LOCUS       sequence-457010-        9018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for generation of donor vectors for CRISPR/Cas9 mediated 
            Saturation Genome Editing (SGE) of TP53 Exon 6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Funk JS, Klimovich M, Drangenstein D, Pielhoop O, Hunold P, Borowek 
            A, Noeparast M, Pavlakis E, Neumann M, Balourdas DI, Kochhan K, 
            Merle N, Bullwinkel I, Wanzel M, Elmshauser S, Teply-Szymanski J, 
            Nist A, Procida T, Bartkuhn M, Humpert K, Mernberger M, Savai R, 
            Soussi T, Joerger AC, Stiewe T
  TITLE     Deep CRISPR mutagenesis characterizes the functional diversity of 
            TP53 mutations.
  JOURNAL   Nat Genet. 2025 Jan;57(1):140-153. doi: 10.1038/s41588-024-02039-4. 
            Epub 2025 Jan 7.
  PUBMED    39774325
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-024-02039-4"; journalName: "Nat Genet"; date: 
            "2025-01"; volume: "57"; issue: "1"; pages: "140-153"
COMMENT     Sequence Label: HR700_HA2TP53_Ex6
FEATURES             Location/Qualifiers
     source          1..9018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(805..838)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1115..1326
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1339..1607
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      1635..1644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1665..2327
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             2403..2456
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2457..3056
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2457..2476)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2953..2973
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3094..3111)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3100..3307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3551..3584)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     5205..5224
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      5280..5289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5286..6416
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    6417..6538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6454..6473)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6508..6527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6572..6588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6572..6588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6585..6607)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6596..6612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6620..6626,6627..6644,6645..6650))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6665..6686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6803..6820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6974..7562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7054..7073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7883..8698)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7883..8698)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8606..8625
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8690..8709
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    8817..8835
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     8991..9009
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaaac tagtggtacc tgtacaagag
      421 gctgctcccc ccgtggcccc tgcaccagca gctcctacac cggcggcccc tgcaccagcc
      481 ccctcctggc ccctgtcatc ttctgtccct tcccagaaaa cctaccaggg cagctacggt
      541 ttccgtctgg gcttcttgca ttctgggaca gccaagtctg tgacttgcac ggtcagttgc
      601 cctgaggggc tggcttccat gagacttcaa tgcctggccg tatccccctg catttctttt
      661 gtttggaact ttgggattcc tcttcaccct ttggcttcct gtcagtgttt ttttatagtt
      721 tacccactta atgtgtgatc tctgactcct gtcccaaagt tgaatattcc ccccttgaat
      781 ttgggctttt atccatcggt ctctataact tcgtatagca tacattatac gaagttatgc
      841 tagcgaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
      901 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
      961 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     1021 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaagcta
     1081 gcaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     1141 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     1201 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     1261 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     1321 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     1381 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     1441 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gtccgggcct ttgtccggcg
     1501 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     1561 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     1621 gcgcctacga attcgccacc atggagagcg acgagagcgg cctgcccgcc atggagatcg
     1681 agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg
     1741 gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct
     1801 tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc
     1861 ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc
     1921 gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg
     1981 ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga
     2041 tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg
     2101 ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca
     2161 gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga
     2221 acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg
     2281 gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga tcccgcgctc
     2341 agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgcagta
     2401 ctgagggcag aggaagtctc ctaacatgcg gtgacgtgga ggagaatccc ggccctatga
     2461 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     2521 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     2581 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     2641 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     2701 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     2761 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     2821 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     2881 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     2941 catccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     3001 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggat
