LOCUS       sequence-457012-        8947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for generation of donor vectors for CRISPR/Cas9 mediated 
            Saturation Genome Editing (SGE) of TP53 Exon 5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Funk JS, Klimovich M, Drangenstein D, Pielhoop O, Hunold P, Borowek 
            A, Noeparast M, Pavlakis E, Neumann M, Balourdas DI, Kochhan K, 
            Merle N, Bullwinkel I, Wanzel M, Elmshauser S, Teply-Szymanski J, 
            Nist A, Procida T, Bartkuhn M, Humpert K, Mernberger M, Savai R, 
            Soussi T, Joerger AC, Stiewe T
  TITLE     Deep CRISPR mutagenesis characterizes the functional diversity of 
            TP53 mutations.
  JOURNAL   Nat Genet. 2025 Jan;57(1):140-153. doi: 10.1038/s41588-024-02039-4. 
            Epub 2025 Jan 7.
  PUBMED    39774325
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-024-02039-4"; journalName: "Nat Genet"; date: 
            "2025-01"; volume: "57"; issue: "1"; pages: "140-153"
COMMENT     Sequence Label: HR700_HA2TP53_Ex5
FEATURES             Location/Qualifiers
     source          1..8947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(37..625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(117..136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(946..1761)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(946..1761)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1669..1688
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1753..1772
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    1880..1898
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     2054..2072
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2110..2132)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2232..2251
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2445..2467
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2459..2476
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2460..2476
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2886..2919)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3196..3407
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3420..3688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3716..3725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3746..4408
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             4484..4537
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4538..5137
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4538..4557)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5034..5054
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5175..5192)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5181..5388
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(5632..5665)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     7215..7234
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      7290..7299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7296..8426
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    8427..8548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8464..8483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8518..8537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8582..8598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8582..8598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8595..8617)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8606..8622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8630..8636,8637..8654,8655..8660))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8675..8696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8813..8830)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
       61 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      121 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      181 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      241 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      301 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      361 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      421 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      481 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      541 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      601 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      661 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
      721 cacctagatc cttttgatcc ccgccacggt tgatgagagc tttgttgtag gtggaccagt
      781 tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga
      841 tctgatcctt caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt
      901 cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc
      961 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     1021 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     1081 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     1141 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     1201 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     1261 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     1321 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     1381 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     1441 gaatgctgtt tttccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     1501 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     1561 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     1621 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     1681 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt
     1741 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     1801 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     1861 aattcatcga tgatggttga gatgtgtata agagacagat tattgaagca tttatcaggg
     1921 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     1981 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     2041 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     2101 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     2161 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     2221 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     2281 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     2341 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     2401 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     2461 taaaacgacg gccagtgaaa ctagtggtac ctgtacaaga ggctgctccc cccgtggccc
     2521 ctgcaccagc agctcctaca ccggcggccc ctgcaccagc cccctcctgg cccctgtcat
     2581 cttctgtccc ttcccagaaa acctaccagg gcagctacgg tttccgtctg ggcttcttgc
     2641 attctgggac agccaagtct gtgacttgca cggtcagttg ccctgagggg ctggcttcca
     2701 tgagacttca atgcctggcc gtatccccct gcatttcttt tgtttggaac tttgggattc
     2761 ctcttcaccc tttggcttcc tgtcagtgtt tttttatagt ttacccactt aatgtgtgat
     2821 ctctgactcc tgtcccaaag ttgaatattc cccccttgaa tttgggcttt tatccatcgg
     2881 tctctataac ttcgtatagc atacattata cgaagttatg ctagcgaggg acagcccccc
     2941 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
