LOCUS       sequence-457216-       14001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct to express rtTA and SKM proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Ilia K, Shakiba N, Bingham T, Jones RD, Kaminski MM, Aravera E, 
            Bruno S, Palacios S, Weiss R, Collins JJ, Del Vecchio D, Schlaeger 
            TM
  TITLE     Synthetic genetic circuits to uncover the OCT4 trajectories of 
            successful reprogramming of human fibroblasts.
  JOURNAL   Sci Adv. 2023 Dec;9(48):eadg8495. doi: 10.1126/sciadv.adg8495. Epub 
            2023 Nov 29.
  PUBMED    38019912
REFERENCE   2  (bases 1 to 14001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-12"; volume: "9(48):eadg8495. doi"; pages: " 
            10.1126/sciadv.adg8495. Epub 2023 Nov 29"
COMMENT     Sequence Label: lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC
FEATURES             Location/Qualifiers
     source          1..14001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..439
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(98..117)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     308..329
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(448..468)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        453..782
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      633..768
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     695..714
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        830..877
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             896..1270
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1400..1521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1437..1456)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1491..1510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1570..1586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1570..1586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1583..1605)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1594..1610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1618..1624,1625..1642,1643..1648))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1663..1684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1801..1818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1972..2560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2052..2071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2731..3522,3523..3591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3354..3373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3592..3696)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3771..3790)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3962..4341
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4343..4541
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4496..4516
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4559..4739
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4786..4911
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5404..5637
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5822..5866
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6164..6281
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6394..6605
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          6606..7544
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7501..7521
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8208..8228
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8256..8405
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             8427..8483
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9441..9494
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             10998..12314
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="PLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPS
                     EDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTEL
                     LGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNP
                     ARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSL
                     LSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSP
                     SAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRK
                     CTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKAT
                     AYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     misc_feature    12344..12932
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12397..12417)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12815..12826)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13008..13024)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13009..13025)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             13530..13710
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(13736..13753)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13742..13966
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
       61 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      121 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      181 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      241 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      301 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      361 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      421 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
      481 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
      541 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      601 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
      661 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
      721 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
      781 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
      841 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
      901 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
