LOCUS       sequence-457153-        9204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 9204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-TAZ-4D
FEATURES             Location/Qualifiers
     source          1..9204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2152..2178
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2182..2208
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3427..4001,4002..4004,4005..4013)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3594..3611)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3821..3840
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4014..5033
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    5061..5649
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5114..5134)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5532..5543)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5721..5954
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6032..6153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6069..6088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6123..6142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6193..6328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6348..6367)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6349..6367)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6377..6394)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6377..6393)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6386..6408)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6535..6990
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6622..6641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6832..6853
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7016..7120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7121..7189,7190..7981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7339..7358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8152..8740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8641..8660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8894..8911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9028..9049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9064..9069,9070..9087,9088..9094)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9102..9118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9107..9129
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9126..9142
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9126..9142
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9161..9181
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9163..9181
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatggc ttacccctac
     2161 gacgttcccg actatgctgg ctacccctat gacgttcctg actacgcggg gagcatgaat
     2221 cccgcttcag cacctccgcc gctgccgccc ccaggtcagc aggtaataca tgtaacacag
     2281 gatctggaca cggacttgga agctttgttt aactccgtca tgaacccgaa gccatcaagc
     2341 tggagaaaga aaatattgcc tgaatctttc ttcaaagaac cagacgacgg tgaccacgac
     2401 cgacaatctt ccactgactc tagtggaggt catcctggac cccgcttggc gggaggcgca
     2461 cagcatgtaa gatcacattc ggaccctgcc agtctccagc ttggcacggg ggccggagct
     2521 gcggggtctc ctgcacaaca gcacgcccat ctgcggcagc aatcttatga tgtcactgat
     2581 gaacttccac tgcccccagg ctgggagatg acgtttaccg cgactgggca gaggtatttt
     2641 ctcaaccata tagagaagat aaccacttgg caagacccaa ggaaggccat gaaccagccc
     2701 ctcaatcaca tgaatctcca cccagctgtt tcttcaactc cagtcccgca gcgcagtatg
     2761 gcagtgagtc aaccgaatct ggttatgaat catcaacatc aacaacaaat ggctcccagt
     2821 acgctctcac aacaaaacca tccgacgcaa aatccccctg ctggactcat gtcaatgccc
     2881 aacgcgctta cgacccaaca acagcagcaa cagaagttga gactgcagag gatccaaatg
     2941 gagagggaaa gaatacggat gcgccaggag gaacttatga gacaagaagc tgcgctttgc
     3001 cgccagcttc ccatggaagc agagaccctg gccccggtac aagcggcggt gaatccccca
     3061 accatgacgc cggacatgag atctatcacc aataactcct ctgacccatt cttgaatgga
     3121 ggtccctacc atagccgaga acagtccacc gattctggtc tgggattggg atgctacagt
     3181 gtcccgacca ccccagaaga tttcctcagc aacgtcgacg aaatggatac tggtgaaaac
     3241 gctggtcaga cccctatgaa catcaaccca cagcagactc gatttccgga cttcctcgac
     3301 tgcttgccgg gcactaacgt cgacttggga acgctcgagt cagaggattt gataccgctc
     3361 ttcaacgacg tagaatccgc gctcaataaa agtgagccat tcctgacgtg gctctgagca
     3421 attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     3481 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     3541 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     3601 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     3661 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     3721 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     3781 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     3841 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     3901 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     3961 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatgaaaa
     4021 agcctgaact caccgctacc tctgtcgaga agtttctgat cgaaaagttc gacagtgtta
     4081 gcgacctgat gcagctctcc gagggcgaag aatctcgggc tttcagcttc gatgtgggag
     4141 ggcgtggata tgtcctgcgg gtgaatagct gcgccgatgg tttctacaaa gatcgctatg
     4201 tttatcggca ctttgcatcc gccgctctcc ctattcccga agtgcttgac attggggagt
     4261 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacc ttgcaagacc
     4321 tgcctgaaac cgaactgccc gctgttctcc agcccgtcgc cgaggccatg gatgccatcg
     4381 ctgccgccga tcttagccag accagcgggt tcggcccatt cggacctcaa ggaatcggtc
     4441 aatacactac atggcgcgat ttcatctgcg ctattgctga tccccatgtg tatcactggc
     4501 aaactgtgat ggacgacacc gtcagtgcct ccgtcgccca ggctctcgat gagctgatgc
     4561 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgccgatttc ggctccaaca
     4621 atgtcctgac cgacaatggc cgcataacag ccgtcattga ctggagcgag gccatgttcg
     4681 gggattccca atacgaggtc gccaacatct tcttctggag gccctggttg gcttgtatgg
     4741 agcagcagac ccgctacttc gagcggaggc atcccgagct tgcaggatct cctcggctcc
     4801 gggcttatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     4861 tcgatgatgc agcttgggct cagggtcgct gcgacgcaat cgtccggtcc ggagccggga
     4921 ctgtcgggcg tacacaaatc gcccgcagaa gcgctgccgt ctggaccgat ggctgtgtgg
     4981 aagtgctcgc cgatagtgga aacagacgcc ccagcactcg tcctagggca aaggatctgc
     5041 agtagacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     5101 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5161 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5221 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5281 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcaactc ctttccggga
     5341 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5401 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5461 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5521 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5581 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5641 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5701 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5761 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5821 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5881 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5941 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     6001 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     6061 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6121 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     6181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6241 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     6301 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     6361 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6421 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6481 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     6541 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6601 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6661 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6721 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6781 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6841 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6901 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6961 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     7021 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7081 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7141 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7201 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7261 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7321 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7381 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7441 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7501 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7561 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7621 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7681 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7741 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7801 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7861 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7921 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7981 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8041 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8101 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8161 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8221 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8281 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8341 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8401 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8461 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8521 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8581 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8641 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8701 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8761 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8821 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8881 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8941 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     9001 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     9061 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9121 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     9181 ggaacaaaag ctggagctgc aagc
//
