LOCUS       sequence-457070-       11312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral vector for expression of optimized GFP-TEAD
            inhibitor (TEADiv2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Branch B, Yuan Y, Cascone M, Raimondi F, Iglesias-Bartolome R
  TITLE     An improved TEAD dominant-negative protein inhibitor to study Hippo 
            YAP1/TAZ-dependent transcription.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 3:2024.10.03.615022. doi: 
            10.1101/2024.10.03.615022.
  PUBMED    39502361
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 3:2024.10.03.615022. doi: 
            10.1101/2024.10.03.615022."
COMMENT     Sequence Label: pInducer20-EGFP-TEADiv2
FEATURES             Location/Qualifiers
     source          1..11312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2399,2400..2418,2419..2441,
                     2442..2460,2461..2483,2484..2502)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2535..2573
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2570..2594
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2703..2727
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2787..2796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2793..2795,2796..2798,2799..3509)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGVK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2838..2859)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3099..3118)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3446..3467
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3819..3839
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3884..3908)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3961..3987
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4073..5284
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5121..5140
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5343..5960,5961..5966,5967..6002,6003..6005,6006..6041,
                     6042..6044,6045..6080,6081..6089)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6142..6715
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6309..6326)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6536..6555
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6731..7525
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6731..7525
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6785..6804)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7395..7414
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7702..8290
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7755..7775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8173..8184)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8365..8598
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8717..8735)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8803..8907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8908..8976,8977..9768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9126..9145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9939..10527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10428..10447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10681..10698
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10938..10959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10974..10979,10980..10997,10998..11004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11012..11028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11017..11039
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11036..11052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11036..11052
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11102..11121
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt gtttaccact
     2401 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2461 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     2521 ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     2581 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     2641 gggaccgatc cagcctccgc ggccccgaac tagtccagtg tggtggaatt ctgcagatat
     2701 caacaagttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg acggccattg
     2761 tggccatcaa gcttggtacc agatctgcca ccatggtgag caagggcgag gagctgttca
     2821 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2881 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2941 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     3001 agtgcttcag ccgctacccc gaccacatga agcagcacga cttctttaag tccgccatgc
     3061 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3121 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3181 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3241 acgtctatat catggccgac aagcagaaga acggcgtcaa ggtgaacttc aagatccgcc
     3301 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3361 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     3421 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3481 tcactctcgg catggacgag ctgtacaagt ccggactcag atctggatcc tcagtagacg
     3541 accactttgc gaaagcactg ggtgatacat ggctgcaaat aggtgacccc cctgtcgcga
     3601 ccaatcccaa aacggccaat gttccgcaaa cagtgcccat gcggttgcgg aagttgccgg
     3661 agtctttttt caaagagccc gagggagacc ctccagtggc gacgaacccc aaaccctctt
     3721 catggcgaaa aaagattctg cccgagagct tttttaaaga accgggggac ccgccagttg
     3781 cgacaaagcg aacagcggat ggcagtgagt tcgagtcccc gaagaaaaag cgcaaggttg
     3841 agtaagcggc cgcactcgag atatcggcct ccagggcctc tagacccagc tttcttgtac
     3901 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
     3961 tacccatacg acgtcccaga ctacgcttag taatgattaa ttaaactaga aattctaccg
     4021 ggtaggggag gcgcttttcc caaggcagtc tggagtaacc cacccaagat ctggcctccg
     4081 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     4141 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     4201 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     4261 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     4321 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     4381 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     4441 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     4501 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     4561 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     4621 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     4681 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     4741 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     4801 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     4861 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     4921 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     4981 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     5041 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     5101 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     5161 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     5221 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     5281 agacgaagct tggtaccgag ctcggatctc caccccgtac cggtcctgca gtcgaattca
     5341 ccatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     5401 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     5461 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     5521 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     5581 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     5641 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     5701 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     5761 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     5821 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     5881 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     5941 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     6001 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     6061 attttgacct tgacatgctc cccgggtaac taagtaagga tccgcggccg cactagagga
     6121 attccgcccc tctccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag
     6181 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     6241 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     6301 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     6361 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     6421 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     6481 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     6541 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6601 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6661 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagctta
     6721 ccggtccacc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6781 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6841 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6901 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6961 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     7021 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     7081 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     7141 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7201 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     7261 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7321 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     7381 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     7441 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     7501 tcgccttctt gacgagttct tctgatgtac aagtaaagcg gccgcgactc tagatcataa
     7561 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     7621 tgaacctgaa acataaaatg aatgcaattg ttgttgttta gtccctccca attcgatatc
     7681 aagcttatcg cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     7741 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7801 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7861 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7921 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7981 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8041 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8101 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8161 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8221 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8281 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8341 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8401 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8461 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8521 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8581 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     8641 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     8701 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     8761 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8821 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8881 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8941 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9001 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9061 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9121 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     9181 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9241 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9301 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9361 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9421 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9481 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9541 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9601 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9661 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9721 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9781 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9841 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9901 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9961 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10021 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10081 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10141 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10201 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10261 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10321 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10381 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10441 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10501 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10561 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10621 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    10681 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    10741 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
    10801 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10861 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
    10921 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    10981 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    11041 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
    11101 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    11161 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    11221 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    11281 atttgttaaa tatgtactac aaacttagta gt
//