      961 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     1021 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     1081 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     1141 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     1201 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     1261 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1321 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1381 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     1441 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1501 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     1561 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1621 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1681 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1741 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1921 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2221 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2281 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2341 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2401 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2461 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2521 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2581 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2641 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2701 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2761 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2821 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2881 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2941 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3001 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3061 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3121 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3181 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3241 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3301 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3361 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3421 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3481 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3541 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3601 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3661 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3721 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     3781 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     3841 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     3901 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     3961 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     4021 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4081 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4141 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4201 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4261 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4321 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4381 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4441 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4501 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     4561 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4621 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4681 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4741 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     4801 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     4861 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     4921 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     4981 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     5041 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     5101 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     5161 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     5221 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     5281 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     5341 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     5401 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     5461 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     5521 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     5581 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     5641 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     5701 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     5761 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     5821 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     5881 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     5941 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     6001 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     6061 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     6121 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     6181 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     6241 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     6301 gcagagatcc agtttggtta attaacccgt gtcggctcca ggaggtatca caggagaatt
     6361 tcagtacttt aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     6421 ccgagaagtt gtggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     6481 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     6541 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     6601 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     6661 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
     6721 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
     6781 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     6841 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     6901 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     6961 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     7021 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     7081 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     7141 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     7201 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     7261 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     7321 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     7381 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     7441 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     7501 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtggat cccaagggca
     7561 gataagtttg tacaaaaaag agaggtgccg ccaccatgtc tagactggac aagagcaaag
     7621 tcataaactc tgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga
     7681 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     7741 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cactcctgcc
     7801 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg
     7861 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     7921 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     7981 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
     8041 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     8101 ttctgaaaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
     8161 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaccc aagaagaaga
     8221 ggaaggtgga aggaggcggt ggaagcggac gggctgacgc attggacgat tttgatctgg
     8281 atatgctggg aagtgacgcc ctcgatgatt ttgaccttga catgcttggt tcggatgccc
     8341 ttgatgactt tgacctcgac atgctcggca gtgacgccct tgatgatttc gacctggaca
     8401 tgctgattaa ctctagaggc tccggggcca cgaatttctc tctgctgaag caggctggcg
     8461 atgtagagga gaatcctggt ccgtacaaca tgatggaaac cgagctgaag ccgccgggcc
