LOCUS       sequence-457217-       11054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen 
            fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Ilia K, Shakiba N, Bingham T, Jones RD, Kaminski MM, Aravera E, 
            Bruno S, Palacios S, Weiss R, Collins JJ, Del Vecchio D, Schlaeger 
            TM
  TITLE     Synthetic genetic circuits to uncover the OCT4 trajectories of 
            successful reprogramming of human fibroblasts.
  JOURNAL   Sci Adv. 2023 Dec;9(48):eadg8495. doi: 10.1126/sciadv.adg8495. Epub 
            2023 Nov 29.
  PUBMED    38019912
REFERENCE   2  (bases 1 to 11054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-12"; volume: "9(48):eadg8495. doi"; pages: " 
            10.1126/sciadv.adg8495. Epub 2023 Nov 29"
COMMENT     Sequence Label: lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE
FEATURES             Location/Qualifiers
     source          1..11054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..30)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15..31)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             536..716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(742..759)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    748..972
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1018..1446
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1105..1124)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1315..1336
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1545..1564)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1556..2365
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1623..1642)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(2441..2458)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2612..3200)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2692..2711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3371..4162,4163..4231))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3994..4013
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4232..4336)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4411..4430)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4602..4981
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4983..5181
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             5199..5379
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5426..5551
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6044..6277
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6462..6506
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6804..6921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7342..7590
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7342..7362
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7513..7532
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     ncRNA           7662..7676
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(7753..7777)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7815..7833
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7840..7858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7865..7883
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7897..7915
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7922..7940
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7947..7965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7979..7997
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8004..8022
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8029..8047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8061..8079
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8086..8104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8111..8129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        8156..8200
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     regulatory      8248..8257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9586..9595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    10070..10658
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10123..10143)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10541..10552)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgcttgagac gatcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
       61 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
      121 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
      181 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
      241 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
      301 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
      361 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
      421 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
      481 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
      541 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      601 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      661 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
      721 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
      781 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
      841 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
      901 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
      961 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     1021 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     1081 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     1141 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     1201 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     1261 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     1321 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     1381 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     1441 cgaattaatt tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     1501 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     1561 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     1621 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     1681 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     1741 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     1801 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     1861 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     1921 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     1981 tcgcgtattt cgactagctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2041 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2101 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     2161 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     2221 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     2281 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     2341 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     2401 cattacgctg acttgacggg acggcgcaag ctcatgcttc ctcgctcact gactcgctgc
     2461 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2521 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2581 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2641 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2701 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2761 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2821 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2881 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2941 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3001 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3061 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3121 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3181 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3241 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3301 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3361 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3421 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3481 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3541 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3601 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3661 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3721 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3781 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3841 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3901 