LOCUS       sequence-457063-        2701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for optimized 2xHA-TEAD inhibitor (TEADiv2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Branch B, Yuan Y, Cascone M, Raimondi F, Iglesias-Bartolome R
  TITLE     An improved TEAD dominant-negative protein inhibitor to study Hippo 
            YAP1/TAZ-dependent transcription.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 3:2024.10.03.615022. doi: 
            10.1101/2024.10.03.615022.
  PUBMED    39502361
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 3:2024.10.03.615022. doi: 
            10.1101/2024.10.03.615022."
COMMENT     Sequence Label: pENTR-HA-TEADiv2
FEATURES             Location/Qualifiers
     source          1..2701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     384..403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      648..734
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      826..853
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     861..884
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    903..1002
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1059..1085
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1089..1115
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1410..1430
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1475..1574)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1686..1705)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1697..2506
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1764..1783)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(2596..2701,1..483)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
       61 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      121 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      181 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      241 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      301 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      361 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      421 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      481 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      541 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc tagcatggat
      601 ctcggggacg tctaactact aagcgagagt agggaactgc caggcatcaa ataaaacgaa
      661 aggctcagtc ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
      721 tgagtaggac aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg cccggagggt
      781 ggcgggcagg acgcccgcca taaactgcca ggcatcaaac taagcagaag gccatcctga
      841 cggatggcct ttttgcgttt ctacaaactc ttcctgttag ttagttactt aagctcgggc
      901 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc
      961 aatgcttttt tataatgcca actttgtaca aaaaagcagg ctctttaaag gaaccaattc
     1021 agtcgacggc cattgtggcc atcaagcttg ccatggccta cccatacgat gttcctgact
     1081 atgcgggcta tccctatgac gtcccggact atgcaggatc ctcagtagac gaccactttg
     1141 cgaaagcact gggtgataca tggctgcaaa taggtgaccc ccctgtcgcg accaatccca
     1201 aaacggccaa tgttccgcaa acagtgccca tgcggttgcg gaagttgccg gagtcttttt
     1261 tcaaagagcc cgagggagac cctccagtgg cgacgaaccc caaaccctct tcatggcgaa
     1321 aaaagattct gcccgagagc ttttttaaag aaccggggga cccgccagtt gcgacaaagc
     1381 gaacagcgga tggcagtgag ttcgagtccc cgaagaaaaa gcgcaaggtt gagtaagcgg
     1441 ccgcactcga gatatcggcc tccagggcct ctagacccag ctttcttgta caaagttggc
     1501 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1561 aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc tctgatgtta
     1621 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1681 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     1741 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1801 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1861 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     1921 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1981 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2041 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2101 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2161 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2221 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     2281 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     2341 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     2401 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     2461 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     2521 ttggttgtaa cattattcag attgggcccc gttccactga gcgtcagacc ccgtagaaaa
     2581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2701 g
//
