LOCUS       sequence-457160-        5053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of a de novo designed, FRET-based 
            neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh 
            AH-W
  TITLE     De novo luciferases enable multiplexed bioluminescence imaging.
  JOURNAL   Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. 
            Epub 2024 Nov 12.
  PUBMED    40417169
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem."; 
            date: "2025-03-13"; volume: "11(3):102346. doi"; pages: " 
            10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12"
COMMENT     Sequence Label: pBAD-luxGold
FEATURES             Location/Qualifiers
     source          1..5053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1396..1413
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1454..1471)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1624..1710
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1802..1829
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1848..1939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1940..2008,2009..2800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2158..2177)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2971..3559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3460..3479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3713..3730
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3745..3885
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3811..3830)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3971..3993
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4149..5027)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gggctcagga acagacgaag aaatagcaga attcgtaaaa
      361 gcattctacg aagcactcga cgcaggagac gcagaaacag cagcagacct actattcgga
      421 gcaggatgca aagaaataca cctatgggac ggaacagtat tcacaagcaa agaaggattc
      481 gaaacatggt tcaaaaaact acaaagcgca gtaacaggag caaaaagaaa aatagaaagc
      541 gtagaaataa aagacggaga agcagtagta aaactaacac taacagcaac atacaaagca
      601 acaggaaaaa aattcgtagt agaactagaa cacacattca cattcgacag agaaagaaac
      661 gaactagtag aagtgaaagt cacagcaaca ccatatttta ccggcgtggt gccgattctg
      721 gttgaattgg atggtgatgt gaacggccat aaattttctg tgagcggtga aggggaaggc
      781 gacgcgacct atggtaaact gaccttgaaa tttatttgca ccaccggaaa actgccagtg
      841 ccgtggccga ccctggtgac tagcttgggt tatggtctgc agtgctttgc gcgctatccg
      901 gaccacatga aacagcatga tttctttaaa agcgctatgc cggaaggcta tgtgcaggaa
      961 cgcaccattt tcttcaaaga tgatggtaac tataaaaccc gcgccgaagt aaaatttgaa
     1021 ggtgatacct tagtgaaccg cattgaactg aaaggcattg attttaaaga agatggaaac
     1081 attctgggcc acaaattaga atataactac aacagccata atgtgtatat taccgccgat
     1141 aaacagaaaa atggcattaa agccaatttt aaaatccgcc ataacattga agacggcggc
     1201 gtgcagctgg ccgatcatta tcagcagaac accccgattg gcgatggccc ggtgctgctg
     1261 ccggataacc attatctgag ctatcagagc aaactgagta aagatccgaa cgaaaaacgc
     1321 gatcacatgg tgttactgga gtttgtgacc gcggcgggca ttaccctggg catggatgaa
     1381 ctgtataaag gttctcatca tcatcatcat cattaaagct tggctgtttt ggcggatgag
     1441 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     1501 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     1561 aacgccgtag cgccgatggt agtgtggagt aagcccatgc gagagtaggg aactgccagg
     1621 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1681 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     1741 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
     1801 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt
     1861 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1921 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1981 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2041 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2101 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2161 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     2221 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     2281 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     2341 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     2401 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     2461 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     2521 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     2581 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2641 agccggtgag cgtggtagtc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     2701 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2761 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2821 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2881 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2941 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3001 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3061 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3121 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3181 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3241 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3301 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3361 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3421 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3481 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3541 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3601 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3661 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3721 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3781 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3841 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     3901 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3961 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4021 aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg cataatgtgc ctgtcaaatg
     4081 gacgaagcag ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc
     4141 gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg
     4201 aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg
     4261 tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc
     4321 ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg
     4381 cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata
     4441 tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta
     4501 tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca
     4561 agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt
     4621 tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta
     4681 ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa
     4741 acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct
     4801 ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc
     4861 accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg
     4921 ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca
     4981 ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc
     5041 ccgccattca gag
//
