LOCUS       sequence-457057-        7775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB, GvpS and GvpU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBSU
FEATURES             Location/Qualifiers
     source          1..7775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2927..2944
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6033..6051)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6047..6094
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6161..6252
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7117..7156
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7187..7775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7676..7695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcatt agatctaact attggaggct actaaaaatg gaaattaaaa
     1141 aaattatgca agccgtgaac gactttttcg gtgaacacgt agctcctcct cataaaatta
     1201 cctcggtgga agctactgaa gatgaaggtt ggagagttat tgttgaagtc attgaagaac
     1261 gagaatatat gaaaaaatac gccaaagatg aaatgctcgg aacgtacgag tgctttgtaa
     1321 ataaagaaaa agaagtcatt tcattcaaac gactcgacgt cagatataga agcgccattg
     1381 gcattgaagc ataaacgata agatggcagg aggaacgtaa aaatgaccgt cttaacagac
     1441 aaaaggaaaa aaggcagtgg agcttttata caagatgacg agacaaaaga ggttctttca
     1501 agagcgctga gctatttaaa atccggctat tccattcatt ttacaggtcc tgccggcgga
     1561 ggcaaaacct ctttagcgcg agcgcttgct aaaaagagaa agcgtcctgt aatgctgatg
     1621 cacgggaatc acgagctcaa caacaaagat ttaattggcg attttacggg atacacgagc
     1681 aaaaaagtaa tcgaccagta cgttcgttct gtctataaaa aagatgaaca ggtgagtgaa
     1741 aactggcagg atggccgatt gcttgaagct gtaaaaaatg gctatacgct gatttacgac
     1801 gaatttactc gttctaagcc tgcgacgaat aatatctttc tatcgatatt agaagaaggc
     1861 gtgctgccgc tgtatggagt aaaaatgacc gatccttttg tgcgcgtgca tcccgatttc
     1921 cgcgtcatct tcacaagcaa tccagctgag tatgccggcg tatatgatac gcaagatgcg
     1981 cttctcgaca ggttaattac catgtttatt gattataaag acatcgacag agagacagcg
     2041 attttaacgg agaaaacgga cgtagaagaa gatgaagcgc gcacaattgt aacgctcgta
     2101 gcaaacgtgc gaaaccgctc tggagacgaa aacagcagcg gacttagcct gcgggcttcg
     2161 cttatgatcg ctacccttgc cacgcagcaa gacattccta tcgatggaag tgacgaagat
     2221 tttcaaacgt tatgtatcga tattttgcat catccgctta ccaaatgttt ggatgaagaa
     2281 aatgcaaaaa gcaaagccga aaaaatcatt ttagaagaat gtaagaatat agacactgaa
     2341 gaaaagtaaa ggagcttgaa aacatgagtg aaacaaacga aacaggtatt tatattttta
     2401 gcgccattca aacggataaa gacgaagaat ttggcgccgt ggaagtagaa ggaacaaaag
     2461 ctgaaacatt tttgattcgc tacaaagacg cggctatggt agcagctgaa gtaccgatga
     2521 aaatttatca tcctaatcgc caaaatttat taatgcatca aaacgcagta gcagcgatta
     2581 tggacaagaa cgatacggtt attccaatca gctttgggaa tgtattcaaa tcaaaagaag
     2641 acgtaaaagt tcttttggaa aacctttatc cgcagtttga aaagctgttt ccagcgatca
     2701 aaggaaaaat tgaagtcggt ttaaaagtaa ttgggaaaaa agaatggctt gagaaaaaag
