LOCUS       sequence-457015-       10226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for generation of donor vectors for CRISPR/Cas9 mediated 
            Saturation Genome Editing (SGE) of TP53 Exon 8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Funk JS, Klimovich M, Drangenstein D, Pielhoop O, Hunold P, Borowek 
            A, Noeparast M, Pavlakis E, Neumann M, Balourdas DI, Kochhan K, 
            Merle N, Bullwinkel I, Wanzel M, Elmshauser S, Teply-Szymanski J, 
            Nist A, Procida T, Bartkuhn M, Humpert K, Mernberger M, Savai R, 
            Soussi T, Joerger AC, Stiewe T
  TITLE     Deep CRISPR mutagenesis characterizes the functional diversity of 
            TP53 mutations.
  JOURNAL   Nat Genet. 2025 Jan;57(1):140-153. doi: 10.1038/s41588-024-02039-4. 
            Epub 2025 Jan 7.
  PUBMED    39774325
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-024-02039-4"; journalName: "Nat Genet"; date: 
            "2025-01"; volume: "57"; issue: "1"; pages: "140-153"
COMMENT     Sequence Label: HR700_HA2TP53_Ex8
FEATURES             Location/Qualifiers
     source          1..10226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(805..838)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1115..1326
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1339..1607
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      1635..1644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1665..2327
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             2403..2456
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2457..3056
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2457..2476)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2953..2973
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3094..3111)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3100..3307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3551..3584)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     6413..6432
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6488..6497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6494..7624
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    7625..7746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7662..7681)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7716..7735
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7780..7796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7780..7796)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7793..7815)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7804..7820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7828..7834,7835..7852,7853..7858))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7873..7894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8011..8028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8182..8770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8262..8281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9091..9906)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9091..9906)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9814..9833
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9898..9917
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    10025..10043
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     10199..10217
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaaac tagtggtacc tgtacaagag
      421 gctgctcccc ccgtggcccc tgcaccagca gctcctacac cggcggcccc tgcaccagcc
      481 ccctcctggc ccctgtcatc ttctgtccct tcccagaaaa cctaccaggg cagctacggt
      541 ttccgtctgg gcttcttgca ttctgggaca gccaagtctg tgacttgcac ggtcagttgc
      601 cctgaggggc tggcttccat gagacttcaa tgcctggccg tatccccctg catttctttt
      661 gtttggaact ttgggattcc tcttcaccct ttggcttcct gtcagtgttt ttttatagtt
      721 tacccactta atgtgtgatc tctgactcct gtcccaaagt tgaatattcc ccccttgaat
      781 ttgggctttt atccatcggt ctctataact tcgtatagca tacattatac gaagttatgc
      841 tagcgaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
      901 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
      961 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     1021 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaagcta
     1081 gcaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     1141 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     1201 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     1261 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     1321 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     1381 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     1441 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gtccgggcct ttgtccggcg
     1501 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     1561 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     1621 gcgcctacga attcgccacc atggagagcg acgagagcgg cctgcccgcc atggagatcg
