LOCUS       sequence-456974-        8347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression of PfAgo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8347)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pETM-30-GST-PfAgo_F
FEATURES             Location/Qualifiers
     source          1..8347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             130..780
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPP"
     CDS             817..837
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             847..3159
                     /codon_start=1
                     /product="Argonaute protein from the archaeon Pyrococcus 
                     furiosus"
                     /label=PfAgo
                     /note="DNA-guided nuclease (Swarts et al., 2015)"
                     /translation="MKAKVVINLVKINKKIIPDKIYVYRLFNDPEEELQKEGYSIYRLA
                     YENVGIVIDPENLIIATTKELEYEGEFIPEGEISFSELRNDYQSKLVLRLLKENGIGEY
                     ELSKLLRKFRKPKTFGDYKVIPSVEMSVIKHDEDFYLVIHIIHQIQSMKTLWELVNKDP
                     KELEEFLMTHKENLMLKDIASPLKTVYKPCFEEYTKKPKLDHNQEIVKYWYNYHIERYW
                     NTPEAKLEFYRKFGQVDLKQPAILAKFASKIKKNKNYKIYLLPQLVVPTYNAEQLESDV
                     AKEILEYTKLMPEERKELLENVLAEVDSDIIDKSLSEIEVEKIAQELENKIRVRDDKGN
                     SVPISQLNVQKSQLLLWTNYSRKYPVILPYEVPEKFRKIREIPMFIILDSGLLADIQNF
                     ATNEFRELVKSMYYSLAKKYNSLAKKARSTNEIGLPFLDFRGKEKVITEDLNSDKGIIE
                     VVEQVSSFMKGKELGLAFIAARNKLSSEKFEEIKRRLFNLNVISQVVNEDTLKNKRDKY
                     DRNRLDLFVRHNLLFQVLSKLGVKYYVLDYRFNYDYIIGIDVAPMKRSEGYIGGSAVMF
                     DSQGYIRKIVPIKIGEQRGESVDMNEFFKEMVDKFKEFNIKLDNKKILLLRDGRITNNE
                     EEGLKYISEMFDIEVVTMDVIKNHPVRAFANMKMYFNLGGAIYLIPHKLKQAKGTPIPI
                     KLAKKRIIKNGKVEKQSITRQDVLDIFILTRLNYGSISADMRLPAPVHYAHKFANAIRN
                     EWKIKEEFLAEGFLYFV"
     CDS             3212..3229
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3282..3300)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3296..3343
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3380..3835
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3467..3486)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3677..3698
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3927..4742)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3927..4742)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4650..4669
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4734..4753
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4864..5452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5353..5372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5606..5623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5638..5777
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5703..5722)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5863..5885
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5879..6070)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6879..7961)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7923..7942
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7962..8039)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     8245..8264
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg aaacatcacc atcaccatca caacactagt
      121 agcaattcca tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact
      181 cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa
      241 ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat
      301 tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac
      361 aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga
      421 gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact
      481 ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta
      541 tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac
      601 gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt
      661 tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa atccagcaag
      721 tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca
      781 actagtggat ctggtggtgg tggcggatgg atgagcgaga atctttattt tcagggcgcc
      841 atggcaatga aagcgaaagt tgttattaat ctggtaaaga taaataaaaa aataattcca
      901 gataaaatat atgtatatag gctctttaat gacccagagg aagaacttca aaaagaggga
      961 tattctattt atcgccttgc atatgaaaac gttggtattg ttattgatcc agagaatctg
     1021 attattgcca ccacaaaaga gcttgaatat gagggagaat ttattcccga gggtgaaata
     1081 tcattctcgg aattgcgtaa tgactatcag agtaaacttg tcttaagatt gttgaaggag
     1141 aatggaatag gtgaatatga attatcgaaa cttcttagga agtttagaaa accaaaaact
     1201 tttggggatt ataaggtcat tccaagcgtt gagatgagtg tgataaagca tgatgaagat
     1261 ttttatttag taattcatat aattcatcaa atacaatcaa tgaaaacact gtgggagctt
     1321 gtaaacaaag accctaaaga gcttgaagag tttttaatga ctcacaagga aaatttaatg
     1381 ctgaaggaca ttgcgtcacc acttaaaact gtttataagc cttgctttga agagtatact
     1441 aaaaaaccaa aacttgatca taatcaagaa atagtaaagt actggtataa ttatcatatt
     1501 gaaagatatt ggaatactcc agaagctaaa ttagagtttt atagaaaatt tggccaagtt
     1561 gatttaaaac aacctgcaat tctagcgaaa tttgcatcta aaataaagaa aaacaaaaat
     1621 tacaagatat atctcttgcc ccaattagtt gtcccaactt ataacgccga acaattagaa
     1681 agtgacgtgg cgaaagaaat tttagaatat acaaaactga tgccagaaga acgtaaagag
     1741 cttttagaaa acgtccttgc agaagttgat agtgacatta tagacaaatc attaagtgaa
     1801 attgaagtag aaaaaattgc acaagaattg gaaaacaaaa taagggttag agatgacaaa
     1861 gggaacagtg taccaatttc tcaattaaat gttcaaaaat ctcaattact actttggacg
     1921 aattattcac gaaaatatcc agttatattg ccttatgaag ttccagaaaa gttcagaaaa
     1981 atacgggaga taccaatgtt tataattcta gattcaggac ttcttgccga cattcaaaat
     2041 tttgcaacta atgaattcag ggagttagta aagagcatgt actatagtct tgccaaaaaa
     2101 tacaatagtc ttgccaaaaa agcaaggtca acaaatgaaa taggattacc ctttttagac
     2161 tttcgtggga aagaaaaggt tataactgaa gatttaaact ctgacaaagg tattatagaa
     2221 gttgtggagc aggtatctag tttcatgaaa ggaaaagaac taggcctagc ctttatagct
     2281 gcaagaaata aactttcatc tgagaagttt gaggaaatta aaaggagact ctttaattta
     2341 aatgtaatat ctcaagtggt taacgaagat actttaaaaa ataaaagaga caaatacgat
     2401 agaaatcgac ttgatctatt tgtcagacac aacttacttt ttcaagtatt atctaaactt
     2461 ggagtaaagt attatgtgct agattacagg ttcaattatg actacatcat tggaattgat