     8521 cgcagcaaac ttcggggggc ggcggcggca actccaccgc ggcggcggcc ggcggcaacc
     8581 agaaaaacag cccggaccgc gtcaagcggc ccatgaatgc cttcatggtg tggtcccgcg
     8641 ggcagcggcg caagatggcc caggagaacc ccaagatgca caactcggag atcagcaagc
     8701 gcctgggcgc cgagtggaaa cttttgtcgg acaccgagaa gcggccgttc atcgacgagg
     8761 ctaagcggct gcgagcgctg cacatgaagg agcacccgga ttataaatac cggccccggc
     8821 ggaaaaccaa gacgctcatg aagaaggata agtacacgct gcccggcggg ctgctggccc
     8881 ccggcggcaa tagcatggcg agcggggtcg gggtgggcgc cggcctgggc gcgggcgtga
     8941 accagcgcat ggacagttac gcgcacatga acggctggag caacggcagc tacagcatga
     9001 tgcaggacca gctgggctac ccgcagcacc cgggcctcaa tgcgcacggc gcagcgcaga
     9061 tgcagcccat gcaccgctac gacgtgagcg ccctgcagta caactccatg accagctcgc
     9121 agacctacat gaacggctcg cccacctaca gcatgtccta ctcgcagcag ggcacccctg
     9181 gcatggctct tggctccatg ggttcggtgg tcaagtccga ggccagctcc agcccccctg
     9241 tggttacctc ttcctcccac tccagggcgc cctgccaggc cggggacctc cgggacatga
     9301 tcagcatgta tctccccggc gccgaggtgc cggaacccgc cgcccccagc agacttcaca
     9361 tgtcccagca ctaccagagc ggcccggtgc ccggcacggc cattaacggc acactgcccc
     9421 tctcacacat gggatctggg gaaggcaggg gctcacttct tacttgtgga gatgttgagg
     9481 agaatccggg tccgaggcag ccacctggcg agtctgacat ggctgtcagc gacgcgctgc
     9541 tcccatcttt ctccacgttc gcgtctggcc cggcgggaag ggagaagaca ctgcgtcaag
     9601 caggtgcccc gaataaccgc tggcgggagg agctctccca catgaagcga cttcccccag
     9661 tgcttcccgg ccgcccctat gacctggcgg cggcgaccgt ggccacagac ctggagagcg
     9721 gcggagccgg tgcggcttgc ggcggtagca acctggcgcc cctacctcgg agagacacgg
     9781 aggagttcaa cgatctcctg gacctggact ttattctctc caattcgctg acccatcctc
     9841 cggagtcagt ggccgccacc gtgtcctcgt cagcgtcagc ctcctcttcg tcgtcgccgt
     9901 cgagcagcgg ccctgccagc gcgccctcca cctgcagctt cacctatccg atccgggccg
     9961 ggaacgaccc gggcgtggcg ccgggcggca cgggcggagg cctcctctat ggcagggagt
    10021 ccgctccccc tccgacggct cccttcaacc tggcggacat caacgacgtg agcccctcgg
    10081 gcggcttcgt ggccgagctc ctgcggccag aattggaccc ggtgtacatt ccgccgcagc
    10141 agccgcagcc gccaggtggc gggctgatgg gcaagttcgt gctgaaggcg tcgctgagcg
    10201 cccctggcag cgagtacggc agcccgtcgg tcatcagcgt cagcaaaggc agccctgacg
    10261 gcagccaccc ggtggtggtg gcgccctaca acggcgggcc gccgcgcacg tgccccaaga
    10321 tcaagcagga ggccgtgtct tcgtgcaccc acttgggcgc tggaccccct ctcagcaatg
    10381 gccaccggcc ggctgcacac gacttccccc tggggcggca gctccccagc aggactaccc
    10441 cgaccctggg tcttgaggaa gtgctgagca gcagggactg tcaccctgcc ctgccgcttc
    10501 ctcccggctt ccatccccac ccggggccca attacccatc cttcctgccc gatcagatgc
    10561 agccgcaagt cccgccgctc cattaccaag agctcatgcc acccggttcc tgcatgccag
    10621 aggagcccaa gccaaagagg ggaagacgat cgtggccccg gaaaaggacc gccacccaca
    10681 cttgtgatta cgcgggctgc ggcaaaacct acacaaagag ttcccatctc aaggcacacc
    10741 tgcgaaccca cacaggtgag aaaccttacc actgtgactg ggacggctgt ggatggaaat
    10801 tcgcccgctc agatgaactg accaggcact accgtaaaca cacggggcac cgcccgttcc
    10861 agtgccaaaa atgcgaccga gcattttcca ggtcggacca cctcgcctta cacatgaaga
    10921 ggcattttgg ttccggtcaa tgcacaaatt acgctttgtt gaagctggcc ggtgatgtgg
    10981 aggaaaatcc cggacccccc ctcaacgtta gcttcaccaa caggaactat gacctcgact
    11041 acgactcggt gcagccgtat ttctactgcg acgaggagga gaacttctac cagcagcagc
    11101 agcagagcga gctgcagccc ccggcgccca gcgaggatat ctggaagaaa ttcgagctgc
    11161 tgcccacccc gcccctgtcc cctagccgcc gctccgggct ctgctcgccc tcctacgttg
    11221 cggtcacacc cttctccctt cggggagaca acgacggcgg tggcgggagc ttctccacgg
    11281 ccgaccagct ggagatggtg accgagctgc tgggaggaga catggtgaac cagagtttca
    11341 tctgcgaccc ggacgacgaa acgttcatca aaaacatcat catccaggac tgtatgtgga
    11401 gcggcttctc ggccgccgcc aagctggtgt cagagaagct ggcctcctac caggctgcgc
    11461 gcaaagacag cggcagcccg aaccccgccc gcggccacag cgtctgctcc acctccagct
    11521 tgtacctgca ggatctgagc gccgccgcct cagagtgcat cgacccctcg gtggtcttcc
    11581 cctaccctct caacgacagc agctcgccca agtcctgcgc ctcgcaagac tccagcgcct
    11641 tctctccgtc ctcggattct ctgctctcct cgacggagtc ctccccgcag ggcagccccg
    11701 agcccctggt gctccatgag gagacaccgc ccaccaccag cagcgactct gaggaggaac
    11761 aagaagatga ggaagaaatc gatgttgttt ctgtggaaaa gaggcaggct cctggcaaaa
    11821 ggtcagagtc tggatcacct tctgctggag gccacagcaa acctcctcac agcccactgg
    11881 tcctcaagag gtgccatgtg tccacacatc agcacaacta cgcagcgcct ccctccactc
    11941 ggaaggacta tcctgctgcc aagagggtca agttggacag tgtcagagtc ctgagacaga
    12001 tcagcaacaa ccgaaaatgc accagcccca ggtcctcgga caccgaggag aatgtcaaga
    12061 ggcgaacaca caacgtcttg gagcgccaga ggaggaacga gctaaaacgg agcttttttg
    12121 ccctgcgtga ccagatcccg gagttggaaa acaatgaaaa ggcccccaag gtagttatcc
    12181 ttaaaaaagc cacagcatac atcctgtccg tccaagcaga ggagcaaaag ctcatttctg
    12241 aagaggactt gttgcggaaa cgacgagaac agttgaaaca caaacttgaa cagctacgga
    12301 actcttgtgc gtaaggcttc cactggattg tacaattacc aacaatcaac ctctggatta
    12361 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
    12421 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
    12481 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
    12541 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
    12601 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
    12661 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    12721 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
    12781 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    12841 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
    12901 gagtcggatc tccctttggg ccgcctcccc gcctgtttcg cctcggcgtc cggtccgtgt
    12961 tgcttggtct tcacctgtgc agacttgcga accatggatt ccgctatcga taccgtcgac
    13021 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
    13081 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
    13141 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    13201 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
    13261 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
    13321 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
    13381 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
    13441 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
    13501 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
    13561 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    13621 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    13681 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
    13741 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
    13801 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
    13861 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
    13921 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
    13981 agaaccagct ggggctctag g
//
