LOCUS       sequence-457066-        7502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpR, GvpL and GvpS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaRLS
FEATURES             Location/Qualifiers
     source          1..7502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..109
                     /gene="bla"
                     /label=AmpR promoter
     terminator      974..1013
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1044..1632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1533..1552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1786..1803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1818..1957
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1883..1902)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2043..2065
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2059..2250)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2487..2506
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2487..2505
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4628..4645
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(7392..7410)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7406..7453
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
       61 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      121 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      181 cccagaaacg ctcgtgaaag taaaagacgc agaggaccaa ttgggggcac gagtgggata
      241 catagaactg gacttgaata gcggtaaaat ccttgagagt tttcgccctg aagagcgttt
      301 tccaatgatg agcactttca aagttctgct atgtggagca gtattatccc gtgtagatgc
      361 ggggcaagag caactcggac gacgaataca ctattcgcag aatgacttgg ttgaatactc
      421 cccagtgaca gaaaagcacc ttacggacgg aatgacggta agagaattat gtagtgccgc
      481 cataacgatg agtgataaca ctgcggcgaa cttacttctg acaaccatcg gtggaccgaa
      541 ggaattaacc gcttttttgc acaatatggg agaccatgta actcgccttg accgttggga
      601 accagaactg aatgaagcca taccaaacga cgagcgagac accacaatgc ctgcggcaat
      661 ggcaacaaca ttacgcaaac tattaactgg cgaactactt actctggctt cacggcaaca
      721 attaatagac tggcttgaag cggataaagt tgcaggacca ctactgcgtt cggcacttcc
      781 tgctggctgg tttattgctg ataaatctgg ggcaggagag cgtggttcac ggggtatcat
      841 tgccgcactt ggaccagatg gtaagccttc ccgtatcgta gttatctaca cgacgggtag
      901 tcaggcaact atggacgaac gaaatagaca gattgctgaa ataggggctt cactgattaa
      961 gcattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt ggacggaccg
     1021 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1081 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1141 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     1201 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1261 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1321 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1381 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1441 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1501 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1561 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1621 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1681 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1741 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1801 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     1861 caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     1921 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     1981 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     