LOCUS       sequence-457265-        3458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for 2xHA LTB4R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Pedro MP, Lund K, Kang SWS, Chen T, Stuelten CH, Porat-Shliom N, 
            Iglesias-Bartolome R
  TITLE     GPCR Screening Reveals that the Metabolite Receptor HCAR3 Regulates 
            Epithelial Proliferation, Migration, and Cellular Respiration.
  JOURNAL   J Invest Dermatol. 2024 Jun;144(6):1311-1321.e7. doi: 
            10.1016/j.jid.2023.12.002. Epub 2023 Dec 14.
  PUBMED    38103827
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.12.002"; journalName: "J Invest Dermatol"; date:
            "2024-06"; volume: "144"; issue: "6"; pages: "1311-1321.e7"
COMMENT     Sequence Label: pENTR1A 2xHA LTB4R
FEATURES             Location/Qualifiers
     source          1..3458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             512..538
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             542..568
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(1687..1786)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1898..1917)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1909..2718
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1976..1995)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2808..3396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3297..3316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccggt accgctagcg ccgccaccat gtacccatac gatgttcctg actatgcggg
      541 ctatccctat gacgtcccgg actatgcagg atccggagat ccacctgtcg caaccatgaa
      601 cacgactagc agcgcggcgc ctcctagcct cggtgttgag ttcatatccc tgcttgctat
      661 aatcctgctt tcagtagctt tggccgtggg gctgccaggc aatagctttg ttgtttggtc
      721 cattctcaag agaatgcaaa aacgcagcgt tacggcactt atggtactta atctcgccct
      781 cgccgacctg gctgtcctcc tcactgcgcc ctttttcctt cacttccttg cccaaggcac
      841 atggagtttc ggacttgcag ggtgccgact ctgccactat gtttgcggcg tctcaatgta
      901 cgcttccgta cttcttatta ctgcgatgag cttggataga agtttggccg tggcaaggcc
      961 gtttgtgtca caaaagctcc gcaccaaagc catggctaga agagtattgg ccggcatttg
     1021 ggtattgtca tttcttctcg ctacgcccgt gcttgcatac cgaacagtcg tcccctggaa
     1081 aactaatatg tctttgtgct tcccgcgata tccgagcgag ggtcatagag cttttcattt
     1141 gatatttgag gcggtcacgg gtttcctttt gccctttctc gcggtggtcg catcctactc
     1201 cgatattggt agaaggcttc aggcaagaag attccggcgg tcccgccgca ctggcagatt
     1261 ggtcgtgctg attatactta ctttcgcggc attctggttg ccttatcacg tggtgaacct
     1321 cgcagaggcc gggcgggcat tggccggcca ggcagcaggc ttgggtttgg tcgggaagag
     1381 gctgagtctt gctcgcaatg tgctgattgc tttggcattt cttagcagca gcgtcaatcc
     1441 cgtcttgtac gcttgtgcgg gcggtggact tctgcggtca gccggcgttg gatttgtggc
     1501 aaaactcctc gaaggaaccg gttccgaggc atcaagcacg aggagagggg ggtctttggg
     1561 tcaaaccgca cgctccggcc cggcagcact cgaacctgga ccctctgaaa gcttgactgc
     1621 gagcagtcct cttaagctga atgaactgaa ttagtaagaa ttcgcggccg cactcgagat
     1681 atctagaccc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     1741 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgcag
     1801 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1861 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     1921 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     1981 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     2041 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2101 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2161 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     2221 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2281 tccctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     2341 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     2401 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     2461 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     2521 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     2581 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     2641 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     2701 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacattattc agattgggcc
     2761 ccgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2821 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2881 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2941 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3001 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3061 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3421 cggttcctgg ccttttgctg gccttttgct cacatgtt
//
