LOCUS       sequence-456963-        9180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  guide RNA for Rab11 tagging at the N-terminus in pX459.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Stockhammer A, Adarska P, Natalia V, Heuhsen A, Klemt A, Bregu G, 
            Harel S, Rodilla-Ramirez C, Spalt C, Ozsoy E, Leupold P, Grindel A, 
            Fox E, Mejedo JO, Zehtabian A, Ewers H, Puchkov D, Haucke V, 
            Bottanelli F
  TITLE     ARF1 compartments direct cargo flow via maturation into recycling 
            endosomes.
  JOURNAL   Nat Cell Biol. 2024 Oct 4. doi: 10.1038/s41556-024-01518-4.
  PUBMED    39367144
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2024 Oct 4. doi: 10.1038/s41556-024-01518-4."
COMMENT     Sequence Label: Rab11 single guide RNA
FEATURES             Location/Qualifiers
     source          1..9180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..510
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(621..638)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    627..834
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   843..983
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   843..972
                     /label=AAV2 ITR
     rep_origin      1058..1513
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1145..1164)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1355..1376
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1530..1549)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1649..1671
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1709..1727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1795..1899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1900..1968,1969..2760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2118..2137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2931..3519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3420..3439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3581..3829
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3581..3601
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3752..3771
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3851..3926
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        4023..4308
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          4591..4818
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      4830..4839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4839..4904
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4911..4931
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4956..9056
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4956..9056
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9057..9104
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9120..9173
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(9174..9180,1..593)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(join(9174..9180,1..13))
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
       61 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
      121 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
      181 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
      241 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
      301 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
      361 ggttcctggc caccgtcgga gtctcgcccg accaccaggg caagggtctg ggcagcgccg
      421 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
      481 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
      541 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagaattct
      601 aactagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
      661 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
      721 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
      781 ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg gggagcggcc
      841 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
      901 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
      961 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     1021 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     1081 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     1141 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1201 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1261 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1321 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1381 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     1441 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     1501 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1561 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     1621 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     1681 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1741 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1801 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1861 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1921 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1981 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2041 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2101 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2161 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2221 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2281 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2341 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2401 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2461 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2521 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2581 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg
     2641 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2701 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2761 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2881 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3541 ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat ttcccatgat
     3601 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
     3661 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     3721 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     3781 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg cgtcgcgggt
     3841 gcccattgcg gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     3901 ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga gctagaaata gcaagttaaa
     3961 ataaggctag tccgttttta gcgcgtgcgc caattctgca gacaaatggc tctagaggta
     4021 cccgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     4081 ttgacgtcaa tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     4141 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     4201 gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc agtacatgac cttatgggac
     4261 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     4321 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     4381 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg ggggggcccg
     4441 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggc
     4501 aaataagggg ggggggcccc cgaatttcct ttttttgggc gggggggggg gggggggggc
     4561 cctaaaaaac gacgcagcgc gcggcggggg ggagtcgctg cgcgctgcct tcgccccgtg
     4621 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     4681 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagctgag caagaggtaa
     4741 gggtttaagg gatggttggt tggtggggta ttaatgttta attacctgga gcacctgcct
     4801 gaaatcactt tttttcaggt tggaccggtg ccaccatgga ctataaggac cacgacggag
     4861 actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga
     4921 agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg
     4981 acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
     5041 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
     5101 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
     5161 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
     5221 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
     5281 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
     5341 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
     5401 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
     5461 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
     5521 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
     5581 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
     5641 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc
     5701 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca
     5761 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt
     5821 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca
     5881 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc
     5941 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga
     6001 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg
     6061 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc
     6121 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca
     6181 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt
     6241 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct
     6301 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg
     6361 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga
     6421 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca
     6481 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg
     6541 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg
     6601 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca
     6661 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct
     6721 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg
     6781 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag
     6841 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc
     6901 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca
     6961 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca
     7021 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga
     7081 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca
     7141 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag
     7201 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca
     7261 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag
     7321 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga
     7381 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca
     7441 tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg
     7501 actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg
     7561 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca
     7621 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg
     7681 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc
     7741 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga
     7801 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt
     7861 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga
     7921 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt
     7981 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
     8041 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
     8101 ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg
     8161 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
     8221 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta
     8281 tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga
     8341 agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg
     8401 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg
     8461 aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag
     8521 tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc
     8581 ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct
     8641 ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg
     8701 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca
     8761 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag
     8821 tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca
     8881 tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca
     8941 ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc
     9001 agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaaaa
     9061 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggaattc ggcagtggag
     9121 agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccaatgaccg
//
