aaccagagat tgatacagtt ggtccaggcc aattctgagc
     6781 atgtaggctc tcagtctggg ggaaccagcc agttctgggt gtcttctttc aaaatatctt
     6841 gtttgttgtt ccatgcaagc cagccaaggt ctccaaaaaa aaatgttggc aacctcatat
     6901 tgagaatctc caaacatggc ttcagaccag tcaatgactg cagtgattct gccattgtct
     6961 gtcagaacat tgttgcttcc aaaatcagca tggaccaggt gtctgacttc aggacaatct
     7021 tctgcccaca gcatgagttc atccagtgcc tgagcaacag aagcagaaac tgtgtcatcc
     7081 atcacagtct gccagtgata gacatgagga tcagcaatgg cacaaatgaa atccctccaa
     7141 gtggtgtact gaccaatgcc ttggggacca aaaggaccaa atccagaggt ttggctcaga
     7201 tcagctgctg caatggcatc cattgcttca gcaacaggtt gcagaacagc tggcagctca
     7261 gtttcaggga gatcctggag agtgactcct tgtgctcttc tgctgatgca gtaggtgaga
     7321 gattcagaaa attctccaat gtccagaact tctggaattg gcagagcagc agaggcaaag
     7381 tgtctgtaaa catatctgtc tttgtaaaaa ccatcagcac aagaattgac cctcagaaca
     7441 taacctcttc ctccaacatc aaaagaaaag gctctgcttt cttcaccttc agacagctgc
     7501 atgagatcag aaacagaatc aaatttttca atgagaaact tctcaacaga agttgctgtc
     7561 agttcaggtt tcttcatggt attatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt
     7621 cggggggcct agacgttttt ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg
     7681 ccccagatca gatcccatac aatggggtac cttctgggca tccttcagcc ccttgttgaa
     7741 tacgcttgag gagagccatt tgactctttc cacaactatc caactcacaa cgtggcactg
     7801 gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc agaggcacct
     7861 gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg tttgtcttca
     7921 agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca ttcctttggc
     7981 gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt tccgggccct
     8041 cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg cacaccggcc
     8101 ttattccaag cggcttcggc cagtaacgtt aggggggggg gagggagagg ggcgaccggt
     8161 ggccagacgc gtcgggccct ctagactcga gttaagcata gtcaggcaca tcgtatgggt
     8221 acgcacaaga gttccgtagc tgttcaagtt tgtgtttcaa ctgttctcgt cgtttccgca
     8281 acaagtcctc ttcagaaatg agcttttgct cctctgcttg gacggacagg atgtatgctg
     8341 tggctttttt aaggataact accttggggg ccttttcatt gttttccaac tccgggatct
     8401 ggtcacgcag ggcaaaaaag ctccgtttta gctcgttcct cctctggcgc tccaagacgt
     8461 tgtgtgttcg cctcttgaca ttctcctcgg tgtccgagga cctggggctg gtgcattttc
     8521 ggttgttgct gatctgtctc aggactctga cactgtccaa cttgaccctc ttggcagcag
     8581 gatagtcctt ccgagtggag ggaggcgctg cgtagttgtg ctgatgtgtg gagacgtggc
     8641 acctcttgag gaccagtggg ctgtgaggag gtttgctgtg gcctccagca gaaggtgatc
     8701 cagactctga ccttttgcca ggagcctgcc tcttttccac agaaacaaca tcgatttctt
     8761 cctcatcttc ttgttcctcc tcagagtcgc tgctggtggt gggcggtgtc tcctcatgga
     8821 gcaccagggg ctcggggctg ccctgcgggg aggactccgt cgaggagagc agagaatccg
     8881 aggacggaga gaaggcgctg gagtcttgcg aggcgcagga cttgggcgag ctgctgtcgt
     8941 tgagagggta ggggaagacc accgaggggt cgatgcactc tgaggcggcg gcgctcagat
     9001 cctgcaggta caagctggag gtggagcaga cgctgtggcc gcgggcgggg ttcgggctgc
     9061 cgctgtcttt gcgcgcagcc tggtaggagg ccagcttctc tgagacgagc ttggcggcgg
     9121 ccgagaagcc gctccacata cagtcctgga tgatgatgtt tttgatgaag gtctcgtcgt
     9181 ccgggtcgca gatgaaactc tggttcacca tgtctcctcc cagcagctcg gtcaccatct
     9241 ccagctggtc ggccgtggag aagctcccgc caccgccgtc gttgtctccc cgaagggaga
     9301 agggtgtgac cgcaacgtag gagggcgagc agagcccgga gcggcggcta ggggacaggg
     9361 gcggggtggg cagcagctcg aatttcttcc agatatcctc gctgggcgcc gggggctgca
     9421 gctcgctctg ctgctgctgc tggtagaagt tctcctcctc gtcgcagtag aaatacggct
     9481 gcaccgagtc gtagtcgagg tcatagttcc tgttggtgaa gctaacgttg aggggcatgg
     9541 atcctctagt agagtcggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag
     9601 gcgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta
     9661 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
     9721 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
     9781 tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg
     9841 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     9901 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     9961 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
    10021 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
    10081 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
    10141 atatatgggc tatgaactaa tgaccccgta attgattact attaataact aatgcatggc
    10201 ggtaatacgt ctagaacctc ggacctgcag cacgtgttga caattaatca tcggcatagt
    10261 atatcggcat agtataatac gactcactat aggagggcca ccatggccaa gttgaccagt
    10321 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
    10381 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
    10441 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
    10501 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
    10561 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
    10621 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgattc
    10681 cggactagag cgggcccggt actaaccaaa ctggatctct gctgtccctg taataaaccc
    10741 gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
    10801 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat
    10861 tgtggatgaa tactgccatt tgtctgcaga attggcgcac gcagtgccga tccgttcact
    10921 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
    10981 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
    11041 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
    11101 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
    11161 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
    11221 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
    11281 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
    11341 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
    11401 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
    11461 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
    11521 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
    11581 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
    11641 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
    11701 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
    11761 aagatcagcg gccggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta
    11821 tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg
    11881 ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta
    11941 gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga
    12001 atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc
    12061 ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc
    12121 tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca
    12181 gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga
    12241 gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt
    12301 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
    12361 acacactact tgaagcactc aaggcaagct ttattgaggc tt
//