     2521 gttgctccca tgaagcgttc tgagggatac ataggtggta gtgccgtaat gtttgactct
     2581 caaggttaca tacgaaaaat tgtcccaatt aaaattggtg aacaaagagg agaatcagtt
     2641 gacatgaatg agtttttcaa agaaatggtt gataaattta aagagttcaa tattaagcta
     2701 gataataaga aaatactact tctaagagat ggaagaatta ctaataacga agaagaaggg
     2761 ctcaagtata tttccgagat gtttgatatt gaggtggtca cgatggacgt gataaaaaat
     2821 caccctgtga gagcctttgc taatatgaaa atgtacttta acctaggtgg tgcaatatac
     2881 ctaatccccc ataagctcaa acaagctaaa ggaacaccaa taccaataaa gttagccaaa
     2941 aagaggataa ttaagaatgg aaaagtcgag aagcagagta tcactagaca ggacgtctta
     3001 gatatcttca ttttaactcg tcttaactat gggagtattt ctgctgatat gcggttgcca
     3061 gcacccgttc actatgctca caagtttgca aatgcaatca gaaatgaatg gaaaataaaa
     3121 gaagagttcc ttgctgaggg atttttgtat tttgtttgag gtaccggatc cgaattcgag
     3181 ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
     3241 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     3301 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     3361 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3421 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3481 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3541 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3601 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3661 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3721 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3781 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     3841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3901 atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc
     3961 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     4021 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     4081 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     4141 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt
     4201 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     4261 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
     4321 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     4381 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     4441 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     4501 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     4561 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     4621 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     4681 gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc
     4741 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgaccaaaat
     4801 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4861 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4921 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4981 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5041 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5101 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5161 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5221 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5281 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5341 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5401 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5461 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5521 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5581 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     5641 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg gtgcactctc
     5701 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     5761 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     5821 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     5881 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     5941 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     6001 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     6061 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     6121 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     6181 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     6241 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     6301 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     6361 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     6421 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     6481 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtgggg
     6541 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
     6601 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
     6661 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
     6721 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
     6781 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
     6841 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6901 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6961 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
     7021 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
     7081 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
     7141 tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc
     7201 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
     7261 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
     7321 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
     7381 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
     7441 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
     7501 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
     7561 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
     7621 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
     7681 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
     7741 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
     7801 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
     7861 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
     7921 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
     7981 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
     8041 gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     8101 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     8161 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     8221 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     8281 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
     8341 gcgaaat
//
