LOCUS       sequence-457257-       11654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a N-terminal truncated version of SREBP1 lacking part of the
            transcriptional activation domain and fused to a tandem of 
            mNeongreen to visualize the import of SREBP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11654)
  AUTHORS   Vercruysse T, Vanstreels E, Jacquemyn M, Boland S, Kilonda A, 
            Allasia S, Vandecaetsbeek I, Klaassen H, Versele M, Chaltin P, 
            Marchand A, Daelemans D
  TITLE     Ibetazol, a novel inhibitor of importin beta1-mediated nuclear 
            import.
  JOURNAL   Commun Biol. 2024 Nov 23;7(1):1560. doi: 10.1038/s42003-024-07237-8.
  PUBMED    39580542
REFERENCE   2  (bases 1 to 11654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-11-23"; volume: "7(1):1560. doi"; pages: " 
            10.1038/s42003-024-07237-8"
COMMENT     Sequence Label: pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..11654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        439..1621
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          670..1612
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1569..1589
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1623..1647
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1728..2432
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      1980..1989
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2445..3146
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEEL
                     NLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGA
                     SLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTF
                     KWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKA
                     FTDVMGMDELYK"
     regulatory      2694..2703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6525..6581
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(7195..7212)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7201..7425
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7521..7949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7608..7627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7818..7839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7958..7978)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7963..8292
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8143..8278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8205..8224
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8359..9153
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8413..8432)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9023..9042
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9327..9448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9364..9383)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9418..9437
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9497..9513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9497..9513)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9510..9532)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9521..9537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9545..9551,9552..9569,9570..9575))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9590..9611
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9728..9745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9899..10487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9979..9998)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10658..11449,11450..11518))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11519..11623)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgccagtacc
      241 caaaaagcgg ggggtcccct ccaccccaca gtggggccac taacgcgagt aattcataca
      301 aaaggactcg cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta
      361 gaacccagag atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt
      421 ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
      481 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
      541 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
      601 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
      661 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
      721 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga
      781 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
      841 tcgtgcttga gttgaggcct ggcttgggcg ctggggccgc cgcgtgcgaa tctggtggca
      901 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
      961 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     1021 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     1081 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     1141 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     1201 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     1261 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     1321 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1381 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     1441 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     1501 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     1561 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     1621 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     1681 catagaagac accgggaccg atccagcctc cgcttaagct tggtaccatg gtgagcaagg
     1741 gcgaggagga taacgcctct ctcccagcga cacatgagtt acacatcttt ggctccatca