     1681 agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg
     1741 gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct
     1801 tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc
     1861 ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc
     1921 gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg
     1981 ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga
     2041 tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg
     2101 ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca
     2161 gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga
     2221 acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg
     2281 gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga tcccgcgctc
     2341 agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgcagta
     2401 ctgagggcag aggaagtctc ctaacatgcg gtgacgtgga ggagaatccc ggccctatga
     2461 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     2521 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     2581 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     2641 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     2701 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     2761 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     2821 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     2881 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     2941 catccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     3001 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggat
     3061 cctgatcgag tctagagggc ccccgctgat cagcctcgac tgtgccttct agttgccagc
     3121 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3181 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3241 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg
     3301 ctggggaaga tctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     3361 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     3421 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     3481 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     3541 ccctagatct ataacttcgt atagcataca ttatacgaag ttatgagaat tcacacgcgt
     3601 ccatcacacc ctcagcatct ctcctgggga tgcagaactt ttctttttct tcatccacgt
     3661 gtattccttg gcttttgaaa ataagctcct gaccaggctt ggtggctcac acctgcaatc
     3721 ccagcactct caaagaggcc aaggcaggca gatcacctga gcccaggagt tcaagaccag
     3781 cctgggtaac atgatgaaac ctcgtctcta caaaaaaata caaaaaatta gccaggcatg
     3841 gtggtgcaca cctatagtcc cagccactta ggaggctgag gtgggaagat cacttgaggc
     3901 caggagatgg aggctgcagt gagctgtgat cacaccactg tgctccagcc tgagtgacag
     3961 agcaagaccc tatctcaaaa aaaaaaaaaa aaaagaaaag ctcctgaggt gtagacgcca
     4021 actctctcta gctcgctagt gggttgcagg aggtgcttac gcatgtttgt ttctttgctg
     4081 ccgaattcca gttgctttat ctgttcactt gtgccctgac tttcaactct gtctccttcc
     4141 tcttcctaca gtactcccct gccctcaaca agatgttttg ccaactggcc aagacctgcc
     4201 ctgtgcagct gtgggttgat tccacacccc cgcccggcac ccgcgtccgc gccatggcca
     4261 tctacaagca gtcacagcac atgacggagg ttgtgaggcg ctgcccccac catgagcgct
     4321 gctcagatag cgatggtgag cagctggggc tggagagacg acagggctgg ttgcccaggg
     4381 tccccaggcc tctgattcct cactgattgc tcttaggtct ggcccctcct cagcatctta
     4441 tccgagtgga aggaaatttg cgtgtggagt atttggatga cagaaacact tttcgacata
     4501 gtgtggtggt gccctatgag ccgcctgagg tctggtttgc aactggggtc tctgggagga
     4561 ggggttaagg gtggttgtca gtggccctcc aggtgagcag taggggggct ttctcctgct
     4621 gcttatttga cctccctata accccatgag atgtgcaaag taaatgggtt taactattgc
     4681 acagttgaaa aaactgaagc ttacagaggc taagggcctc ccctgcttgg ctgggcgcag
     4741 tggctcatgc ctgtaatccc agcactttgg gaggccaagg caggcggatc acgaggttgg
     4801 gagatcgaga ccatcctggc taacggtgaa accccgtctc tactgaaaaa tacaaaaaaa
     4861 aattagccgg gcgtggtgct gggcacctgt agtcccagct actcgggagg ctgaggaagg
     4921 agaatggcgt gaacctgggc ggtggagctt gcagtgagct gagatcacgc cactgcactc
     4981 cagcctgggc gacagagcga gattccatct caaaaaaaaa aaaaaaaggc ctcccctgct
     5041 tgccacaggt ctccccaagg cgcactggcc tcatcttggg cctgtgttat ctcctaggtt
     5101 ggctctgact gtaccaccat ccactacaac tacatgtgta acagttcctg catgggcggc
     5161 atgaaccgga ggcccatcct caccatcatc acactggagg actccaggtc aggagccact
     5221 tgccaccctg cacactggcc tgctgtgccc cagcctctgc ttgcctctga cccctgggcc
     5281 cacctcttac cgatttcttc catactacta cccatccacc tctcatcaca tccccggcgg
     5341 ggaatctcct tactgctccc actcagtttt cttttctctg gctttgggac ctcttaacct
     5401 gtggcttctc ctccacctac ctggagctgg agcttaggct ccagaaagga caagggtggt
     5461 tgggagtaga tggagcctgg ttttttaaat gggacaggta ggacctgatt tccttactgc
     5521 ctcttgcttc tctgtcttct ttcctatcag gacaagaaga cgaagcggtg gaggagacca
     5581 agggtgcagt tatgcctcag attcactttt atcacctttc cttgcctctt tcctagcact
     5641 gcccaacaac accagctcct ctccccagcc aaagaagaaa ccactggatg gagaatattt
     5701 cacccttcag gtactaagtc ttgggacctc ttatcaagtg gaaagtttcc agtctaacac
