LOCUS       sequence-457050-        7465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpL, GvpS and GvpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaLSK
FEATURES             Location/Qualifiers
     source          1..7465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5723..5741)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5737..5784
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5851..5942
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6807..6846
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6877..7465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7366..7385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgtg cttcacaaat
     3481 tagtaaccgc acccattaac cttgtagtga aaatcggcga aaaagtacag gaagaagctg
     3541 ataaacagct atatgacctt ccgacgattc agcaaaagct cattcagctt caaatgatgt
     3601 ttgagcttgg tgaaattcca gaagaagcgt ttcaagaaaa agaagatgaa ttgttaatga
     3661 ggtacgaaat tgcgaaacgc agagaaattg aacaatggga agagctaaca caaaaaagaa
     3721 atgaggaatc ctagaccctt gcgagcagtc gtatgctttc tcgaattccg agcggttaag
     3781 cgtgacaggc ggtcagtagg aggaacagta tggcagtcga acataatatg cagtcaagta
     3841 cgattgtaga tgtgctcgaa aagattttgg ataaaggagt cgttatagcg ggggacatca
     3901 ccgtaggaat tgcagatgtc gagctattaa cgataaagat ccgcttgatt gtggcttcgg
     3961 ttgataaggc aaaagaaatc ggcatggact ggtgggaaaa tgatccgtat ctcagttcaa
     4021 aaggagccaa taacaaagcg ctcgaagaag aaaataaaat gctgcatgag cggttaaaaa
     4081 cgcttgaaga aaaaatagaa acgaaacgtt aaaaactgta cgctacttaa aaaatggagg
     4141 gatttacaat ggcaactgaa acaaaattag ataacacaca ggcagaaaac aaggaaaata
     4201 aaaatgcgga aaacggttca aaagaaaaga acggttcaaa agcaagcaaa acaacaagca
     4261 gcgggccaat caaacgagcg gtagcaggag gcatcatcgg tgcaacgatt ggatatgtat
     4321 cgactcctga aaatcgaaaa agtctccttg accgcattga tacagacgaa ttaaaaagca
     4381 aagcatctga tttaggaaca aaggtaaaag aaaaatcaaa aagcagcgtg gccagcctga
     4441 aaacatctgc gggaagcttg tttaaaaaag ataaagataa atcaaaagat gatgaagaaa
     4501 acgtaaattc ttctagtagc gaaacagaag acgataacgt tcaagagtac gacgagttaa
     4561 aagaagaaaa tcaaactctt caagatcgct tatcacagct tgaagaaaaa atgaacatgc
     4621 ttgttgagct tagcctcaat aaaaatcaag acgaagaagc ggaagataca gattccgacg
     4681 aagaagagaa cgatgagaac gatgaaaacg atgaaaacga gcaggacgat gaaaacgaag
     4741 aagaaacatc taagccacgt aaaaaggata aaaaagaagc tgaggaagaa gaaagtgaaa
     4801 gtgacgaaga cagcgaggaa gaagaggaag attctcgctc aaacaaaaaa aataaaaaag
     4861 taaaaacaga agaagaagac gaagatgaaa gcgaagaaga aaaaaaggaa gcgaaaccaa
     4921 aaaagtcaac agctaaaaaa tcaaaaaata caaaagcaaa gaaaaacacg gacgaagaag
     4981 atgatgaagc aacatctctt tctagtgaag acgatacaac agcctaagac gtaaaggagg
     5041 aaagaaagac atgagtacag gcccttcttt ttcaactaaa gacaatacgc ttgaatactt
     5101 tgtgaaagct tctaataaac acggcttttc acttgatatt tcattaaatg taaacggcgc
     5161 tgtgatttcc ggtaccatga tttcagcaaa agaatacttt gattacttaa gcgaaacgtt
     5221 tgaagaaggc agtgaagtgg ctcaggcgct aagcgaacaa ttctctttag caagcgaagc
     5281 gagcgaatca aacggagaag cagaagccca ttttattcat ttgaaaaata caaagattta
     5341 ctgtggagac agtaaatcta ctccttctaa aggcaaaatc ttttggagag ggaaaatagc
     5401 agaagtagac gggtttttct taggaaagat ttctgatgca aaatcaacga gtaaaaagag
     5461 ttcataaaaa acggcggggt gattgccccg ccgtttttta gtgatgtgat gagatgtgca
     5521 gcttcttttt tcgatacatg agcggagtca tcttcatttc ttttcgaaac tgattaataa
     5581 aatagcttgt actattaaac cctacttgat aggcgacttc cgtcacattt gcttctgctt
     5641 gctgcagtcc ggattcgcga acgcgtctcg agagatccgg ctgctaacaa agcccgaaag
     5701 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     5761 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccgacta gtagcccgcc
     5821 taatgagcgg gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata
     5881 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5941 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6001 ctgtttttgc tcacccagaa acgctcgtga aagtaaaaga cgcagaggac caattggggg
     6061 cacgagtggg atacatagaa ctggacttga atagcggtaa aatccttgag agttttcgcc
     6121 ctgaagagcg ttttccaatg atgagcactt tcaaagttct gctatgtgga gcagtattat
     6181 cccgtgtaga tgcggggcaa gagcaactcg gacgacgaat acactattcg cagaatgact
     6241 tggttgaata ctccccagtg acagaaaagc accttacgga cggaatgacg gtaagagaat
     6301 tatgtagtgc cgccataacg atgagtgata acactgcggc gaacttactt ctgacaacca
     6361 tcggtggacc gaaggaatta accgcttttt tgcacaatat gggagaccat gtaactcgcc
     6421 ttgaccgttg ggaaccagaa ctgaatgaag ccataccaaa cgacgagcga gacaccacaa
     6481 tgcctgcggc aatggcaaca acattacgca aactattaac tggcgaacta cttactctgg
     6541 cttcacggca acaattaata gactggcttg aagcggataa agttgcagga ccactactgc
     6601 gttcggcact tcctgctggc tggtttattg ctgataaatc tggggcagga gagcgtggtt
     6661 cacggggtat cattgccgca cttggaccag atggtaagcc ttcccgtatc gtagttatct
     6721 acacgacggg tagtcaggca actatggacg aacgaaatag acagattgct gaaatagggg
     6781 cttcactgat taagcattgg taaaccgata caattaaagg ctccttttgg agcctttttt
     6841 tttggacgga ccggtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6901 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6961 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7021 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7081 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7141 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7201 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7261 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7321 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7381 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7441 gtcagggggg cggagcctat ggaaa
//
