LOCUS       sequence-457065-        7192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpL and GvpT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaLT
FEATURES             Location/Qualifiers
     source          1..7192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5450..5468)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5464..5511
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5578..5669
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6534..6573
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6604..7192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7093..7112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgtg cttcacaaat
     3481 tagtaaccgc acccattaac cttgtagtga aaatcggcga aaaagtacag gaagaagctg
     3541 ataaacagct atatgacctt ccgacgattc agcaaaagct cattcagctt caaatgatgt
     3601 ttgagcttgg tgaaattcca gaagaagcgt ttcaagaaaa agaagatgaa ttgttaatga
     3661 ggtacgaaat tgcgaaacgc agagaaattg aacaatggga agagctaaca caaaaaagaa
     3721 atgaggaatc ctagagtgag gaattaacat tatgtctctt aaacaatcca tggagaataa
     3781 agatattgct cttattgata ttttagatgt cattttagat aaaggagtcg ccattaaagg
     3841 agacttaatc atttccatag ctggcgtcga tttagtgtat ttggatttgc gggtgcttat
     3901 ttcttcggtt gaaacgcttg tgcaagcaaa agaaggaaat cacaaaccaa tcacttctga
     3961 acaatttgat aaacaaaaag aagagctgat ggacgctacg ggccagccta gtaagtggac
     4021 gaaccctcta ggttcgtgac ccttgcgagc agtcgtatgc tttctcgaat tccgagcggt
     4081 taagcgtgac agtccaggag gaattaatag atgcaaccgg tcagccaagc aaatggacga
     4141 atccacttgg atcctgatca agctgaacaa ggcttagcgc agcttgtgat gacagttatt
     4201 gagctattga ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg tggaacgttg
     4261 acggacgaac aaattgaaaa cttaggaatt gcactaatga acttagaaga aaaaatggac
     4321 gagttgaaag aggtgttcgg tctggatgca gaagatttaa atattgatct tggcccgcta
     4381 ggcagcctgc tttaagcggt cagtaggagg aacagtatgg cagtcgaaca taatatgcag
     4441 tcaagtacga ttgtagatgt gctcgaaaag attttggata aaggagtcgt tatagcgggg
     4501 gacatcaccg taggaattgc agatgtcgag ctattaacga taaagatccg cttgattgtg
     4561 gcttcggttg ataaggcaaa agaaatcggc atggactggt gggaaaatga tccgtatctc
     4621 agttcaaaag gagccaataa caaagcgctc gaagaagaaa ataaaatgct gcatgagcgg
     4681 ttaaaaacgc ttgaagaaaa aatagaaacg aaacgttaaa aactgtacgc tacttaaaaa
     4741 atggagggat ttacgacgta aaggaggaaa gaaagacatg agtacaggcc cttctttttc
     4801 aactaaagac aatacgcttg aatactttgt gaaagcttct aataaacacg gcttttcact
     4861 tgatatttca ttaaatgtaa acggcgctgt gatttccggt accatgattt cagcaaaaga
     4921 atactttgat tacttaagcg aaacgtttga agaaggcagt gaagtggctc aggcgctaag
     4981 cgaacaattc tctttagcaa gcgaagcgag cgaatcaaac ggagaagcag aagcccattt
     5041 tattcatttg aaaaatacaa agatttactg tggagacagt aaatctactc cttctaaagg
     5101 caaaatcttt tggagaggga aaatagcaga agtagacggg tttttcttag gaaagatttc
     5161 tgatgcaaaa tcaacgagta aaaagagttc ataaaaaacg gcggggtgat tgccccgccg
     5221 ttttttagtg atgtgatgag atgtgcagct tcttttttcg atacatgagc ggagtcatct
     5281 tcatttcttt tcgaaactga ttaataaaat agcttgtact attaaaccct acttgatagg
     5341 cgacttccgt cacatttgct tctgcttgct gcagtccgga ttcgcgaacg cgtctcgaga
     5401 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     5461 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     5521 ggaactatat ccgactagta gcccgcctaa tgagcgggct tttttttaat tcccctattt
     5581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5641 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5701 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctcgtgaaag
     5761 taaaagacgc agaggaccaa ttgggggcac gagtgggata catagaactg gacttgaata
     5821 gcggtaaaat ccttgagagt tttcgccctg aagagcgttt tccaatgatg agcactttca
     5881 aagttctgct atgtggagca gtattatccc gtgtagatgc ggggcaagag caactcggac
     5941 gacgaataca ctattcgcag aatgacttgg ttgaatactc cccagtgaca gaaaagcacc
     6001 ttacggacgg aatgacggta agagaattat gtagtgccgc cataacgatg agtgataaca
     6061 ctgcggcgaa cttacttctg acaaccatcg gtggaccgaa ggaattaacc gcttttttgc
     6121 acaatatggg agaccatgta actcgccttg accgttggga accagaactg aatgaagcca
     6181 taccaaacga cgagcgagac accacaatgc ctgcggcaat ggcaacaaca ttacgcaaac
     6241 tattaactgg cgaactactt actctggctt cacggcaaca attaatagac tggcttgaag
     6301 cggataaagt tgcaggacca ctactgcgtt cggcacttcc tgctggctgg tttattgctg
     6361 ataaatctgg ggcaggagag cgtggttcac ggggtatcat tgccgcactt ggaccagatg
     6421 gtaagccttc ccgtatcgta gttatctaca cgacgggtag tcaggcaact atggacgaac
     6481 gaaatagaca gattgctgaa ataggggctt cactgattaa gcattggtaa accgatacaa
     6541 ttaaaggctc cttttggagc cttttttttt ggacggaccg gtagaaaaga tcaaaggatc
     6601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6721 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     6781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aa
//