2041 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     2101 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     2161 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     2221 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     2281 catggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     2341 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     2401 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     2461 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
     2521 tccctctaga gatagcttag cgatgtgaaa gaagctggag aaaactttca agaaaaagtt
     2581 tcttcttcag acgataaaga gaagtcaaaa aataagcgga aaataaaagg cgtaaatcag
     2641 attaaaaaag caacggatat taaaagctct actaagatta aaagttctaa tgatattaaa
     2701 tcatctacag atttaaaaac aatgggatca taagcgaaag gagaaatgag atatgtctat
     2761 tcaaaaaagt actaatagtt caagtttagc agaagtcatt gaccgtattt tagataaagg
     2821 aattgttatt gatgcttttg caagagtttc tgttgtagga attgaaattt taacgattga
     2881 agcgcgagtg gttattgcca gtgttgatac atggttacgc tatgcagaag cagtagggct
     2941 tcttcgtgac gacgtagaag aaaacggtct tcctgaacgt tcaaattcaa gtgaagggca
     3001 gccgcgtttt agtatttaat atgaagtggt atggaacctt ccttttttga gggtggttcc
     3061 agcttttaga tctaactatt ggaggctact aaaaatggaa aagatggcag gaggaacgta
     3121 aaaatgaccg tcttaacaga caaaaggaaa aaaggcagtg gagcttttat acaagatgac
     3181 gagacaaaag aggttctttc aagagcgctg agctatttaa aatccggcta ttccattcat
     3241 tttacaggtc ctgccggcgg aggcaaaacc tctttagcgc gagcgcttgc taaaaagaga
     3301 aagcgtcctg taatgctgat gcacgggaat cacgagctca acaacaaaga tttaattggc
     3361 gattttacgg gatacacgag caaaaaagta atcgaccagt acgttcgttc tgtctataaa
     3421 aaagatgaac aggtgagtga aaactggcag gatggccgat tgcttgaagc tgtaaaaaat
     3481 ggctatacgc tgatttacga cgaatttact cgttctaagc ctgcgacgaa taatatcttt
     3541 ctatcgatat tagaagaagg cgtgctgccg ctgtatggag taaaaatgac cgatcctttt
     3601 gtgcgcgtgc atcccgattt ccgcgtcatc ttcacaagca atccagctga gtatgccggc
     3661 gtatatgata cgcaagatgc gcttctcgac aggttaatta ccatgtttat tgattataaa
     3721 gacatcgaca gagagacagc gattttaacg gagaaaacgg acgtagaaga agatgaagcg
     3781 cgcacaattg taacgctcgt agcaaacgtg cgaaaccgct ctggagacga aaacagcagc
     3841 ggacttagcc tgcgggcttc gcttatgatc gctacccttg ccacgcagca agacattcct
     3901 atcgatggaa gtgacgaaga ttttcaaacg ttatgtatcg atattttgca tcatccgctt
     3961 accaaatgtt tggatgaaga aaatgcaaaa agcaaagccg aaaaaatcat tttagaagaa
     4021 tgtaagaata tagacactga agaaaagtaa aggagcttga aaacatgagt gaaacaaacg
     4081 aaacaggtat ttatattttt agcgccattc aaacggataa agacgaagaa tttggcgccg
     4141 tggaagtaga aggaacaaaa gctgaaacat ttttgattcg ctacaaagac gcggctatgg
     4201 tagcagctga agtaccgatg aaaatttatc atcctaatcg ccaaaattta ttaatgcatc
     4261 aaaacgcagt agcagcgatt atggacaaga acgatacggt tattccaatc agctttggga
     4321 atgtattcaa atcaaaagaa gacgtaaaag ttcttttgga aaacctttat ccgcagtttg
     4381 aaaagctgtt tccagcgatc aaaggaaaaa ttgaagtcgg tttaaaagta attgggaaaa
     4441 aagaatggct tgagaaaaaa gtaaacgaaa atcctgaact tgagaaagta tcagcatccg
     4501 taaaaggaaa atcagaagca gccggttatt atgagcgtat tcaacttgga ggaatggctc
     4561 aaaagatgtt tacttccctg caaaaagaag tcaagacaga tgtgttttct ccgcttgaag
     4621 aagcagcgga agcagcaaaa gcaaatgagc caacgggcga aacgatgctt ttaaacgcgt
     4681 ctttcttaat taaccgagaa gatgaagcga agtttgatga aaaagtaaat gaagcgcatg
     4741 aaaactggaa agacaaagcc gattttcatt acagcggtcc ttggcctgct tataattttg
