LOCUS       sequence-457145-       12734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MET CDS without 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Cerqua M, Foiani M, Boccaccio C, Comoglio PM, Altintas DM
  TITLE     The integrated stress response drives MET oncogene overexpression in
            cancers.
  JOURNAL   EMBO J. 2025 Jan 7. doi: 10.1038/s44318-024-00338-4.
  PUBMED    39774381
REFERENCE   2  (bases 1 to 12734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2025 Jan 7. doi: 10.1038/s44318-024-00338-4."
COMMENT     Sequence Label: pLenti-Exon2-MET
FEATURES             Location/Qualifiers
     source          1..12734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     101..118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    235..256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(271..276,277..294,295..301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     314..336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     333..349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     333..349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     368..388
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        370..388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        433..812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        869..1060
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(892..912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1079..1305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1306..1486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1533..1658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2151..2384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2569..2613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2826..2943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3001..3407
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    join(7680..8254,8255..8257,8258..8266)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7847..7864)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8074..8093
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8267..8269,8270..8272,8273..8986)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8267..8269,8270..8272,8273..8983)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8312..8333)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8573..8592)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8920..8941
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9002..9590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9055..9075)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9473..9484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9662..9895
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9973..10094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10010..10029)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10064..10083
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10134..10269
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10289..10308)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10290..10308)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10318..10335)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10318..10334)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10327..10349)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10476..10931
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10563..10582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10773..10794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10957..11061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11062..11130,11131..11922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11280..11299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12093..12681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12582..12601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc
     2821 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2881 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2941 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gctagctgca
     3001 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
     3061 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
     3121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
     3181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
     3241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
     3301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
     3361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cggccggtcg aatcaagctt
     3421 atcgataccg tcgacggatc caccggtact agtatactcc ggaataaacc tctcataatg
     3481 aaggcccccg ctgtgcttgc acctggcatc ctcgtgctcc tgtttacctt ggtgcagagg
     3541 agcaatgggg agtgtaaaga ggcactagca aagtccgaga tgaatgtgaa tatgaagtat
     3601 cagcttccca acttcaccgc ggaaacaccc atccagaatg tcattctaca tgagcatcac
     3661 attttccttg gtgccactaa ctacatttat gttttaaatg aggaagacct tcagaaggtt
     3721 gctgagtaca agactgggcc tgtgctggaa cacccagatt gtttcccatg tcaggactgc
     3781 agcagcaaag ccaatttatc aggaggtgtt tggaaagata acatcaacat ggctctagtt
     3841 gtcgacacct actatgatga tcaactcatt agctgtggca gcgtcaacag agggacctgc
     3901 cagcgacatg tctttcccca caatcatact gctgacatac agtcggaggt tcactgcata
     3961 ttctccccac agatagaaga gcccagccag tgtcctgact gtgtggtgag cgccctggga
     4021 gccaaagtcc tttcatctgt aaaggaccgg ttcatcaact tctttgtagg caataccata
     4081 aattcttctt atttcccaga tcatccattg cattcgatat cagtgagaag gctaaaggaa
     4141 acgaaagatg gttttatgtt tttgacggac cagtcctaca ttgatgtttt acctgagttc
     4201 agagattctt accccattaa gtatgtccat gcctttgaaa gcaacaattt tatttacttc
     4261 ttgacggtcc aaagggaaac tctagatgct cagacttttc acacaagaat aatcaggttc
     4321 tgttccataa actctggatt gcattcctac atggaaatgc ctctggagtg tattctcaca
     4381 gaaaagagaa aaaagagatc cacaaagaag gaagtgttta atatacttca ggctgcgtat
     4441 gtcagcaagc ctggggccca gcttgctaga caaataggag ccagcctgaa tgatgacatt
     4501 cttttcgggg tgttcgcaca aagcaagcca gattctgccg aaccaatgga tcgatctgcc
     4561 atgtgtgcat tccctatcaa atatgtcaac gacttcttca acaagatcgt caacaaaaac
     4621 aatgtgagat gtctccagca tttttacgga cccaatcatg agcactgctt taataggaca
     4681 cttctgagaa attcatcagg ctgtgaagcg cgccgtgatg aatatcgaac agagtttacc
     4741 acagctttgc agcgcgttga cttattcatg ggtcaattca gcgaagtcct cttaacatct
     4801 atatccacct tcattaaagg agacctcacc atagctaatc ttgggacatc agagggtcgc
     4861 ttcatgcagg ttgtggtttc tcgatcagga ccatcaaccc ctcatgtgaa ttttctcctg
     4921 gactcccatc cagtgtctcc agaagtgatt gtggagcata cattaaacca aaatggctac
     4981 acactggtta tcactgggaa gaagatcacg aagatcccat tgaatggctt gggctgcaga
     5041 catttccagt cctgcagtca atgcctctct gccccaccct ttgttcagtg tggctggtgc
     5101 cacgacaaat gtgtgcgatc ggaggaatgc ctgagcggga catggactca acagatctgt
     5161 ctgcctgcaa tctacaaggt tttcccaaat agtgcacccc ttgaaggagg gacaaggctg
     5221 accatatgtg gctgggactt tggatttcgg aggaataata aatttgattt aaagaaaact
     5281 agagttctcc ttggaaatga gagctgcacc ttgactttaa gtgagagcac gatgaataca
     5341 ttgaaatgca cagttggtcc tgccatgaat aagcatttca atatgtccat aattatttca
     5401 aatggccacg ggacaacaca atacagtaca ttctcctatg tggatcctgt aataacaagt
     5461 atttcgccga aatacggtcc tatggctggt ggcactttac ttactttaac tggaaattac
     5521 ctaaacagtg ggaattctag acacatttca attggtggaa aaacatgtac tttaaaaagt
     5581 gtgtcaaaca gtattcttga atgttatacc ccagcccaaa ccatttcaac tgagtttgct
     5641 gttaaattga aaattgactt agccaaccga gagacaagca tcttcagtta ccgtgaagat
     5701 cccattgtct atgaaattca tccaaccaaa tcttttatta gtggtgggag cacaataaca
     5761 ggtgttggga aaaacctgaa ttcagttagt gtcccgagaa tggtcataaa tgtgcatgaa