     2761 taaacgaaaa tcctgaactt gagaaagtat cagcatccgt aaaaggaaaa tcagaagcag
     2821 ccggttatta tgagcgtatt caacttggag gaatggctca aaagatgttt acttccctgc
     2881 aaaaagaagt caagacagat gtgttttctc cgcttgaaga agcagcggaa gcagcaaaag
     2941 caaatgagcc aacgggcgaa acgatgcttt taaacgcgtc tttcttaatt aaccgagaag
     3001 atgaagcgaa gtttgatgaa aaagtaaatg aagcgcatga aaactggaaa gacaaagccg
     3061 attttcatta cagcggtcct tggcctgctt ataattttgt gaacattcgc ctaaaagtag
     3121 aagagaaata acgtgcttca caaattagta accgcaccca ttaaccttgt agtgaaaatc
     3181 ggcgaaaaag tacaggaaga agctgataaa cagctatatg accttccgac gattcagcaa
     3241 aagctcattc agcttcaaat gatgtttgag cttggtgaaa ttccagaaga agcgtttcaa
     3301 gaaaaagaag atgaattgtt aatgaggtac gaaattgcga aacgcagaga aattgaacaa
     3361 tgggaagagc taacacaaaa aagaaatgag gaatcctaga tgggagaatt actgtattta
     3421 tacggtttaa ttccaacaaa agaagcagca gccatagagc cgtttccatc ttataagggg
     3481 tttgacggag aacattcact gtacccaatt gcgtttgatc aggtgacggc tgtagtttct
     3541 aagctggatg ctgacaccta ttcagaaaaa gtgattcaag aaaaaatgga gcaggatatg
     3601 agctggctgc aggaaaaagc atttcatcat cacgaaacgg tagccgcttt gtacgaagaa
     3661 tttacgatca ttccattaaa attttgcacc atttataaag gtgaagaaag tctgcaggca
     3721 gctattgaga ttaacaaaga aaagatagag aattcactga cgctgcttca aggaaatgaa
     3781 gagtggaatg tgaaaattta ctgtgatgat acagagctta aaaaaggaat cagcgaaacg
     3841 aatgaaagcg tgaaagcgaa aaaacaagaa attagtcact tatcaccagg aagacagttt
     3901 tttgaaaaga aaaaaataga tcagctgatt gaaaaagaat tagagcttca caaaaacaaa
     3961 gtgtgtgaag agatacatga caagctaaaa gaattatcgc tttatgactc tgttaaaaag
     4021 aattggagca aagacgtaac tggcgcagct gaacagatgg cgtggaacag cgtgtttctt
     4081 ctcccgtctc tgcagattac taagttcgta aacgaaatag aagagcttca gcaaaggctt
     4141 gaaaataaag gctggaagtt tgaagtgacg ggaccatggc cgccctatca tttctcgagc
     4201 tttccgtaaa agcggttaag cgtgacagtc caggaggaat taatggatgc aaccggtcag
     4261 ccaagcaaat ggacgaatcc acttggatcc tgatcaagct gaacaaggct tagcgcagct
     4321 tgtgatgaca gttattgagc tattgaggca aatcgttgaa cgtcatgcca tgaggcgggt
     4381 ggagggtgga acgttgacgg acgaacaaat tgaaaactta ggaattgcac taatgaactt
     4441 agaagaaaaa atggacgagt tgaaagaggt gttcggtctg gatgcagaag atttaaatat
     4501 tgatcttgga ccgctaggca gcctgcttta agcggtcagt aggaggaaca gtatggcagt
     4561 cgaacataat atgcagtcaa gtacgattgt agatgtgctc gaaaagattt tggataaagg
     4621 agtcgttata gcgggggaca tcaccgtagg aattgcagat gtcgagctat taacgataaa
     4681 gatccgcttg attgtggctt cggttgataa ggcaaaagaa atcggcatgg actggtggga
     4741 aaatgatccg tatctcagtt caaaaggagc caataacaaa gcgctcgaag aagaaaataa
     4801 aatgctgcat gagcggttaa aaacgcttga agaaaaaata gaaacgaaac gttaaaaact
     4861 gtacgctact taaaaaatgg agggatttac aatggcaact gaaacaaaat tagataacac
     4921 acaggcagaa aacaaggaaa ataaaaatgc ggaaaacggt tcaaaagaaa agaacggttc
     4981 aaaagcaagc aaaacaacaa gcagcgggcc aatcaaacga gcggtagcag gaggcatcat
