LOCUS       sequence-457179-       11277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one plasmid for labeling of XIST RNA via Csm complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Xia C, Colognori D, Jiang XS, Xu K, Doudna JA
  TITLE     Single-molecule live-cell RNA imaging with CRISPR-Csm.
  JOURNAL   Nat Biotechnol. 2025 Feb 18. doi: 10.1038/s41587-024-02540-5.
  PUBMED    39966655
REFERENCE   2  (bases 1 to 11277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Feb 18. doi: 10.1038/s41587-024-02540-5."
COMMENT     Sequence Label: pCX419
FEATURES             Location/Qualifiers
     source          1..11277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             268..291
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             298..318
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2602..2649
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2680..2883
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2914..2923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2923..2946
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2953..2973
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3361..3408
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3439..3642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3673..3682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3682..3705
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3712..3732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4402..5118
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYKG"
     CDS             4402..5118
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYKG"
     primer_bind     complement(4429..4457)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5052..5072
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    5119..5166
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5193..5396
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5427..5436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5436..5459
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5466..5486
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6393..6440
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        6471..6674
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      6705..6714
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6714..6737
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6744..6764
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7845..7892
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        7923..8126
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8157..8166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8166..8189
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8196..8216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8955..9002
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        9033..9281
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9033..9053
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9204..9223
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(9437..9455)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9523..9627
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9628..9696,9697..10488)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9846..9865)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10659..11247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11148..11167
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatctctag agccgccacc
      241 atgcaccatc accatcacca ttccggcgat tacaaagacg atgacgataa gatggcgcct
      301 aagaagaaac gcaaagtgcg gggcatgaag aaagaaaaga ttgatctgtt ttacggagcc
      361 ctgctggccg ccatcggaaa ggtcatccag cgagcaaccg gagagcggaa gaaacacgca
      421 cttgtgggcg ccgactggtt cgacgagatc gccgacaacc aagtcatctc ggatcagatc
      481 cggtaccata tggccaacta ccagtctgat aagctcggca acgatcacct ggcttacatc
      541 acctacattg ccgacaacat cgcctccggt gtcgaccgcc ggcaatccaa cgaagagtca
      601 gacgaagata cctccgcaaa gatctgggac acctacacga accaggccga catctttaac
      661 gtgttcggag cgcagaccga taagcggtac ttcaagccta ccgtgctgaa tctcaagtcg
      721 aagcccaact tcgcgtccgc cacttacgaa ccctttagca agggcgatta cgctgccatc
      781 gccacccgga ttaagaacga actggccgag ttcgagttca accaagtcca gattgactcc
