LOCUS       sequence-457215-        2357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RS421 library variant 14434 in pGLDV_18 (p15A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2357)
  TITLE     Cultivarium plasmids
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGLU_119
FEATURES             Location/Qualifiers
     source          1..2357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(136..152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(136..152)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(149..171)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(332..877)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(412..431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(985..1079)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1100..1759)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1674..1693
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1760..1862)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1949..1971
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1963..1980
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1964..1980
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2282..2300
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 gaccacccca ggatacatag attaccacaa ctccgagccc ttccacctag cgtgctaacc
       61 acgcacgcta ttgttgtata ggctaggtgg aggctcagtg atgataagtc tgcgatggtg
      121 gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca
      181 caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcga
      241 gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac
      301 agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc
      361 atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
      421 aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
      481 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
      541 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
      601 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
      661 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
      721 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
      781 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
      841 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaagtctga
      901 cgctctattc aacaaagccg ccgtccatgg gtagggggct tcaaatcgtc cccccatacg
      961 atataagttg ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1021 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1081 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     1141 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1201 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1261 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1321 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1381 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1441 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1501 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1561 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1621 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1681 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1741 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1801 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     1861 caacgtctca ttgatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac
     1921 tgtgatccac cacaagcgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     1981 catgcataat ccgcacgcat ctggaataag gaagtgccat tccgcctgac cttagcgtgc
     2041 gaacagcacg ctattgttgt atcattactc gcatccattc tcaggctgtc tcgtctcgtc
     2101 tccgaggtct cctcattagt attctctcct taataaatta tttcgttaat gttttcatta
     2161 taaaacaaag gcgattatta gtaaaggtca gctttttaag agttgtttag tttgtgacct
     2221 ctccttaacc tcattcttct atacataaaa gagagaaccg actggttctc tcttgatgtt
     2281 cctgtctctt atacacatct gggtcacgcg taggacgcgt cgagtagggt atgcatgcta
     2341 cccttttgca cgtacga
//
