LOCUS       sequence-456980-        9277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  guide RNA for AP1muA tagging at the C-terminus in pX459.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Stockhammer A, Spalt C, Klemt A, Benz LS, Harel S, Natalia V, Wiench
            L, Freund C, Kuropka B, Bottanelli F
  TITLE     When less is more - a fast TurboID knock-in approach for 
            high-sensitivity endogenous interactome mapping.
  JOURNAL   J Cell Sci. 2024 Aug 15;137(16):jcs261952. doi: 10.1242/jcs.261952. 
            Epub 2024 Aug 28.
  PUBMED    39056144
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2024-08-15"; volume: "137(16):jcs261952. doi"; pages: 
            " 10.1242/jcs.261952. Epub 2024 Aug 28"
COMMENT     Sequence Label: AP1muA single guide RNA
FEATURES             Location/Qualifiers
     source          1..9277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     356..376
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(487..504)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    493..700
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   709..849
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   709..838
                     /label=AAV2 ITR
     rep_origin      924..1379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1011..1030)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1221..1242
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1396..1415)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1515..1537
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1575..1593)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1661..1765
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1766..1834,1835..2626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1984..2003)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2797..3385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3286..3305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3447..3695
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3447..3467
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3618..3637
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3717..3792
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        3889..4174
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          4554..4781
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      4793..4802
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4802..4867
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4874..4894
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4919..9019
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4919..9019
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9020..9067
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9083..9136
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(9137..9277,1..459)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9137..9156)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
       61 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
      121 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
      181 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
      241 gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
      301 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
      361 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
      421 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag aattctaact agagctcgct
      481 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
      541 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
      601 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
      661 agggggagga ttgggaagag aatagcaggc atgctgggga gcggccgcag gaacccctag
      721 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
      781 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
      841 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
      901 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      961 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     1021 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     1081 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     1141 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     1201 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
     1261 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
     1321 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     1381 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     1441 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     1501 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     1561 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     1621 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1681 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1741 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1801 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1861 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1921 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1981 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2041 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2101 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2161 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2221 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2281 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2341 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2401 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2461 atctggagcc ggtgagcgtg gaagccgcgg tatcattgca gcactggggc cagatggtaa
     2521 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2581 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2641 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2701 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2761 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2821 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2881 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2941 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3001 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3061 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3121 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3181 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3241 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3301 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3361 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3421 cttttgctgg ccttttgctc acatgtgagg gcctatttcc catgattcct tcatatttgc
     3481 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     3541 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     3601 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     3661 tggctttata tatcttgtgg aaaggacgaa acaccgcagc caacaccccg gcctcggttt
     3721 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     3781 ccgagtcggt gcttttttgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     3841 tttttagcgc gtgcgccaat tctgcagaca aatggctcta gaggtacccg ttacataact
     3901 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaatagt
     3961 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     4021 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     4081 taaatggccc gcctggcatt gtgcccagta catgacctta tgggactttc ctacttggca
     4141 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     4201 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     4261 attttgtgca gcgatggggg cggggggggg gggggggggg cgccgggggg gggggggggg
     4321 gggggggggg gggggggggg gggggggggg gggggggggg ggggcgccca ggggggggcg
     4381 cccccgcagg tttttttttt ggggcggggg ggggggggcg gggggggggg gggggggggg
     4441 gggggcggag aggtgcggcg gcggccaatc agggcggcgc gctccgaaag tttcctttta
     4501 tggcgaggcg ggggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     4561 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     4621 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     4681 gtaattagct gagcaagagg taagggttta agggatggtt ggttggtggg gtattaatgt
     4741 ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat
     4801 ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga
     4861 cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccga
     4921 caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac
     4981 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
     5041 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
     5101 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
     5161 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
     5221 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
     5281 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
     5341 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
     5401 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
     5461 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
     5521 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
     5581 ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat
     5641 tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc
     5701 caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat
     5761 cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct
     5821 gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat
     5881 caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca
     5941 gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta
     6001 cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa
     6061 gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca
     6121 gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat
     6181 tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa
     6241 gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt
     6301 cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt
     6361 ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct
     6421 gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa
     6481 cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg
     6541 cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa
     6601 gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg
     6661 cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa
     6721 ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac
     6781 cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct
     6841 gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct
     6901 gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt
     6961 cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct
     7021 gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga
     7081 gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa
     7141 ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga
     7201 aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa
     7261 gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga
     7321 aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat
     7381 gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt
     7441 gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa
     7501 gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta
     7561 ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa
     7621 ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt
     7681 ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa
     7741 gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct
     7801 ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca
     7861 ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc
     7921 taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat
     7981 cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat
     8041 catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct
     8101 gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac
     8161 cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac
     8221 aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag
     8281 aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc
     8341 tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga
     8401 gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct
     8461 ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc
     8521 cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa
     8581 gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta
     8641 tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca
     8701 caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct
     8761 ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat
     8821 cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc
     8881 cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt
     8941 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
     9001 gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa
     9061 gaaaaaggaa ttcggcagtg gagagggcag aggaagtctg ctaacatgcg gtgacgtcga
     9121 ggagaatcct ggcccaatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     9181 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     9241 caccgtcgat ccggaccgcc acatcgagcg ggtcacc
//