     3001 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
     3061 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
     3121 tttgagcctg cagacacctg gggggatacg gggaaaagct agcaaggatc tgcgatcgct
     3181 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     3241 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     3301 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     3361 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag
     3421 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
     3481 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
     3541 taagtttaaa gctcaggtcg agtccgggcc tttgtccggc gctcccttgg agcctaccta
     3601 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
     3661 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg aattcgccac
     3721 catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac
     3781 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg
     3841 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag
     3901 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc
     3961 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga
     4021 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga
     4081 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat
     4141 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag
     4201 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca
     4261 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc
     4321 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca
     4381 cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt
     4441 ggacggcacc gccggacccg gctccaccgg atctcgcagt actgagggca gaggaagtct
     4501 cctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt
     4561 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4621 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4681 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4741 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4801 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4861 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4921 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4981 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct
     5041 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5101 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagga tcctgatcga gtctagaggg
     5161 cccccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     5221 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     5281 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     5341 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggaag atctttttcc
     5401 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     5461 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     5521 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     5581 gggacgtaat tacatccctg ggggctttgg gggggggctg tccctagatc tataacttcg
     5641 tatagcatac attatacgaa gttatgagaa ttcacacgcg tccatcacac cctcagcatc
     5701 tctcctgggg atgcagaact tttctttttc ttcatccacg tgtattcctt ggcttttgaa
     5761 aataagctcc tgaccaggct tggtggctca cacctgcaat cccagcactc tcaaagaggc
     5821 caaggcaggc agatcacctg agcccaggag ttcaagacca gcctgggtaa catgatgaaa
     5881 cctcgtctct acaaaaaaat acaaaaaatt agccaggcat ggtggtgcac acctatagtc
     5941 ccagccactt aggaggctga ggtgggaaga tcacttgagg ccaggagatg gaggctgcag
     6001 tgagctgtga tcacaccact gtgctccagc ctgagtgaca gagcaagacc ctatctcaaa
     6061 aaaaaaaaaa aaaaagaaaa gctcctgagg tgtagacgcc aactctctct agctcgctag
     6121 tgggttgcag gaggtgctta cgcatgtttg tttctttgct gccgaattcc agttgcttta
     6181 tctgttcact tgtgccctga ctttcaactc tgtctgtctt cccttcctct ggggctggaa
     6241 gacgagagac gacagggctg gttgcccagg gtccggaggc ctctgattcc tcactgattg
     6301 ctcttaggtc tggcccctcc tcagcatctt atccgagtgg aaggaaattt gcgtgtggag
     6361 tatttggatg acagaaacac ttttcgacat agtgtggtgg tgccctatga gccgcctgag
     6421 gtctggtttg caactggggt ctctgggagg aggggttaag ggtggttgtc agtggccctc
     6481 caggtgagca gtaggggggc tttctcctgc tgcttatttg acctccctat aaccccatga
     6541 gatgtgcaaa gtaaatgggt ttaactattg cacagttgaa aaaactgaag cttacagagg
     6601 ctaagggcct cccctgcttg gctgggcgca gtggctcatg cctgtaatcc cagcactttg
     6661 ggaggccaag gcaggcggat cacgaggttg ggagatcgag accatcctgg ctaacggtga
     6721 aaccccgtct ctactgaaaa atacaaaaaa aaattagccg ggcgtggtgc tgggcacctg
     6781 tagtcccagc tactcgggag gctgaggaag gagaatggcg tgaacctggg cggtggagct
     6841 tgcagtgagc tgagatcacg ccactgcact ccagcctggg cgacagagcg agattccatc
     6901 tcgtcgacac ccctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     6961 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     7021 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     7081 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     7141 caatagcagc tttgctcctt cgctttctgg gctcaggggc ggggcgggcg cccgaaggtc
     7201 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     7261 ttcctcatct ccgggccttt cgacctgcag ccaccatggc ttcgtacccc ggccatcaac
     7321 acgcctctgc gttcgaccag gctgcgcgtt ctcgcggcca tagcaaccga cgtacggcgt
     7381 tgcgccctcg ccggcagcaa gaagccacgg aagtccgccc ggagcagaaa atgcccacgc
     7441 tactgcgggt ttatatagac ggtccccacg ggatggggaa aaccaccacc acgcaactgc
     7501 tggtggccct gggttcgcgc gacgatatcg tctacgtacc cgagccgatg acttactggc
     7561 gggtgctggg ggcttccgag acaatcgcga acatctacac cacacaacac cgcctcgacc
     7621 agggtgagat atcggccggg gacgcggcgg tggtaatgac aagcgcccag ataacaatgg
     7681 gcatgcctta tgccgtgacc gacgccgttc tggctcctca tatcgggggg gaggctggga
     7741 gctcacatgc cccgcccccg gccctcaccc tcatcttcga ccgccatccc atcgccgccc
     7801 tcctgtgcta cccggccgcg cggtacctta tgggcagcat gaccccccag gccgtgctgg
     7861 cgttcgtggc cctcatcccg ccgaccttgc ccggcaccaa catcgtgctt ggggcccttc
     7921 cggaggacag acacatcgac cgcctggcca aacgccagcg ccccggcgag cggctggacc
     7981 tggctatgct ggctgcgatt cgccgcgttt acgggctact tgccaatacg gtgcggtatc
     8041 tgcagtgcgg cgggtcgtgg cgggaggact ggggacagct ttcggggacg gccgtgccgc
     8101 cccagggtgc cgagccccag agcaacgcgg gcccacgacc ccatatcggg gacacgttat
     8161 ttaccctgtt tcgggccccc gagttgctgg cccccaacgg cgacctgtat aacgtgtttg
     8221 cctgggcctt ggacgtcttg gccaaacgcc tccgttccat gcacgtcttt atcctggatt
     8281 acgaccaatc gcccgccggc tgccgggacg ccctgctgca acttacctcc gggatggtcc
     8341 agacccacgt caccaccccc ggctccatac cgacgatatg cgacctggcg cgcacgtttg
     8401 cccgggagat gggggaggct aactgaaact tgtttattgc agcttataat ggttacaaat
     8461 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8521 gtttgtccaa actcatcaat gtatcttatc atgtctggat atcaagcttg gcgtaatcat
     8581 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8641 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8701 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8761 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8821 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8881 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8941 agcaaaa
//