     5761 tcaaaatgcc gttttcttct tgactgtttt acctgcaatt ggggcatttg ccatcagggg
     5821 gcagtgatgc ctcaaagaca atggctcctg gttgtagcta actaacttca gaacaccaac
     5881 ttataccata atatatattt taaaggacca gaccagcttt caaaaagaaa attgttaaag
     5941 agagcatgaa aatggttcta tgactttgcc tgatacagat gctacttgac ttacgatggt
     6001 gttacttcct gataaactcg tcgtaagttg aaaatattgt aagttgaaaa tggatttaat
     6061 acacctaatc taaggaacat catagcttag cctagcctgc gtcgacaccc ctctggcctc
     6121 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     6181 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     6241 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     6301 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     6361 ctttctgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     6421 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     6481 acctgcagcc accatggctt cgtaccccgg ccatcaacac gcctctgcgt tcgaccaggc
     6541 tgcgcgttct cgcggccata gcaaccgacg tacggcgttg cgccctcgcc ggcagcaaga
     6601 agccacggaa gtccgcccgg agcagaaaat gcccacgcta ctgcgggttt atatagacgg
     6661 tccccacggg atggggaaaa ccaccaccac gcaactgctg gtggccctgg gttcgcgcga
     6721 cgatatcgtc tacgtacccg agccgatgac ttactggcgg gtgctggggg cttccgagac
     6781 aatcgcgaac atctacacca cacaacaccg cctcgaccag ggtgagatat cggccgggga
     6841 cgcggcggtg gtaatgacaa gcgcccagat aacaatgggc atgccttatg ccgtgaccga
     6901 cgccgttctg gctcctcata tcggggggga ggctgggagc tcacatgccc cgcccccggc
     6961 cctcaccctc atcttcgacc gccatcccat cgccgccctc ctgtgctacc cggccgcgcg
     7021 gtaccttatg ggcagcatga ccccccaggc cgtgctggcg ttcgtggccc tcatcccgcc
     7081 gaccttgccc ggcaccaaca tcgtgcttgg ggcccttccg gaggacagac acatcgaccg
     7141 cctggccaaa cgccagcgcc ccggcgagcg gctggacctg gctatgctgg ctgcgattcg
     7201 ccgcgtttac gggctacttg ccaatacggt gcggtatctg cagtgcggcg ggtcgtggcg
     7261 ggaggactgg ggacagcttt cggggacggc cgtgccgccc cagggtgccg agccccagag
     7321 caacgcgggc ccacgacccc atatcgggga cacgttattt accctgtttc gggcccccga
     7381 gttgctggcc cccaacggcg acctgtataa cgtgtttgcc tgggccttgg acgtcttggc
     7441 caaacgcctc cgttccatgc acgtctttat cctggattac gaccaatcgc ccgccggctg
     7501 ccgggacgcc ctgctgcaac ttacctccgg gatggtccag acccacgtca ccacccccgg
     7561 ctccataccg acgatatgcg acctggcgcg cacgtttgcc cgggagatgg gggaggctaa
     7621 ctgaaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7681 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7741 atcttatcat gtctggatat caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7801 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7861 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7921 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7981 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8041 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8101 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8161 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8221 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8281 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8341 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8401 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8521 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8581 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8641 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8701 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8821 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8881 gatccccgcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact
     8941 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact
     9001 cagcaaaagt tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg
     9061 ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     9121 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     9181 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     9241 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     9301 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     9361 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     9421 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     9481 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     9541 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc
     9601 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     9661 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     9721 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     9781 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     9841 atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg
     9901 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
     9961 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaattc atcgatgatg
    10021 gttgagatgt gtataagaga cagattattg aagcatttat cagggttatt gtctcatgag
    10081 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10141 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10201 taggcgtatc acgaggccct ttcgtc
//
