LOCUS       sequence-457019-        7091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse EHMT1 with deletion of aa 809-1298.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Biligiri KK, Sharma NR, Mohanty A, Sarkar DP, Vemula PK, Rampalli S.
  TITLE     A cytoplasmic form of EHMT1N methylates viral proteins to enable 
            inclusion body maturation and efficient viral replication
  JOURNAL   PLOS Biology 22(11): e3002871.
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLOS 
            Biology 22(11): e3002871."
COMMENT     Sequence Label: EGFP_EHMT1B1-4
FEATURES             Location/Qualifiers
     source          1..7091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1757..1878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1794..1813)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1848..1867
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1885..2340)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2022..2043)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2234..2253
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2367..2471
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2473..2830
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2473..2664
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2496..2516)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2681..2816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2865..3659
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2865..3659
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2919..2938)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3529..3548
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3847..3866)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3891..3938
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4267..4855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4756..4775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5030..5333
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5334..5537
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5488..5508
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5576..5585
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5582..5584,5585..5587,5588..6298)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5582..5584,5585..5587,5588..6298)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5627..5648)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5888..5907)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6235..6256
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      6314..6323
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tacctcagaa tcagtgctac atggccacca caaagtccca gacagcagct gcagtatctc
       61 ggaagaaaaa acgaagaatg ggaacctata gtttagttcc caagaaaaag accaaagtat
      121 taaaacagag gacggtgatt gagatgttta aaagcataac ccattccact gtgggcgcca
      181 agggcgagaa agccttagat gatagtgccc tgcatgtaaa tggcgagagc ttggagatgg
      241 actcagaaga tgaagactcc gatgagctgg aggatgacga ggaccatgga gctgagcaag
      301 cggctgcatt tcccaccgag gatagtagga cttctaaaga gagcatgtct gagactgacc
      361 gggctgcaaa gatggatgga gattcagagg aggaacagga gtctcccgac acaggggagg
      421 atgaagatgg tggagacgag tctgacctga gttctgaatc cagtatcaag aagaaatttc
      481 tcaagaggag agggaagact gacagcccct ggatcaaacc tgctcggaaa aggaggcgga
      541 gaagtagaaa gaagccgagc agcatgcttg gttccgaggc ttgtaagtca tctccaggaa
      601 gcatggagca ggcagctctg ggagacagcg ctggctatat ggaagtttcc ctggattccc
      661 tggatctccg tgtcagagga attctgtctt cccagacaga aaatgaaggg ctggccagtg
      721 gtccggatgt gctggggacg gatggcctcc aggaagtgcc tctctgcagc tgccgaatgg
      781 aaacccccaa gagccgcgag atcagcaccc tggccaacaa ccagtgcatg gccactgaga
      841 gcgtggatca cgaaggtaac ttcatggaat gccagcctga gagcagcatc tctcatcgtt
      901 tccataagga ctgtgcctct cgagtcaaca atgccagcta ctgtccccat tgtggggaag
      961 aagcttccaa ggccaaagag gtgaccatag caaaagcaga cacaacttcc acagtgaccc
     1021 tagcccctgg acaggagaag agcctggctg ctgaaggcag ggctgacacg accacgggca
     1081 gcattgctgg agccccagag gatgaaagat cgcagagcac agccccccag gcaccagagt
     1141 gcttcgaccc tgccggaccg gctgggctcg tgaggccgac atctggcctt tcccagggcc
     1201 caggaaagga aaccttggaa agtgctctaa tcgctctaga ctctgaaaaa cccaagaaac
     1261 ttcgcttcca cccaaagcag ctgtacttct ctgccaggca gggtgagctg cagaaggtgc
     1321 ttctcatgct ggttgatgga attgatccca acttcaaaat ggagcaccaa agtaagcgtt
     1381 ccccattaca tgctgctgcg gaggctggcc acgtggacat ctgccacatg ctggttcagg
     1441 cgggtgccaa tattgacact tgctcagagg accagcggac cccactgatg gaggctgcag
     1501 agaacaacca cttggatgca gtgaagtacc tcatcaaggc tggagcacag gtggatccga
     1561 aggacgcaga gggctccaca tgtttgcatt tggctgccaa gaaaggccac gaggtaccgc
     1621 gggcccggga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
     1681 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1741 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1801 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1861 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     1921 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     1981 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     2041 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     2101 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     2161 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     2221 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     2281 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     2341 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2401 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2461 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     2521 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2581 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2641 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     2701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2761 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2821 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     2881 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     2941 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     3001 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     3061 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     3121 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     3181 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     3241 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     3301 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     3361 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     3421 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     3481 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     3541 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     3601 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     3661 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     3721 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     3781 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     3841 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     3901 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     3961 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     4021 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     4081 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     4141 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4201 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4381 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4621 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat
     4981 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     5041 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     5101 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     5161 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     5221 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     5281 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     5341 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     5401 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     5461 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     5521 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     5581 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5641 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5701 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5761 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     5821 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5881 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5941 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     6001 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     6061 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     6121 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     6181 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     6241 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     6301 cggactcaga tctgccacca tggccgccgc tgatgctgag caggcagttc tggccaagca
     6361 agagaccaag caggattgct gcatgaaaac tgagctgcta agggaagata cacctatggc
     6421 tgctgatgaa ggttccacag agaaacaaga aggagagact cccatggctg cagatggaga
     6481 aacaaatggg tcttgtgaaa agagtgggga tcccagccat ctaaatgcac ccaaacacac
     6541 tcaggagaac acaagagcta gcccacagga aggcaccaac agagtgtctc gggtggcaga
     6601 aaatggggtt tcagaaagag acacagaagt ggggaagcaa aaccatgtca cagctgacga
     6661 cttcatgcag acatctgtca ttggcagcaa tggatatttc ttaaataaac cagccctgca
     6721 ggggcagccg ttgaggactc ccaacattct aacctcctcg cttcctggtc atgctgcaaa
     6781 aactcttcct ggaggagcca gtaaatgcag gactctgagt gcacttcctc agacaccaac
     6841 cacagcaccc actgtgcctg gggaagggag tgcagacaca gaggacagaa agcctacagc
     6901 ctcgggcact gatgtcaggg ttcacagggc acgcaagacc atgccgaagt ccatcttggg
     6961 cctgcatgca gccagcaaag accatagaga agttcaagac cataaggaac caaaagagga
     7021 tatcaacaga aacatttctg aatgtggacg acagcagctt ttaccaacct tcccagccct
     7081 ccaccagtcg c
//
