LOCUS       sequence-457058-        7872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB, GvpR and GvpG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBRG
FEATURES             Location/Qualifiers
     source          1..7872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             670..687
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(4180..4198)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4194..4241
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4308..4399
                     /gene="bla"
                     /label=AmpR promoter
     terminator      5264..5303
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      5334..5922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5823..5842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6076..6093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6108..6247
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6173..6192)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6333..6355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6349..6540)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     6777..6796
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6777..6795
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttaccaaatg tttggatgaa gaaaatgcaa aaagcaaagc cgaaaaaatc attttagaag
       61 aatgtaagaa tatagacact gaagaaaagt aaaggagctt gaaaacatga gtgaaacaaa
      121 cgaaacaggt atttatattt ttagcgccat tcaaacggat aaagacgaag aatttggcgc
      181 cgtggaagta gaaggaacaa aagctgaaac atttttgatt cgctacaaag acgcggctat
      241 ggtagcagct gaagtaccga tgaaaattta tcatcctaat cgccaaaatt tattaatgca
      301 tcaaaacgca gtagcagcga ttatggacaa gaacgatacg gttattccaa tcagctttgg
      361 gaatgtattc aaatcaaaag aagacgtaaa agttcttttg gaaaaccttt atccgcagtt
      421 tgaaaagctg tttccagcga tcaaaggaaa aattgaagtc ggtttaaaag taattgggaa
      481 aaaagaatgg cttgagaaaa aagtaaacga aaatcctgaa cttgagaaag tatcagcatc
      541 cgtaaaagga aaatcagaag cagccggtta ttatgagcgt attcaacttg gaggaatggc
      601 tcaaaagatg tttacttccc tgcaaaaaga agtcaagaca gatgtgtttt ctccgcttga
      661 agaagcagcg gaagcagcaa aagcaaatga gccaacgggc gaaacgatgc ttttaaacgc
      721 gtctttctta attaaccgag aagatgaagc gaagtttgat gaaaaagtaa atgaagcgca
      781 tgaaaactgg aaagacaaag ccgattttca ttacagcggt ccttggcctg cttataattt
      841 tgtgaacatt cgcctaaaag tagaagagaa ataacgaaat gaggaatcct agatgggaga
      901 attactgtat ttatacggtt taattccaac aaaagaagca gcagccatag agccgtttcc
      961 atcttataag gggtttgacg gagaacattc actgtaccca attgcgtttg atcaggtgac
     1021 ggctgtagtt tctaagctgg atgctgacac ctattcagaa aaagtgattc aagaaaaaat
     1081 ggagcaggat atgagctggc tgcaggaaaa agcatttcat catcacgaaa cggtagccgc
     1141 tttgtacgaa gaatttacga tcattccatt aaaattttgc accatttata aaggtgaaga
     1201 aagtctgcag gcagctattg agattaacaa agaaaagata gagaattcac tgacgctgct
     1261 tcaaggaaat gaagagtgga atgtgaaaat ttactgtgat gatacagagc ttaaaaaagg
     1321 aatcagcgaa acgaatgaaa gcgtgaaagc gaaaaaacaa gaaattagtc acttatcacc
     1381 aggaagacag ttttttgaaa agaaaaaaat agatcagctg attgaaaaag aattagagct
     1441 tcacaaaaac aaagtgtgtg aagagataca tgacaagcta aaagaattat cgctttatga
     1501 ctctgttaaa aagaattgga gcaaagacgt aactggcgca gctgaacaga tggcgtggaa
     1561 cagcgtgttt cttctcccgt ctctgcagat tactaagttc gtaaacgaaa tagaagagct
     1621 tcagcaaagg cttgaaaata aaggctggaa gtttgaagtg acgggaccat ggccgcccta
     1681 tcatttctcg agctttgcgt aaagtgagga attaacatta tgtctcttaa acaatccatg
     1741 gagaataaag atattgctct tattgatatt ttagatgtca ttttagataa aggagtcgcc
     1801 attaaaggag acttaatcat ttccatagct ggcgtcgatt tagtgtattt ggatttgcgg
     1861 gtgcttattt cttcggttga aacgcttgtg caagcaaaag aaggaaatca caaaccaatc
     1921 acttctgaac aatttgataa acaaaaggag gaattaatgg atgcaaccgg tcagccaagc
     1981 aaatggacga atccacttgg atcctgatca agctgaacaa ggcttagcgc agcttgtgat
     2041 gacagttatt gagctattga ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg
     2101 tggaacgttg acggacgaac aaattgaaaa cttaggaatt gcactaatga acttagaaga
     2161 aaaaatggac gagttgaaag aggtgttcgg tctggatgca gaagatttaa atattgatct
     2221 tggaccgcta ggcagcctgc tttaagcggt cagtaggagg aacagtatgg cagtcgaaca
     2281 taatatgcag tcaagtacga ttgtagatgt gctcgaaaag attttggata aaggagtcgt
     2341 tatagcgggg gacatcaccg taggaattgc agatgtcgag ctattaacga taaagatccg
     2401 cttgattgtg gcttcggttg ataaggcaaa agaaatcggc atggactggt gggaaaatga
     2461 tccgtatctc agttcaaaag gagccaataa caaagcgctc gaagaagaaa ataaaatgct
     2521 gcatgagcgg ttaaaaacgc ttgaagaaaa aatagaaacg aaacgttaaa aactgtacgc
     2581 tacttaaaaa atggagggat ttacaatggc aactgaaaca aaattagata acacacaggc
     2641 agaaaacaag gaaaataaaa atgcggaaaa cggttcaaaa gaaaagaacg gttcaaaagc
     2701 aagcaaaaca acaagcagcg ggccaatcaa acgagcggta gcaggaggca tcatcggtgc
     2761 aacgattgga tatgtatcga ctcctgaaaa tcgaaaaagt ctccttgacc gcattgatac
     2821 agacgaatta aaaagcaaag catctgattt aggaacaaag gtaaaagaaa aatcaaaaag
     2881 cagcgtggcc agcctgaaaa catctgcggg aagcttgttt aaaaaagata aagataaatc
     2941 aaaagatgat gaagaaaacg taaattcttc tagtagcgaa acagaagacg ataacgttca
     3001 agagtacgac gagttaaaag aagaaaatca aactcttcaa gatcgcttat cacagcttga
     3061 agaaaaaatg aacatgcttg ttgagcttag cctcaataaa aatcaagacg aagaagcgga
     3121 agatacagat tccgacgaag aagagaacga tgagaacgat gaaaacgatg aaaacgagca
