LOCUS       sequence-457155-        5041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of a de novo designed, FRET-based 
            neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh 
            AH-W
  TITLE     De novo luciferases enable multiplexed bioluminescence imaging.
  JOURNAL   Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. 
            Epub 2024 Nov 12.
  PUBMED    40417169
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem."; 
            date: "2025-03-13"; volume: "11(3):102346. doi"; pages: " 
            10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12"
COMMENT     Sequence Label: pBAD-luxKate
FEATURES             Location/Qualifiers
     source          1..5041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1384..1401
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1442..1459)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1612..1698
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1790..1817
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1836..1927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1928..1996,1997..2788)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2146..2165)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2959..3547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3448..3467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3701..3718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3733..3873
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3799..3818)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3959..3981
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4137..5015)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gggctcagga acagacgaag aaatagcaga attcgtaaaa
      361 gcattctacg aagcactcga cgcaggagac gcagaaacag cagcagacct actattcgga
      421 gcaggatgca aagaaataca cctatgggac ggaacagtat tcacaagcaa agaaggattc
      481 gaaacatggt tcaaaaaact acaaagcgca gtaacaggag caaaaagaaa aatagaaagc
      541 gtagaaataa aagacggaga agcagtagta aaactaacac taacagcaac atacaaagca
      601 acaggaaaaa aattcgtagt agaactagaa cacacattca cattcgacag agaaagaaac
      661 gaactagtag aagtgaaagt cacagcaaca ccatatatta aagaaaatat gcacatgaaa
      721 ctgtatatgg aaggcacggt gaacaaccac cattttaaat gtaccagcga aggtgaaggc
      781 aaaccgtatg aaggtacgca gaccatgcgc attaaagcgg ttgaaggcgg tccgctgccg
      841 tttgcgtttg atattctggc gaccagcttt atgtatggca gcaaaacctt tattaaccat
      901 acccagggca ttccggattt ctttaaacag agctttccgg aaggttttac ctgggaacgc
      961 gtgaccacgt acgaagatgg tggcgtgctg actgcgactc aagataccag cctgcaggat
     1021 ggctgcctga tttataacgt gaaaattcgc ggtgtgaact ttccgagcaa cggcccggtg
     1081 atgcagaaaa agaccttagg ctgggaagcg agcaccgaaa ccctgtatcc ggcggatggc
     1141 ggtttagaag gccgtgcgga tatggcgctg aaattagtgg gcggcggcca tctgatttgc
     1201 aacctgaaaa ccacttatcg ttcgaaaaag ccggcgaaaa acttaaaaat gccgggcgtg
     1261 tattatgtgg atcgccgtct ggaacgcata aaagaagcgg ataaagaaac ctatgtcgaa
     1321 cagcatgaag tggcggtggc gcgctattgc gatctgccaa gcaaactggg ccatcgcggt
     1381 tctcatcatc atcatcatca ttaaagcttg gctgttttgg cggatgagag aagattttca
     1441 gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg
     1501 gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg
     1561 ccgatggtag tgtggagtaa gcccatgcga gagtagggaa ctgccaggca tcaaataaaa
     1621 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1681 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     1741 gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc
     1801 ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc
     1861 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1921 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1981 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2041 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2101 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2161 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2221 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2281 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2341 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2401 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2461 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2521 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2581 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2641 tggtagtcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2701 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2761 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2821 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2881 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2941 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3001 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3061 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3721 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3781 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     3841 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     3901 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     3961 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc
     4021 aattcgcgcg cgaaggcgaa gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg
     4081 attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat
     4141 gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg
     4201 ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca
     4261 ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg
     4321 atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt
     4381 gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg
     4441 tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga
     4501 tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc
     4561 gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg
     4621 tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt
     4681 gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga
     4741 taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc
     4801 aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg
     4861 cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg
     4921 agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat
     4981 cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga
     5041 g
//
