LOCUS       sequence-457150-        8706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 8706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-TEAD1
FEATURES             Location/Qualifiers
     source          1..8706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2161..2187
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2191..2217
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2221..2247
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3538..4112,4113..4115,4116..4124)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3705..3722)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3932..3951
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    4563..5151
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4616..4636)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5034..5045)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5223..5456
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5534..5655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5571..5590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5625..5644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5695..5830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5850..5869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5851..5869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5879..5896)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5879..5895)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5888..5910)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6037..6492
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6124..6143)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6334..6355
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6518..6622
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6623..6691,6692..7483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6841..6860)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7654..8242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8143..8162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8396..8413
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8530..8551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8566..8571,8572..8589,8590..8596)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8604..8620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8609..8631
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8628..8644
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8628..8644
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8663..8683
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8665..8683
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatggg tcaacaggga
     2161 tatccctatg acgtaccaga ttacgccgga tatccttacg acgtgcctga ctatgctggg
     2221 tatccgtacg acgtgcccga ttacgctatt gagcccagca gctggagcgg cagtgagagc
     2281 cctgccgaaa acatggaaag gatgagtgac tctgcagata agccaattga caatgatgca
     2341 gaaggggtct ggagccccga catcgagcaa agctttcagg aggccctggc tatctatcca
     2401 ccatgtggga ggaggaaaat catcttatca gacgaaggca aaatgtatgg taggaatgaa
     2461 ttgatagcca gatacatcaa actcaggaca ggcaagacga ggaccagaaa acaggtgtct
     2521 agtcacattc aggttcttgc cagaaggaaa tctcgtgatt ttcattccaa gctaaaggat
     2581 cagactgcaa aggataaggc cctgcagcac atggcggcca tgtcctcagc ccagatcgtc
     2641 tcggccactg ccattcataa caagctgggg ctgcctggga ttccacgccc gaccttccca
     2701 ggggcgccgg ggttctggcc gggaatgatt caaacagggc agccaggatc ctcacaagac
     2761 gtcaagcctt ttgtgcagca ggcctacccc atccagccag cggtcacagc ccccattcca
     2821 gggtttgagc ctgcatcggc cccagctccc tcagtccctg cctggcaagg tcgctccatt
     2881 ggcacaacca agcttcgcct ggtggaattt tcagcttttc tcgagcagca gcgagaccca
     2941 gactcgtaca acaaacacct cttcgtgcac attgggcatg ccaaccattc ttacagtgac
     3001 ccattgcttg aatcagtgga cattcgtcag atttatgaca aatttcctga aaagaaaggt
     3061 ggcttaaagg aactgtttgg aaagggccct caaaatgcct tcttcctcgt aaaattctgg
     3121 gctgatttaa actgcaatat tcaagatgat gctggggctt tttatggtgt aaccagtcag
     3181 tacgagagtt ctgaaaatat gacagtcacc tgttccacca aagtttgctc ctttgggaag
     3241 caagtagtag aaaaagtaga gacggagtat gcaaggtttg agaatggccg atttgtatac
     3301 cgaataaacc gctccccaat gtgtgaatat atgatcaact tcatccacaa gctcaaacac
     3361 ttaccagaga aatatatgat gaacagtgtt ttggaaaact tcacaatttt attggtggta
     3421 acaaacaggg atacacaaga aactctactc tgcatggcct gtgtgtttga agtttcaaat
     3481 agtgaacacg gagcacaaca tcatatttac aggcttgtaa aggactgagc aattccgccc
     3541 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     3601 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     3661 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3721 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3781 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3841 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3901 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3961 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     4021 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     4081 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatgaag accttcaaca
     4141 tctctcagca ggaccttgag ctggtggaag ttgccactga gaaaatcacc atgctctatg
     4201 aggacaacaa gcaccatgtt ggtgctgcca tcaggaccaa gacaggagaa atcatctctg
     4261 ctgtccacat tgaagcctac attggcaggg tcactgtgtg tgcagaggcc attgccattg
     4321 ggtctgcagt ctccaatggg cagaaggact ttgacaccat tgtggctgtg aggcacccct
     4381 acagtgatga ggtggacagg tccatcagag tggtgtcccc ctgtggcatg tgcagggagc
     4441 tcatctcaga ctatgcccct gattgctttg ttctgattga gatgaatggc aagctggtca
     4501 agacaaccat tgaggagctg attccactga aatacaccag aaactaaacg cgttaagtcg
     4561 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4621 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4681 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4741 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4801 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4861 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4921 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4981 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5041 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5101 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
     5161 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5221 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5281 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5341 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5401 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     5461 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     5521 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5581 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5641 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     5701 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5761 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5821 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     5881 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5941 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6001 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     6061 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6121 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6181 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6241 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6301 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6361 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6421 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6481 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6541 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6601 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6661 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6721 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6781 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6841 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6901 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6961 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7021 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7081 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7141 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7201 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7261 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7321 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7381 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7441 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7501 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7561 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7801 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8041 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8221 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8281 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8341 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8401 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     8461 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     8521 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     8581 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     8641 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     8701 gcaagc
//