     3181 ggacgatgaa aacgaagaag aaacatctaa gccacgtaaa aaggataaaa aagaagctga
     3241 ggaagaagaa agtgaaagtg acgaagacag cgaggaagaa gaggaagatt ctcgctcaaa
     3301 caaaaaaaat aaaaaagtaa aaacagaaga agaagacgaa gatgaaagcg aagaagaaaa
     3361 aaaggaagcg aaaccaaaaa agtcaacagc taaaaaatca aaaaatacaa aagcaaagaa
     3421 aaacacggac gaagaagatg atgaagcaac atctctttct agtgaagacg atacaacagc
     3481 ctaagacgta aaggaggaaa gaaagacatg agtacaggcc cttctttttc aactaaagac
     3541 aatacgcttg aatactttgt gaaagcttct aataaacacg gcttttcact tgatatttca
     3601 ttaaatgtaa acggcgctgt gatttccggt accatgattt cagcaaaaga atactttgat
     3661 tacttaagcg aaacgtttga agaaggcagt gaagtggctc aggcgctaag cgaacaattc
     3721 tctttagcaa gcgaagcgag cgaatcaaac ggagaagcag aagcccattt tattcatttg
     3781 aaaaatacaa agatttactg tggagacagt aaatctactc cttctaaagg caaaatcttt
     3841 tggagaggga aaatagcaga agtagacggg tttttcttag gaaagatttc tgatgcaaaa
     3901 tcaacgagta aaaagagttc ataaaaaacg gcggggtgat tgccccgccg ttttttagtg
     3961 atgtgatgag atgtgcagct tcttttttcg atacatgagc ggagtcatct tcatttcttt
     4021 tcgaaactga ttaataaaat agcttgtact attaaaccct acttgatagg cgacttccgt
     4081 cacatttgct tctgcttgct gcagtccgga ttcgcgaacg cgtctcgaga gatccggctg
     4141 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     4201 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     4261 ccgactagta gcccgcctaa tgagcgggct tttttttaat tcccctattt gtttattttt
     4321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4381 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4441 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctcgtgaaag taaaagacgc
     4501 agaggaccaa ttgggggcac gagtgggata catagaactg gacttgaata gcggtaaaat
     4561 ccttgagagt tttcgccctg aagagcgttt tccaatgatg agcactttca aagttctgct
     4621 atgtggagca gtattatccc gtgtagatgc ggggcaagag caactcggac gacgaataca
     4681 ctattcgcag aatgacttgg ttgaatactc cccagtgaca gaaaagcacc ttacggacgg
     4741 aatgacggta agagaattat gtagtgccgc cataacgatg agtgataaca ctgcggcgaa
     4801 cttacttctg acaaccatcg gtggaccgaa ggaattaacc gcttttttgc acaatatggg
     4861 agaccatgta actcgccttg accgttggga accagaactg aatgaagcca taccaaacga
     4921 cgagcgagac accacaatgc ctgcggcaat ggcaacaaca ttacgcaaac tattaactgg
     4981 cgaactactt actctggctt cacggcaaca attaatagac tggcttgaag cggataaagt
     5041 tgcaggacca ctactgcgtt cggcacttcc tgctggctgg tttattgctg ataaatctgg
     5101 ggcaggagag cgtggttcac ggggtatcat tgccgcactt ggaccagatg gtaagccttc
     5161 ccgtatcgta gttatctaca cgacgggtag tcaggcaact atggacgaac gaaatagaca
     5221 gattgctgaa ataggggctt cactgattaa gcattggtaa accgatacaa ttaaaggctc
     5281 cttttggagc cttttttttt ggacggaccg gtagaaaaga tcaaaggatc ttcttgagat
     5341 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5401 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5461 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5521 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5581 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5641 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5701 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5761 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5821 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5881 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5941 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6001 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6061 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     6121 tttctcctta cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct
     6181 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     6241 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     6361 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     6421 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     6481 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     6541 tgatgcctcc gtgtaagggg gatttctgtt catggggtaa tgataccgat gaaacgagag
     6601 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
     6661 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
     6721 cagcgcttcg ttaatacaga tgtaggtgtt ggcaccatgc atcgatcccg cgaaattaat
     6781 acgactcact atagggagac cacaacggtt tccctctaga gatagcttag cgatgtgaaa
     6841 gaagctggag aaaactttca agaaaaagtt tcttcttcag acgataaaga gaagtcaaaa
     6901 aataagcgga aaataaaagg cgtaaatcag attaaaaaag caacggatat taaaagctct
     6961 actaagatta aaagttctaa tgatattaaa tcatctacag atttaaaaac aatgggatca
     7021 tcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     7081 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     7141 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     7201 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     7261 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     7321 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     7381 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     7441 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     7501 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     7561 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     7621 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     7681 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     7741 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     7801 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     7861 tgcatcatcc gc
//
