LOCUS       sequence-457270-        3644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for GPR137.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Pedro MP, Lund K, Kang SWS, Chen T, Stuelten CH, Porat-Shliom N, 
            Iglesias-Bartolome R
  TITLE     GPCR Screening Reveals that the Metabolite Receptor HCAR3 Regulates 
            Epithelial Proliferation, Migration, and Cellular Respiration.
  JOURNAL   J Invest Dermatol. 2024 Jun;144(6):1311-1321.e7. doi: 
            10.1016/j.jid.2023.12.002. Epub 2023 Dec 14.
  PUBMED    38103827
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.12.002"; journalName: "J Invest Dermatol"; date:
            "2024-06"; volume: "144"; issue: "6"; pages: "1311-1321.e7"
COMMENT     Sequence Label: pENTR1A 2xHA  GPR137
FEATURES             Location/Qualifiers
     source          1..3644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             566..592
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             596..622
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(1873..1972)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2084..2103)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2095..2904
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2162..2181)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2994..3582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3483..3502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccggt accgctagcg ccgccaccac cttcgatcaa ctcgcgccgc tggtcaataa
      541 tggtaccgct agcgccgcca ccatgtaccc atacgatgtt cctgactatg cgggctatcc
      601 ctatgacgtc ccggactatg caggatccgg agatccacct gtcgcaacca tggagtctaa
      661 tctgtcaggg ttggtccctg cagccgggtt ggtgccagcg ctcccaccag cggtaacact
      721 gggattgact gctgcttaca ctactcttta cgctctcctt ttcttttccg tatacgcaca
      781 attgtggttg gtcttgctct acgggcacaa gcgactgagc taccagacag tgttcctcgc
      841 cttgtgtctg ctgtgggcgg cgttgaggac aactcttttc tctttttatt tcagagatac
      901 gccaagagct aatcggctcg gccctcttcc attctggctt ctctactgtt gcccagtgtg
      961 tttgcaattc tttactctga ctttgatgaa tctctacttt gctcaggttg ttttcaaagc
     1021 gaaagtcaaa cgcagacccg agatgtctcg aggattgctt gccgtccgag gtgcttttgt
     1081 cggggcatct ctcctgttcc ttctcgtaaa tgtgctgtgt gctgtgctga gtcaccgacg
     1141 gcgagcacag ccgtgggcgc tgttgcttgt ccgcgtcttg gtgagcgaca gtttgtttgt
     1201 aatctgtgcc ctttcactcg cagcatgcct ctgtctcgtc gctcggcgag caccaagtac
     1261 aagcatctat ttggaagcga aggggaccag tgtgtgccaa gccgctgcta tgggcggcgc
     1321 catggttttg ttgtacgcta gtagggcgtg ctataacttg acagcacttg ccttggcccc
     1381 gcaaagtagg ctggacactt tcgactacga ctggtacaac gtaagcgatc aagcggacct
     1441 cgtaaacgat cttggaaata aagggtatct cgtgttcggt ctgatcctct tcgtctggga
     1501 actgttgccc actaccctgc ttgttggatt tttcagggtc catagaccac cccaggatct
     1561 ctcaacgtca catatactga acggccaagt ttttgcttct agatcctact tcttcgatag
     1621 agcgggacac tgtgaggacg aaggctgctc atgggaacac agtaggggcg agtcaacgag
     1681 tatgagtggt tctctcgggt caggatcatg gtatggtgca atagggaggg aacccggatg
     1741 gtacggaggt agtcaaacga agactacgcc cctgctcttc agtcaggtgc ctgggcccgg
     1801 gggacaccac cacagcctgt actctacccc gcagacctga taagaattcg cggccgcact
     1861 cgagatatct agacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat
     1921 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     1981 ctgcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2041 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     2101 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2161 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     2221 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2281 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2341 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2401 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2461 gcagtgtccc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2521 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2581 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     2641 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     2701 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2761 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2821 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2881 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ttattcagat
     2941 tgggccccgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3001 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3061 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3121 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3181 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3241 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3301 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3361 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3421 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3541 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3601 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