     4801 tgaacattcg cctaaaagta gaagagaaat aacgtgcttc acaaattagt aaccgcaccc
     4861 attaaccttg tagtgaaaat cggcgaaaaa gtacaggaag aagctgataa acagctatat
     4921 gaccttccga cgattcagca aaagctcatt cagcttcaaa tgatgtttga gcttggtgaa
     4981 attccagaag aagcgtttca agaaaaagaa gatgaattgt taatgaggta cgaaattgcg
     5041 aaacgcagag aaattgaaca atgggaaggg ctaacacaaa aaagaaatga ggaatcctag
     5101 acccttgcga gcagtcgtat gctttctcga attccgagcg gttaagcgtg acagtccagg
     5161 aggaattaat agatgcaacc ggtcagccaa gcaaatggac gaatccactt ggatcctgat
     5221 caagctgaac aaggcttagc gcagcttgtg atgacagtta ttgagctatt gaggcaaatc
     5281 gttgaacgtc atgccatgag gcgggtggag ggtggaacgt tgacggacga acaaattgaa
     5341 aacttaggaa ttgcactaat gaacttagaa gaaaaaatgg acgagttgaa agaggtgttc
     5401 ggtctggatg cagaagattt aaatattgat cttggcccgc taggcagcct gctttaagcg
     5461 gtcagtagga ggaacagtat ggcagtcgaa cataatatgc agtcaagtac gattgtagat
     5521 gtgctcgaaa agattttgga taaaggagtc gttatagcgg gggacatcac cgtaggaatt
     5581 gcagatgtcg agctattaac gataaagatc cgcttgattg tggcttcggt tgataaggca
     5641 aaagaaatcg gcatggactg gtgggaaaat gatccgtatc tcagttcaaa aggagccaat
     5701 aacaaagcgc tcgaagaaga aaataaaatg ctgcatgagc ggttaaaaac gcttgaagaa
     5761 aaaatagaaa cgaaacgtta aaaactgtac gctacttaaa aaatggaggg atttacaatg
     5821 gcaactgaaa caaaattaga taacacacag gcagaaaaca aggaaaataa aaatgcggaa
     5881 aacggttcaa aagaaaagaa cggttcaaaa gcaagcaaaa caacaagcag cgggccaatc
     5941 aaacgagcgg tagcaggagg catcatcggt gcaacgattg gatatgtatc gactcctgaa
     6001 aatcgaaaaa gtctccttga ccgcattgat acagacgaat taaaaagcaa agcatctgat
     6061 ttaggaacaa aggtaaaaga aaaatcaaaa agcagcgtgg ccagcctgaa aacatctgcg
     6121 ggaagcttgt ttaaaaaaga taaagataaa tcaaaagatg atgaagaaaa cgtaaattct
     6181 tctagtagcg aaacagaaga cgataacgtt caagagtacg acgagttaaa agaagaaaat
     6241 caaactcttc aagatcgctt atcacagctt gaagaaaaaa tgaacatgct tgttgagctt
     6301 agcctcaata aaaatcaaga cgaagaagcg gaagatacag attccgacga agaagagaac
     6361 gatgagaacg atgaaaacga tgaaaacgag caggacgatg aaaacgaaga agaaacatct
     6421 aagccacgta aaaaggataa aaaagaagct gaggaagaag aaagtgaaag tgacgaagac
     6481 agcgaggaag aagaggaaga ttctcgctca aacaaaaaaa ataaaaaagt aaaaacagaa
     6541 gaagaagacg aagatgaaag cgaagaagaa aaaaaggaag cgaaaccaaa aaagtcaaca
     6601 gctaaaaaat caaaaaatac aaaagcaaag aaaaacacgg acgaagaaga tgatgaagca
     6661 acatctcttt ctagtgaaga cgatacaaca gcctaagacg taaaggagga aagaaagaca
     6721 tgagtacagg cccttctttt tcaactaaag acaatacgct tgaatacttt gtgaaagctt
     6781 ctaataaaca cggcttttca cttgatattt cattaaatgt aaacggcgct gtgatttccg
     6841 gtaccatgat ttcagcaaaa gaatactttg attacttaag cgaaacgttt gaagaaggca
     6901 gtgaagtggc tcaggcgcta agcgaacaat tctctttagc aagcgaagcg agcgaatcaa
     6961 acggagaagc agaagcccat tttattcatt tgaaaaatac aaagatttac tgtggagaca
     7021 gtaaatctac tccttctaaa ggcaaaatct tttggagagg gaaaatagca gaagtagacg
     7081 ggtttttctt aggaaagatt tctgatgcaa aatcaacgag taaaaagagt tcataaaaaa
     7141 cggcggggtg attgccccgc cgttttttag tgatgtgatg agatgtgcag cttctttttt
     7201 cgatacatga gcggagtcat cttcatttct tttcgaaact gattaataaa atagcttgta
     7261 ctattaaacc ctacttgata ggcgacttcc gtcacatttg cttctgcttg ctgcagtccg
     7321 gattcgcgaa cgcgtctcga gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     7381 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     7441 gaggggtttt ttgctgaaag gaggaactat atccgactag tagcccgcct aatgagcggg
     7501 ct
//