     3061 cctgatcgag tctagagggc ccccgctgat cagcctcgac tgtgccttct agttgccagc
     3121 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3181 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3241 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg
     3301 ctggggaaga tctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     3361 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     3421 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     3481 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     3541 ccctagatct ataacttcgt atagcataca ttatacgaag ttatgagaat tcacacgcgt
     3601 ccatcacacc ctcagcatct ctcctgggga tgcagaactt ttctttttct tcatccacgt
     3661 gtattccttg gcttttgaaa ataagctcct gaccaggctt ggtggctcac acctgcaatc
     3721 ccagcactct caaagaggcc aaggcaggca gatcacctga gcccaggagt tcaagaccag
     3781 cctgggtaac atgatgaaac ctcgtctcta caaaaaaata caaaaaatta gccaggcatg
     3841 gtggtgcaca cctatagtcc cagccactta ggaggctgag gtgggaagat cacttgaggc
     3901 caggagatgg aggctgcagt gagctgtgat cacaccactg tgctccagcc tgagtgacag
     3961 agcaagaccc tatctcaaaa aaaaaaaaaa aaaagaaaag ctcctgaggt gtagacgcca
     4021 actctctcta gctcgctagt gggttgcagg aggtgcttac gcatgtttgt ttctttgctg
     4081 ccgaattcca gttgctttat ctgttcactt gtgccctgac tttcaactct gtctccttcc
     4141 tcttcctaca gtactcccct gccctcaaca agatgttttg ccaactggcc aagacctgcc
     4201 ctgtgcagct gtgggttgat tccacacccc cgcccggcac ccgcgtccgc gccatggcca
     4261 tctacaagca gtcacagcac atgacggagg ttgtgaggcg ctgcccccac catgagcgct
     4321 gctcagatag cgatggtgag cagctggggc tggagagacg acagggctgg ttgcccaggg
     4381 tccggaggcc tctgattccg tcttctcact gatgcaactg ggaagacggt ctctgggagg
     4441 aggggttaag ggtggttgtc agtggccctc caggtgagca gtaggggggc tttctcctgc
     4501 tgcttatttg acctccctat aaccccatga gatgtgcaaa gtaaatgggt ttaactattg
     4561 cacagttgaa aaaactgaag cttacagagg ctaagggcct cccctgcttg gctgggcgca
     4621 gtggctcatg cctgtaatcc cagcactttg ggaggccaag gcaggcggat cacgaggttg
     4681 ggagatcgag accatcctgg ctaacggtga aaccccgtct ctactgaaaa atacaaaaaa
     4741 aaattagccg ggcgtggtgc tgggcacctg tagtcccagc tactcgggag gctgaggaag
     4801 gagaatggcg tgaacctggg cggtggagct tgcagtgagc tgagatcacg ccactgcact
     4861 ccagcctggg cgacagagcg agattccatc tcgtcgacac ccctctggcc tcgcacacat
     4921 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     4981 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     5041 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     5101 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     5161 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     5221 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     5281 ccaccatggc ttcgtacccc ggccatcaac acgcctctgc gttcgaccag gctgcgcgtt
     5341 ctcgcggcca tagcaaccga cgtacggcgt tgcgccctcg ccggcagcaa gaagccacgg
     5401 aagtccgccc ggagcagaaa atgcccacgc tactgcgggt ttatatagac ggtccccacg
     5461 ggatggggaa aaccaccacc acgcaactgc tggtggccct gggttcgcgc gacgatatcg
     5521 tctacgtacc cgagccgatg acttactggc gggtgctggg ggcttccgag acaatcgcga
     5581 acatctacac cacacaacac cgcctcgacc agggtgagat atcggccggg gacgcggcgg
     5641 tggtaatgac aagcgcccag ataacaatgg gcatgcctta tgccgtgacc gacgccgttc
     5701 tggctcctca tatcgggggg gaggctggga gctcacatgc cccgcccccg gccctcaccc
     5761 tcatcttcga ccgccatccc atcgccgccc tcctgtgcta cccggccgcg cggtacctta
     5821 tgggcagcat gaccccccag gccgtgctgg cgttcgtggc cctcatcccg ccgaccttgc
     5881 ccggcaccaa catcgtgctt ggggcccttc cggaggacag acacatcgac cgcctggcca
     5941 aacgccagcg ccccggcgag cggctggacc tggctatgct ggctgcgatt cgccgcgttt
     6001 acgggctact tgccaatacg gtgcggtatc tgcagtgcgg cgggtcgtgg cgggaggact
     6061 ggggacagct ttcggggacg gccgtgccgc cccagggtgc cgagccccag agcaacgcgg
     6121 gcccacgacc ccatatcggg gacacgttat ttaccctgtt tcgggccccc gagttgctgg
     6181 cccccaacgg cgacctgtat aacgtgtttg cctgggcctt ggacgtcttg gccaaacgcc
     6241 tccgttccat gcacgtcttt atcctggatt acgaccaatc gcccgccggc tgccgggacg
     6301 ccctgctgca acttacctcc gggatggtcc agacccacgt caccaccccc ggctccatac
     6361 cgacgatatg cgacctggcg cgcacgtttg cccgggagat gggggaggct aactgaaact
     6421 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6481 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6541 atgtctggat atcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6601 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6721 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6781 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7381 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgatccccg
     7681 ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt
     7741 gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa
     7801 gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt
     7861 acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt
     7921 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     7981 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     8041 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     8101 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     8161 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     8221 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     8281 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     8341 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttt ccggggatcg
     8401 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     8461 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     8521 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     8581 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     8641 ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa
     8701 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     8761 tatcttgtgc aatgtaacat cagagatttt gagacacaat tcatcgatga tggttgagat
     8821 gtgtataaga gacagattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8881 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8941 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     9001 tcacgaggcc ctttcgtc
//