     5821 gcaggaagga actttacagt ggcatgtcaa catcgctcta attcagagat aatctgttgt
     5881 accactcctt ccctgcaaca gctgaatctg caactccccc tgaaaaccaa agcctttttc
     5941 atgttagatg ggatcctttc caaatacttt gatctcattt atgtacataa tcctgtgttt
     6001 aagccttttg aaaagccagt gatgatctca atgggcaatg aaaatgtact ggaaattaag
     6061 ggaaatgata ttgaccctga agcagttaaa ggtgaagtgt taaaagttgg aaataagagc
     6121 tgtgagaata tacacttaca ttctgaagcc gttttatgca cggtccccaa tgacctgctg
     6181 aaattgaaca gcgagctaaa tatagagtgg aagcaagcaa tttcttcaac cgtccttgga
     6241 aaagtaatag ttcaaccaga tcagaatttc acaggattga ttgctggtgt tgtctcaata
     6301 tcaacagcac tgttattact acttgggttt ttcctgtggc tgaaaaagag aaagcaaatt
     6361 aaagatctgg gcagtgaatt agttcgctac gatgcaagag tacacactcc tcatttggat
     6421 aggcttgtaa gtgcccgaag tgtaagccca actacagaaa tggtttcaaa tgaatctgta
     6481 gactaccgag ctacttttcc agaagatcag tttcctaatt catctcagaa cggttcatgc
     6541 cgacaagtgc agtatcctct gacagacatg tcccccatcc taactagtgg ggactctgat
     6601 atatccagtc cattactgca aaatactgtc cacattgacc tcagtgctct aaatccagag
     6661 ctggtccagg cagtgcagca tgtagtgatt gggcccagta gcctgattgt gcatttcaat
     6721 gaagtcatag gaagagggca ttttggttgt gtatatcatg ggactttgtt ggacaatgat
     6781 ggcaagaaaa ttcactgtgc tgtgaaatcc ttgaacagaa tcactgacat aggagaagtt
     6841 tcccaatttc tgaccgaggg aatcatcatg aaagatttta gtcatcccaa tgtcctctcg
     6901 ctcctgggaa tctgcctgcg aagtgaaggg tctccgctgg tggtcctacc atacatgaaa
     6961 catggagatc ttcgaaattt cattcgaaat gagactcata atccaactgt aaaagatctt
     7021 attggctttg gtcttcaagt agccaaaggc atgaaatatc ttgcaagcaa aaagtttgtc
     7081 cacagagact tggctgcaag aaactgtatg ctggatgaaa aattcacagt caaggttgct
     7141 gattttggtc ttgccagaga catgtatgat aaagaatact atagtgtaca caacaaaaca
     7201 ggtgcaaagc tgccagtgaa gtggatggct ttggaaagtc tgcaaactca aaagtttacc
     7261 accaagtcag atgtgtggtc ctttggcgtg ctcctctggg agctgatgac aagaggagcc
     7321 ccaccttatc ctgatgtaaa cacctttgat ataactgttt acttgttgca agggagaaga
     7381 ctcctacaac ccgaatactg cccagacccc ttatatgaag taatgctaaa atgctggcac
     7441 cctaaagccg aaatgcgccc atccttttct gaactggtgt cccggatatc agcaatcttc
     7501 tctactttca ttggggagca ctatgtccat gtgaacgcta cttatgtgaa cgtaaaatgt
     7561 gtcgctccat atccttctct gttgtcatca gaagataacg ctgatgatga ggtggacaca
     7621 cgaccagcct ccttctggga gacatcatag acgcgtatgc atgagtaact gaggatccgc
     7681 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     7741 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     7801 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     7861 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     7921 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     7981 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     8041 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     8101 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac
     8161 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     8221 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg
     8281 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     8341 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     8401 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     8461 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     8521 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     8581 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     8641 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     8701 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     8761 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     8821 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     8881 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     8941 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtgatcgc gttaagtcga
     9001 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     9061 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     9121 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     9181 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     9241 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     9301 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     9361 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     9421 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     9481 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     9541 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     9601 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     9661 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     9721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     9901 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     9961 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10021 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10081 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
    10141 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    10201 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    10261 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
    10321 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
    10381 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
    10441 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
    10501 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    10561 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    10621 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    10681 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    10741 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    10801 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    10861 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
    10921 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    10981 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    11041 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    11101 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    11161 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    11221 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    11281 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    11341 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    11401 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    11461 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    11521 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    11581 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    11641 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    11701 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    11761 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    11821 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    11881 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    11941 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    12001 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    12061 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    12121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    12181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    12241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    12301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    12361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    12421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    12481 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    12541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    12601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    12661 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    12721 tgctggcctt ttgc
//