      841 ctgctcaacc ttttcgaggc tactctctcc ttcgtgccgt caagcaccaa cactaaggaa
      901 atcgccgaca tctccctggc cgaccattcc cgcttgactg ctgccttcgc tctggcgatc
      961 tacgactacc tggaggacaa gggtcggcac aactacaaag aggacctgtt caccaaagtg
     1021 tcagcgttct atgaagaaga agccttcctg ctggcctcct tcgacctgtc gggaatccag
     1081 gactttatct acaacattaa catcgcaact aacggcgcgg cgaagcagct gaaggcccgg
     1141 agcctctacc tggactttat gtccgagtac atcgccgata gcctgctgga caagctggga
     1201 ctgaacaggg ctaacatgct ttacgtcggc ggcggacacg cctacttcgt cctggccaac
     1261 accgaaaaga ctgtggaaac cctggtgcag tttgagaagg atttcaacca gttcctgttg
     1321 gcaaacttcc agacccgcct ctatgtggcc tttggctggg gttccttcgc ggccaaggac
     1381 atcatgtccg agctgaatag ccccgagtcc taccgccaag tgtaccaaaa ggcttcgcgc
     1441 atgatctcca aaaagaaaat ctccagatac gactaccaga cactgatgct cctgaatcgc
     1501 ggtggaaagt cctcagagag agagtgcgag atttgccact ccgtggagaa cctggtgtcc
     1561 taccacgacc agaaagtctg tgacatttgc cggggactgt accagttctc gaaagaaatt
     1621 gcccatgacc acttcatcat taccgaaaat gaggggctgc cgattggacc aaacgcgtgc
     1681 ttaaagggcg tggcattcga aaagctgtcc caagaagcgt tcagccgggt ctacgtgaag
     1741 aatgactata aggccggtac cgtgaaggct acgcatgtgt tcgtggggga ttaccagtgc
     1801 gacgagatct acaactacgc cgccctgagc aagaacgaga acggcctagg catcaagaga
     1861 ctggccgtgg tccggctcga cgtggatgac ttgggcgccg ccttcatggc cggtttcagc
     1921 cagcagggaa acggacaata ctccactctg tcaagatcgg ccacattctc ccggagcatg
     1981 tcgctgttct tcaaagtgta cattaaccag ttcgcctccg acaagaagct gagcattatc
     2041 tacgcgggcg gcgatgacgt gttcgccatt ggatcgtggc aggatatcat cgcgttcact
     2101 gtggaacttc gcgaaaactt catcaagtgg accaacggga agctcaccct ctccgcgggg
     2161 atagggttgt tcgccgacaa gactcctatt agcctgatgg ctcaccagac cggggaactg
     2221 gaagaggccg ccaagggcaa cgaaaaggac tccatctcgc tgttctcaag cgactacact
     2281 ttcaagtttg ataggttcat cactaacgtg tacgacgaca aactggaaca gattagatac
     2341 ttcttcaacc atcaagacga gaggggaaag aacttcatct ataagcttat tgagcttttg
     2401 aggaaccacg accgcatgaa tatggcacgc ctcgcctatt acctcactcg cctggaagaa
     2461 ctgacccggg agactgacag ggacaagttc aagaccttca agaacctgtt ctactcctgg
     2521 tacaccaaca agaacgataa ggaccggaag gaagccgagc tcgcgctcct gctgtacatc
     2581 tacgaaatca gaaaggatta acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     2641 cgtttgttcg cacacattag ctagccgtca gcacacattg tgatgcggtt ttggcagtac
     2701 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2761 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2821 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2881 gctcgtttag tgaaccgtca gatctctaga gccgccacca tggattacaa agacgatgac
     2941 gataagatgg cgcctaagaa gaaacgcaaa gtgcggggca tgaccatcct gaccgacgag
     3001 aactacgtgg acatcgccga gaaagccatc ctgaagctgg aaagaaacac cagaaataga
     3061 aagaaccctg atgccttctt cctgaccaca tctaagctgc ggaacctgct gagcctgaca
     3121 agcaccctgt tcgacgagag caaggtgaag gaatacgacg ccctgctgga cagaatcgct
     3181 tatctgagag tgcagttcgt gtaccaggcc ggcagagaga tcgccgtgaa agatctgatc
     3241 gagaaggccc agatcctgga agctctgaaa gagatcaagg accgggaaac cctgcagaga
     3301 ttctgcagat acatggaagc cctggtggcc tacttcaagt tctacggcgg caaggactga
     3361 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcag ttcttttgcc
     3421 ttactttcaa tgcatgcggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3481 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3541 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3601 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     3661 atctctagag ccgccaccat ggattacaaa gacgatgacg ataagatggc gcctaagaag
     3721 aaacgcaaag tgcggggcat gaccttcgcc aagatcaaat tcagcgccca gatccggctg
     3781 gaaaccggcc tgcacatcgg aggatctgat gcctttgccg ctatcggcgc catcgccagc
     3841 cctgtgatca aggaccccat caccaacctg cctatcatcc ccggctctag cctgaagggc
     3901 aagatgagaa cactgctggc caaggtgtac aacgaaaagg tggccgagaa gcctagcgac
     3961 gacagcgaca tcctgagcag actgttcgga aatagcaagg ataagcggtt caagatgggc
     4021 agactgatct tccgggacgc cttcctgagc aacgccgacg agctggattc tctgggcgtg
     4081 cggagctaca ccgaggtgaa gttcgagaac accatcgata gaatcaccgc cgaggccaat
     4141 cctagacaga tcgagagagc cattcggaac tcaacattcg acttcgagct gatctacgag