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3961 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4021 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4081 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4141 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4201 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4261 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4321 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     4381 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4441 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     4501 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     4561 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     4621 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     4681 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     4741 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     4801 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     4861 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     4921 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     4981 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     5041 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     5101 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     5161 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     5221 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5281 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5341 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     5401 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     5461 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     5521 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     5581 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     5641 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     5701 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     5761 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     5821 agacaccaag gaagctttag acaagataga ggaggaacaa aacaaaagta agaccaccgc
     5881 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     5941 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     6001 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     6061 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     6121 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     6181 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     6241 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     6301 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     6361 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     6421 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     6481 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     6541 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     6601 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     6661 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     6721 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
     6781 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     6841 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     6901 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
     6961 attaacccgt gtcggctcca cgtctcagga gtcttttgca atgctctttg ggaaattata
     7021 ttagcctaat tactaatttc ctggccctca aggtgacttg cttgaacttg ccaccttcgc
     7081 cactaggggg cagcattggt ttacacaggg tgaacaaacg ttcaattcta aactaatatt
     7141 ctttggtggg aaagtgtgtt cattttggtt tgttttgaag aagtcacttc atacctttga
     7201 actggggtta agctggttaa ctcccaaaat tccaccagtt ccaatatcct atggaaaaac
     7261 cccaaaacca cttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
     7321 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
     7381 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
     7441 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     7501 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
     7561 ttatatatct tgtggaaagg acgaaacacc ggtgctcgct tcggcagcac atatactaca
     7621 ccctaatgat actctcttgt ggaggaagca gacaacaatt agtgaagcca cagatgtaat
     7681 tgttgtcagc ttcctccaca agcgactatc ccgtcgcttc tgcaaagcgg acttcggtcc
     7741 gcttttttct agacccagct ttcttgtaca aagttggcat tatacttaag tttgtacaaa
     7801 aaagaggctt gtattcccta tcagtgatag agaaacgcct ccctatcagt gatagagaaa
     7861 agcttcccta tcagtgatag agaagaagag gtgtattccc tatcagtgat agagaaacgc
     7921 ctccctatca gtgatagaga aaagcttccc tatcagtgat agagaagaag tgaggtattc
     7981 cctatcagtg atagagaaac gcctccctat cagtgataga gaaaagcttc cctatcagtg
     8041 atagagaaga agcctagtat tccctatcag tgatagagaa acgcctccct atcagtgata
     8101 gagaaaagct tccctatcag tgatagagaa gaaggtacca acaaaatgtc gtaacaaggg
     8161 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgcagtgaac cgtcagatcc
     8221 agataagttt gtacaaaaaa gagaggtgcc accatggcag ggcatctggc ctccgacttc
     8281 gctttcagtc cgccgcccgg tggtggggga gatggcccag gcggcccgga acccgggtgg
     8341 gtggatccca gaacctggct gagctttcaa gggcccccgg gcggacctgg catcgggccg
     8401 ggcgttggac ctggttccga ggtttggggt attccgccat gtcctccacc ctatgaattc
     8461 tgtggtggca tggcatactg cgggccgcaa gtaggtgtcg gtctggttcc tcaaggcggt
     8521 ttggaaacta gccaaccaga gggagaagcg ggagtcggag tcgaatctaa ctcagatgga
     8581 gcctctccag aaccgtgtac tgtaacacca ggcgccgtta aacttgagaa ggagaagctg
     8641 gagcaaaatc cggaagaatc tcaggacata aaggcgctcc aaaaagagct tgaacagttc
     8701 gccaaacttc tgaaacagaa acggattact ctgggataca cacaggctga tgtaggcctc
     8761 accctcggtg ttttgttcgg taaggtgttt tctcagacca ccatatgtcg attcgaggct
     8821 cttcagcttt cttttaaaaa catgtgcaag ctgaggccat tgcttcaaaa atgggttgaa
     8881 gaggcagaca acaacgaaaa cctgcaagaa atttgtaaag cagaaaccct cgtccaggcg
     8941 cgcaaacgca aacgcacatc aattgagaac cgagttcgcg ggaacctgga aaacctcttt
     9001 ctccagtgcc ctaaaccaac acttcagcaa atttcccaca tagcgcagca actcgggttg
     9061 gagaaagatg ttgtgcgagt atggttttgc aacagaagac agaaggggaa acggagctcc
     9121 agtgattacg ctcaacggga ggattttgaa gctgccggtt cacctttctc cgggggaccg
     9181 gtttccttcc cgttggctcc cggtcctcac tttggaacac ctggttacgg ctccccgcac
     9241 ttcaccgctc tgtatagctc cgtcccgttc cccgaaggcg aggcttttcc tccagtttcc
     9301 gtcactacat tggggagccc gatgcatagc aatgtgtcca agggcgaaga ggacaacatg
     9361 gccagcctgc ctgccaccca cgagctgcac atcttcggca gcatcaacgg cgtggacttc
     9421 gacatggtgg gacagggcac cggcaacccc aacgacggat acgaggaact gaacctgaag
     9481 tccacaaagg gcgacctgca gttcagcccc tggatcctgg tgccccacat cggctacggc
     9541 ttccaccagt acctgcccta ccctgacggc atgagccctt tccaggccgc catggtggat
     9601 ggctctggct accaggtgca cagaaccatg cagttcgagg acggcgccag cctgaccgtg
     9661 aactacagat acacctacga gggcagccac atcaagggcg aggcccaagt gaagggcaca
     9721 ggcttccctg ctgacggccc cgtgatgacc aactctctga cagccgccga ctggtgcaga
     9781 agcaagaaaa cctaccctaa cgacaagacc atcatcagca ccttcaagtg gtcctacacc
     9841 acaggcaacg gcaagagata cagaagcacc gccagaacca cctacacctt cgccaagccc
     9901 atggccgcca actacctgaa gaaccagcct atgtacgtgt tccgcaagac cgagctgaag
     9961 cactccaaga cagaactgaa cttcaaagag tggcagaaag ccttcaccga cgtgatgggc
    10021 atggacgagc tgtacaagtg agcttccact ggattgtaca attaccaaca atcaacctct
    10081 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
    10141 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
    10201 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
    10261 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
    10321 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
    10381 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
    10441 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
    10501 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
    10561 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
    10621 tcagacgagt cggatctccc tttgggccgc ctccccgcct gtttcgcctc ggcgtccggt
    10681 ccgtgttgct tggtcttcac ctgtgcagac ttgcgaacca tggattcgcc acctttggtc
    10741 ttgacattca ctaaggagtg ggcctggaat aaaaccagaa atccccattc tcagtctgcc
    10801 ccaagccctc caattccgag ggcgctgtat gtataagctc gggcaggcaa aagtcgactg
    10861 tgagaacacg ccagcagagg gcgctgtggc cccatcagtc cctgcccact gaactctcca
    10921 gaaggaaaag cggcaaggat gcaaacaaga aaatcagacc agactgggag tctagaccct
    10981 gcgtcccacg cggtcccacc atggcttcct ctttggaatt ttcaggggga cccaggaaga
    11041 ctgacaccgc tgat
//