     1801 acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg
     1861 agttaaacct gaagtccacc aagggtgacc tccagttctc cccctggatt ctggtccctc
     1921 atatcgggta tggcttccat cagtacctgc cctaccctga cgggatgtcg cctttccagg
     1981 ccgccatggt agatggcagc ggataccaag tccatcgcac aatgcagttt gaagatggtg
     2041 cctcccttac tgttaactac cgctacacct acgagggaag ccacatcaaa ggagaggccc
     2101 aggtgaaggg gactggtttc cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg
     2161 cggactggtg caggtcgaag aagacttacc ccaacgacaa aaccatcatc agtaccttta
     2221 agtggagtta caccactgga aatggcaagc gctaccggag cactgcgcgg accacctaca
     2281 cctttgccaa gccaatggcg gctaactatc tgaagaacca gccgatgtac gtgttccgta
     2341 agacggagct caagcactcc aagaccgagc tcaacttcaa ggagtggcaa aaggccttta
     2401 ccgatgtgat gggcatggac gagctgtaca agggttctgg ctcagtgagc aagggcgagg
     2461 aggataacgc ctctctccca gcgacacatg agttacacat ctttggctcc atcaacggtg
     2521 tggactttga catggtgggt cagggcaccg gcaatccaaa tgatggttat gaggagttaa
     2581 acctgaagtc caccaagggt gacctccagt tctccccctg gattctggtc cctcatatcg
     2641 ggtatggctt ccatcagtac ctgccctacc ctgacgggat gtcgcctttc caggccgcca
     2701 tggtagatgg cagcggatac caagtccatc gcacaatgca gtttgaagat ggtgcctccc
     2761 ttactgttaa ctaccgctac acctacgagg gaagccacat caaaggagag gcccaggtga
     2821 aggggactgg tttccctgct gacggtcctg tgatgaccaa ctcgctgacc gctgcggact
     2881 ggtgcaggtc gaagaagact taccccaacg acaaaaccat catcagtacc tttaagtgga
     2941 gttacaccac tggaaatggc aagcgctacc ggagcactgc gcggaccacc tacacctttg
     3001 ccaagccaat ggcggctaac tatctgaaga accagccgat gtacgtgttc cgtaagacgg
     3061 agctcaagca ctccaagacc gagctcaact tcaaggagtg gcaaaaggcc tttaccgatg
     3121 tgatgggcat ggacgagctg tacaagggca gcggaagtat ggaagacatg cttcagctta
     3181 tcaacaacca agacagtgac ttccctggcc tatttgaccc accctatgct gggagtgggg
     3241 cagggggcac agaccctgcc agccccgata ccagctcccc aggcagcttg tctccacctc
     3301 ctgccacatt gagctcctct cttgaagcct tcctgagcgg gccgcaggca gcgccctcac
     3361 ccctgtcccc tccccagcct gcacccactc cattgaagat gtacccgtcc atgcccgctt
     3421 tctcccctgg gcctggtatc aaggaagagt cagtgccact gagcatcctg cagaccccca
     3481 ccccacagcc cctgccaggg gccctcctgc cacagagctt cccagcccca gccccaccgc
     3541 agttcagctc cacccctgtg ttaggctacc ccagccctcc gggaggcttc tctacaggaa
     3601 gccctcccgg gaacacccag cagccgctgc ctggcctgcc actggcttcc ccaccagggg
     3661 tcccgcccgt ctccttgcac acccaggtcc agagtgtggt cccccagcag ctactgacag
     3721 tcacagctgc ccccacggca gcccctgtaa cgaccactgt gacctcgcag atccagcagg
     3781 tcccggtcct gctgcagccc cacttcatca aggcagactc gctgcttctg acagccatga
     3841 agacggacgg agccactgtg aaggcggcag gtctcagtcc cctggtctct ggcaccactg
     3901 tgcagacagg gcctttgccg accctggtga gtggcggaac catcttggca acagtcccac
     3961 tggtcgtaga tgcggagaag ctgcctatca accggctcgc agctggcagc aaggccccgg
     4021 cctctgccca gagccgtgga gagaagcgca cagcccacaa cgccattgag aagcgctacc
     4081 gctcctccat caatgacaaa atcattgagc tcaaggatct ggtggtgggc actgaggcaa
     4141 agctgaataa atctgctgtc ttgcgcaagg ccatcgacta cattcgcttt ctgcaacaca
     4201 gcaaccagaa actcaagcag gagaacctaa gtctgcgcac tgctgtccac aaaagcaaat
     4261 ctctgaagga tctggtgtcg gcctgtggca gtggagggaa cacagacgtg ctcatggagg
     4321 gcgtgaagac tgaggtggag gacacactga ccccaccccc ctcggatgct ggctcacctt
     4381 tccagagcag ccccttgtcc cttggcagca ggggcagtgg cagcggtggc agtggcagtg
     4441 actcggagcc tgacagccca gtctttgagg acagcaaggc aaagccagag cagcggccgt
     4501 ctctgcacag ccggggcatg ctggaccgct cccgcctggc cctgtgtact ctagtatttc
     4561 tatgtttatc ttgtaaccca ttagcaagtt tgctcggagc tagaggattg ccatcacctt
     4621 cagatacaac ttcagtttat catagtccag gaaggaatgt actcggaaca gagagtcgag
     4681 atggaccagg atgggcgcaa tggctgttgc cacctgtagt ttggctgttg aacggattgc
     4741 tagtattggt ttcactcgta ttgcttttcg tctacggtga acctgtaaca cgacctcata
     4801 gcggacctgc agtctatttc tggcgacaca gaaaacaagc agatctagat cttgctagag
     4861 gtgactttgc acaggctgca caacagctat ggcttgcctt gcgagctcta ggaaggccac
     4921 tgcctacgtc acatcttgat ctggcctgtt ccttgctctg gaatctgatt aggcacctac
     4981 tacaacgtct ctgggtcgga agatggctag ctggtcgtgc aggtggtctg cagcaagact
     5041 gtgctctccg agtagacgct agcgcatccg cacgcgacgc agctttggtt tatcacaaac
     5101 tacatcaact gcatacaatg gggaagcaca cgggaggaca tctcactgct acgaacctgg
     5161 cactctctgc actcaatctt gcagaatgtg ctggagatgc tgtatcagta gctacacttg
     5221 ctgaaatcta tgtagctgct gcattgcgcg tcaaaacctc actccctcga gctttgcatt
     5281 tcctgacacg ctttttcttg agcagtgcaa gacaagcatg tttggctcaa agtggaagtg
     5341 tacctccagc aatgcaatgg ttatgtcatc ctgttggaca tcgcttcttc gtcgatggtg
     5401 attggtcagt actatctact ccatgggaga gtctgtactc tctggcggga aatcctgtcg
     5461 atcctctagc tcaagtcaca caactttttc gagaacacct actggagcga gcactgaact
     5521 gtgtaactca acctaaccca agcccaggga gtgctgatgg agataaagaa ttttcagacg
     5581 cactaggtta tctccagctt ttgaatagtt gctcagatgc agcaggtgcg ccagcttact
     5641 ccttctctat ctcatcttcc atggctacaa ctaccggtgt agatcctgta gctaagtggt
     5701 gggcttcttt gactgctgtt gtaatacatt ggctgagaag agatgaagaa gctgcagaac
     5761 gactttgtcc tctcgtagaa catctacctc gcgttctcca agaaagtgaa cgaccactac
     5821 ctcgagctgc tcttcatagt tttaaggctg cacgtgcatt gttgggttgt gcaaaagcag
     5881 agtcaggacc tgcctctttg actatctgtg agaaagcttc aggttactta caagatagtc
     5941 ttgctacgac acctgcttct agtagcattg ataaagctgt gcaactgttc ctttgcgacc
     6001 ttctactagt agttagaacg tctttgtggc gacagcaaca gccacctgca cctgctcctg