     4201 atcactgatg agaatgagaa ccaggtcgag gaagatttca aggtgatcag agacggcctg
     4261 aagctgctgg aactggacta cctgggcgga agcggctcca gaggctacgg caaagtggct
     4321 tttgagaacc tgaaagccac cacagtgttc ggcaactacg acgtgaaaac cctgaacgag
     4381 ctgctgaccg ccgaagtggg tagcaaagga gaagaacttt tcactggagt tgtcccaatt
     4441 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa
     4501 ggtgatgcta caaacggaaa actcaccctt aaatttattt gcactactgg aaaactacct
     4561 gttccgtggc caacacttgt cactactctg acctatggtg ttcaatgctt ttcccgttat
     4621 ccggatcaca tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     4681 gaacgcacta tatctttcaa agatgacggg acctacaaga cgcgtgctga agtcaagttt
     4741 gaaggtgata cccttgttaa tcgtatcgag ttaaagggta ttgattttaa agaagatgga
     4801 aacattcttg gacacaaact cgagtacaac tttaactcac acaatgtata catcacggca
     4861 gacaaacaaa agaatggaat caaagctaac ttcaaaattc gccacaacgt tgaagatggt
     4921 tccgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     4981 ttaccagaca accattacct gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag
     5041 cgtgaccaca tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat
     5101 gagctctaca aaggttaacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     5161 ttgttcttgc gagaatggac tagtagcaaa ctgtgatgcg gttttggcag tacatcaatg
     5221 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5281 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     5341 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     5401 tagtgaaccg tcagatctct agagccgcca ccatggatta caaagacgat gacgataaga
     5461 tggcgcctaa gaagaaacgc aaagtgcggg gcatgactta caagctctac attatgacct
     5521 ttcaaaacgc ccacttcggt tccggcactc tggactcatc gaagctgacc ttctccgcgg
     5581 atagaatctt ctcggcactc gtgctcgagg ctctgaagat gggaaagctc gacgccttct
     5641 tggccgaggc caaccaggat aagttcactc tgaccgacgc gttcccattc caattcggtc
     5701 ctttcctgcc gaaaccgatt ggttacccca agcacgacca gatcgaccag tctgtggacg
     5761 tgaaggaagt ccgccgccaa gcgaagctgt ccaaaaagct ccagttcctg gctctggaaa
     5821 acgtcgacga ctacctgaac ggagagctgt ttgagaatga ggaacacgcc gtgatcgaca
     5881 cagtgaccaa gaaccagccc cataaagatg ataatctgta ccaagtggcc accactcggt
     5941 tctcgaacga cacctccctt tacgtgatcg ccaacgaatc cgatctgctg aacgaactga
     6001 tgagcagcct tcagtactcc gggctgggcg gcaaaaggtc ctcaggattc ggcagatttg
     6061 agctggacat ccagaacatt cccttggaac tgtccgaccg gctgacgaag aaccacagcg
     6121 acaaggtcat gtcacttacc accgccctcc cggtggacgc tgatctcgag gaagcgatgg
     6181 aagatggcca ttacctgttg accaagtcgt ccggattcgc attctcccac gccaccaacg
     6241 aaaactatcg gaagcaggac ctgtacaagt tcgcctccgg gagcaccttc agcaagactt
     6301 tcgagggaca gatcgtggac gtgcgccctc tcgatttccc tcacgccgtg ctgaactacg
     6361 ccaagccgct gttctttaag ctcgaagtct aacggcaata aaaagacaga ataaaacgca
     6421 cggtgttggg tcgtttgttc gcacattcaa aaacaggcaa ttggacaagc gtgatgcggt
     6481 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     6541 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     6601 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     6661 atataagcag agctcgttta gtgaaccgtc agatctctag agccgccacc atggattaca
     6721 aagacgatga cgataagatg gcgcctaaga agaaacgcaa agtgcggggc atgaaaaatg
     6781 actaccggac cttcaagctg agcctgctga ccctggctcc tatccacatc ggcaacggcg
     6841 agaagtacac cagcagagaa ttcatctacg agaacaagaa gttctacttc cccgacatgg
     6901 gcaagttcta caacaagatg gtggaaaaga gactggccga gaagttcgag gccttcctga
     6961 tccagaccag acccaacgcc agaaacaacc ggctgatttc ttttctgaac gacaacagaa
     7021 tcgccgaaag atcttttggc ggctacagca tcagtgaaac cggcctggaa tctgataaga
     7081 accctaacag cgccggagct atcaacgagg tgaacaaatt catccgggac gccttcggaa
     7141 atccttacat cccaggcagc agcctgaagg gcgccatccg caccatcctg atgaacacca
     7201 cacctaagtg gaacaacgag aacgccgtga acgacttcgg cagattccca aaggaaaaca
     7261 agaacctgat cccttgggga cctaagaaag gcaaggaata cgacgacctg ttcaacgcca
     7321 tcagagtgtc cgacagcaag cccttcgaca acaaaagcct gatcctcgtg cagaagtggg
     7381 actacagcgc caaaaccaac aaggccaagc ctctgcctct gtacagagag tctatcagcc
     7441 ctctgaccaa gatcgagttc gagataacaa caaccactga tgaggccggc agactgatcg
     7501 aggaactggg aaagcgggcc caggcctttt ataaggacta caaggccttt ttcctgtctg