     5041 cggtgcaacg attggatatg tatcgactcc tgaaaatcga aaaagtctcc ttgaccgcat
     5101 tgatacagac gaattaaaaa gcaaagcatc tgatttagga acaaaggtaa aagaaaaatc
     5161 aaaaagcagc gtggccagcc tgaaaacatc tgcgggaagc ttgtttaaaa aagataaaga
     5221 taaatcaaaa gatgatgaag aaaacgtaaa ttcttctagt agcgaaacag aagacgataa
     5281 cgttcaagag tacgacgagt taaaagaaga aaatcaaact cttcaagatc gcttatcaca
     5341 gcttgaagaa aaaatgaaca tgcttgttga gcttagcctc aataaaaatc aagacgaaga
     5401 agcggaagat acagattccg acgaagaaga gaacgatgag aacgatgaaa acgatgaaaa
     5461 cgagcaggac gatgaaaacg aagaagaaac atctaagcca cgtaaaaagg ataaaaaaga
     5521 agctgaggaa gaagaaagtg aaagtgacga agacagcgag gaagaagagg aagattctcg
     5581 ctcaaacaaa aaaaataaaa aagtaaaaac agaagaagaa gacgaagatg aaagcgaaga
     5641 agaaaaaaag gaagcgaaac caaaaaagtc aacagctaaa aaatcaaaaa atacaaaagc
     5701 aaagaaaaac acggacgaag aagatgatga agcaacatct ctttctagtg aagacgatac
     5761 aacagcctaa gacgtaaaaa acggcggggt gattgccccg ccgtttttta gtgatgtgat
     5821 gagatgtgca gcttcttttt tcgatacatg agcggagtca tcttcatttc ttttcgaaac
     5881 tgattaataa aatagcttgt actattaaac cctacttgat aggcgacttc cgtcacattt
     5941 gcttctgctt gctgcagtcc ggattcgcga acgcgtctcg agagatccgg ctgctaacaa
     6001 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     6061 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccgacta
     6121 gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt tttctaaata
     6181 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6241 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6301 ttttgccttc ctgtttttgc tcacccagaa acgctcgtga aagtaaaaga cgcagaggac
     6361 caattggggg cacgagtggg atacatagaa ctggacttga atagcggtaa aatccttgag
     6421 agttttcgcc ctgaagagcg ttttccaatg atgagcactt tcaaagttct gctatgtgga
     6481 gcagtattat cccgtgtaga tgcggggcaa gagcaactcg gacgacgaat acactattcg
     6541 cagaatgact tggttgaata ctccccagtg acagaaaagc accttacgga cggaatgacg
     6601 gtaagagaat tatgtagtgc cgccataacg atgagtgata acactgcggc gaacttactt
     6661 ctgacaacca tcggtggacc gaaggaatta accgcttttt tgcacaatat gggagaccat
     6721 gtaactcgcc ttgaccgttg ggaaccagaa ctgaatgaag ccataccaaa cgacgagcga
     6781 gacaccacaa tgcctgcggc aatggcaaca acattacgca aactattaac tggcgaacta
     6841 cttactctgg cttcacggca acaattaata gactggcttg aagcggataa agttgcagga
     6901 ccactactgc gttcggcact tcctgctggc tggtttattg ctgataaatc tggggcagga
     6961 gagcgtggtt cacggggtat cattgccgca cttggaccag atggtaagcc ttcccgtatc
     7021 gtagttatct acacgacggg tagtcaggca actatggacg aacgaaatag acagattgct
     7081 gaaatagggg cttcactgat taagcattgg taaaccgata caattaaagg ctccttttgg
     7141 agcctttttt tttggacgga ccggtagaaa agatcaaagg atcttcttga gatccttttt
     7201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7321 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7741 tgtgatgctc gtcagggggg cggagcctat ggaaa
//