     6061 ctgctcaagg tacctcttct cgtccacaag ccagtgcact ggaattgcga ggttttcaga
     6121 gagatctgtc atctttgaga agattggcac aatcatttag acctgctatg aggcgcgttt
     6181 ttctgcatga agcaacggca cgtctcatgg ctggtgcatc acccactcgt actcaccaat
     6241 tgctcgacag atccttaaga cgacgagcag gtccaggagg taaaggtgga gcagttgctg
     6301 aattagaacc aagaccaacg cgacgagaac atgctgaagc tctgctccta gcaagttgtt
     6361 atcttcctcc aggatttttg tctgcacctg gtcaaagggt aggaatgctt gcagaagcag
     6421 caagaactct tgagaaatta ggtgatcgac gattgctaca cgattgtcaa caaatgctca
     6481 tgcggcttgg tggtggaacg acggtaacct ccagcggaag cggagctact aacttcagcc
     6541 tgctgaagca ggctggagac gtggaggaga accctggacc aatgaccgag tacaagccta
     6601 ctgtcaggtt ggctacaaga gacgacgttc ctagagccgt gagaactctg gctgcagcct
     6661 tcgccgacta ccccgccacg agacacaccg ttgacccaga tcggcatatt gagagagtga
     6721 ctgaactgca ggagctgttt cttacaagag ttggcctcga cataggcaag gtgtgggtgg
     6781 cggacgacgg cgccgccgtg gccgtctgga ccactcccga atcagttgag gctggcgccg
     6841 tattcgctga gatcggcccg agaatggctg agctcagcgg gagtaggctc gcggcacagc
     6901 agcaaatgga gggactgctg gcaccacaca ggcccaaaga acccgcctgg ttcctggcaa
     6961 ccgtcggtgt atctcccgat catcagggga aaggtctggg ctctgccgta gtgctccctg
     7021 gcgtggaggc agctgagaga gcaggagtac ctgccttctt ggagacctcc gctccaagga
     7081 atcttccctt ctatgaacgg ttgggcttca ccgtgacagc cgacgtggaa gtccccgaag
     7141 gcccccgcac ttggtgcatg acgaggaagc ctggagcgtg aacccgctga tcagcctcga
     7201 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7261 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7321 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7381 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttcg ggacaggatt
     7441 ggtgacagaa aagccccccg ctttttgggt actggcttct gaggcggaaa gaaccagctg
     7501 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7561 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7621 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7681 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7741 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7801 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7861 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7921 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     7981 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     8041 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8101 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     8161 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8221 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8281 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     8341 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     8401 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     8461 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     8521 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     8581 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     8641 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     8701 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     8761 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     8821 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     8881 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     8941 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     9001 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     9061 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     9121 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     9181 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     9241 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     9301 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     9361 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9421 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     9481 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9541 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9601 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9661 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9721 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    10021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10321 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10501 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10561 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10621 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10681 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10741 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10801 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10861 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10921 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10981 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11041 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11101 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11161 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11221 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11281 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11341 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11401 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11461 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11521 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11581 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11641 tgccacctga cgtc
//