     7561 aattccctga tgataagatc caggctaatc tgcaataccc catctacctg ggcgccggca
     7621 gcggcgcttg gacaaagacc ctgtttaagc aggccgacgg catcctgcag cggagatact
     7681 ccagaatgaa aaccaagatg gtcaagaagg gcgtgctgaa gctgacaaag gcccctctga
     7741 aaacagtgaa gatccccagc ggcaaccaca gcctggtgaa gaatcacgag agcttctacg
     7801 agatgggcaa agccaacttc atgatcaagg aaatcgacaa gtgacggcaa taaaaagaca
     7861 gaataaaacg cacggtgttg ggtcgtttgt tcatgtgtgc cggccggaga ataaaagtct
     7921 aagtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     7981 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     8041 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     8101 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct agagccgcca
     8161 ccatggatta caaagacgat gacgataaga tggcgcctaa gaagaaacgc aaagtgcggg
     8221 gcatgaaaaa gctcgtgttc acctttaagc ggatcgacca ccctgctcag gacctggccg
     8281 tgaaattcca cggcttcctg atggaacagc tggatagcga ctacgtggac tacctgcacc
     8341 agcagcagac caacccctac gccacaaagg tgatccaggg caaagagaac acccagtggg
     8401 tcgtgcatct gctgacagac gacatcgagg acaaggtgtt catgaccctg ctgcagatca
     8461 aggaagtgtc cctgaacgac ctgcctaagt tgtctgtgga aaaggtggaa atccaggagc
     8521 tgggcgctga taagctgctc gagatcttca acagcgagga aaaccagacc tacttcagca
     8581 tcatcttcga gacacctaca ggctttaaaa gccagggcag ctacgtgatc ttccccagca
     8641 tgcggctgat ctttcagagc ctgatgcaga agtacggcag actggtggaa aaccagcctg
     8701 agatcgagga agataccctg gactacctga gcgagcacag caccatcacc aattacagac
     8761 tggaaacaag ctacttcaga gtgcatagac agagaatccc cgccttccgg ggcaagctga
     8821 ccttcaaggt gcagggagcc cagacactga aggcctacgt gaagatgctg ctgaccttcg
     8881 gcgagtacag cggcctgggc atgaaaacca gcctgggaat gggcggcatc aagctggaag
     8941 aaagaaagga ctgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     9001 tcgtgcgata gagggatcca gtgcaccggt tggagggcct atttcccatg attccttcat
     9061 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     9121 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     9181 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     9241 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgatataaac ctaattacct
     9301 cgagagggga cggaaacaaa agcaggtatc cgcggccccg atgggcaaag atataaacct
     9361 aattacctcg agaggggact tttttacatg tgtcagaggt tttcaccgtc atcaccgaaa
     9421 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     9481 atggtttctt agacgtcagg tggcactttt cggggaaatg tgctcggaac ccctatttgt
     9541 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9601 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9661 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9721 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9781 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9841 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     9901 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9961 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    10021 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    10081 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    10141 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    10201 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    10261 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    10321 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    10381 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    10441 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    10501 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10561 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10621 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10681 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10741 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10801 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10861 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10921 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10981 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    11041 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    11101 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    11161 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    11221 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt cttaagc
//
