LOCUS       sequence-457331-        7615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full length mouse FoxP3 (R337Q, A372S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7615)
  TITLE     Hur lab plasmids 2024
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MSCV-FoxP3-R337Q/A372S-IRES-Thy1.1
FEATURES             Location/Qualifiers
     source          1..7615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    467..483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(491..497,498..515,516..521))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    536..557
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(674..691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(845..1433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(925..944)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1604..2395,2396..2464))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2227..2246
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2465..2569)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2637..2655
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2693..2715)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2815..2834
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3028..3050
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3178..3197
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             3397..3913
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    3977..4318
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             4385..4801
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4731..4753
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4769..4785
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4820..4846
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6188..6212)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6246..6812
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6262..6814
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6406..6423)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6633..6652
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6862..7350
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     LTR             join(7406..7615,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
       61 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
      121 tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga
      181 ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
      241 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
      301 ttcatgggta acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat
      361 taagtaatcc tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa
      421 tagttcatta tggacagcgc agaaagagct ggggagaatt gtgaaattgt tatccgctca
      481 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      601 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      661 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1261 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1501 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1561 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1621 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1681 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1741 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1801 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1861 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1921 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1981 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2041 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2101 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2161 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2221 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2281 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2341 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2401 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2461 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2521 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2581 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     2641 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     2701 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     2761 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     2821 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     2881 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     2941 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     3001 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggcgcaag
     3061 gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
     3121 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     3181 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     3241 gcgtccggcg tagaggcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc
     3301 tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa
     3361 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     3421 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata
     3481 gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat
     3541 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     3601 cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     3661 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     3721 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc
     3781 cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
     3841 gaatcgtgga ctcgctgatc cttgggaggg tctcctcaga ttgattgact gcccacctcg
     3901 ggggtctttc atttggaggt tccaccgaga tttggagacc cctgcccagg gaccaccgac
     3961 ccccccgccg ggaggtaagc tggccagcgg tcgtttcgtg tctgtctctg tctttgtgcg
     4021 tgtttgtgcc ggcatctaat gtttgcgcct gcgtctgtac tagttagcta actagctctg
     4081 tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag
     4141 acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg
     4201 gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc
     4261 gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca
     4321 gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     4381 ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     4441 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     4501 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     4561 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     4621 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     4681 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     4741 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     4801 cggaattaga tctatggcgt acccatacga tgttccagat tacgctggat ctcccaaccc
     4861 taggccagcc aagcctatgg ctccttcctt ggcccttggc ccatccccag gagtcttgcc
     4921 aagctggaag actgcaccca agggctcaga acttctaggg accaggggct ctgggggacc
     4981 cttccaaggt cgggacctgc gaagtggggc ccacacctct tcttccttga accccctgcc
     5041 accatcccag ctgcagctgc ctacagtgcc cctagtcatg gtggcaccgt ctggggcccg
     5101 actaggtccc tcaccccacc tacaggccct tctccaggac agaccacact tcatgcatca
     5161 gctctccact gtggatgccc atgcccagac ccctgtgctc caagtgcgtc cactggacaa
     5221 cccagccatg atcagcctcc caccaccttc tgctgccact ggggtcttct ccctcaaggc
     5281 ccggcctggc ctgccacctg ggatcaatgt ggccagtctg gaatgggtgt ccagggagcc
     5341 agctctactc tgcaccttcc cacgctcggg tacacccagg aaagacagca accttttggc
     5401 tgcaccccaa ggatcctacc cactgctggc aaatggagtc tgcaagtggc ctggttgtga
     5461 gaaggtcttc gaggagccag aagagtttct caagcactgc caagcagatc atctcctgga
     5521 tgagaaaggc aaggcccagt gcctcctcca gagagaagtg gtgcagtctc tggagcagca
     5581 gctggagctg gaaaaggaga agctgggagc tatgcaggcc cacctggctg ggaagatggc
     5641 gctggccaag gctccatctg tggcctcaat ggacaagagc tcttgctgca tcgtagccac
     5701 cagtactcag ggcagtgtgc tcccggcctg gtctgctcct cgggaggctc cagacggcgg
     5761 cctgtttgca gtgcggaggc acctctgggg aagccatggc aatagttcct tcccagagtt
     5821 cttccacaac atggactact tcaagtacca caatatgcaa ccccctttca cctatgccac
     5881 ccttatccga tgggccatcc tggaagcccc ggagaggcag aggacactca atgaaatcta
     5941 ccattggttt actcgcatgt tctcctactt cagaaaccac cccgccacct ggaagaatgc
     6001 catccgccac aacctgagcc tgcacaagtg ctttgtgcga gtggagagcg agaagggagc
     6061 agtgtggacc gtagatgaat ttgagtttcg caagaagagg agccaacgcc ccaacaagtg
     6121 ctccaatccc tgcccttgag tcgactggat ccggtaccga attcgcggcc gcactcgaga
     6181 tatctagacc cagctttctt gtacaaagtg gttcgatgac ggtatcgata agcttgatat
     6241 cgaattccgc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     6301 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     6361 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     6421 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     6481 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     6541 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     6601 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     6661 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     6721 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     6781 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggg tcttttctgc
     6841 agtcaccgtc ctcgaggcac catgaaccca gccatcagcg tcgctctcct gctctcagtc
     6901 ttgcaggtgt cccgagggca gaaggtgacc agcctgacag cctgcctggt gaaccaaaac
     6961 cttcgcctgg actgccgcca tgagaataac accaaggata actccatcca gcatgagttc
     7021 agcctgaccc gagagaagag gaagcacgtg ctctcaggca cccttgggat acccgagcac
     7081 acgtaccgct cccgcgtcac cctctccaac cagccctata tcaaggtcct taccctagcc
     7141 aacttcacca ccaaggatga gggcgactac ttttgtgagc ttcgcgtctc gggcgcgaat
     7201 cccatgagct ccaataaaag tatcagtgtg tatagagaca agctggtcaa gtgtggcggc
     7261 ataagcctgc tggttcagaa cacatcctgg atgctgctgc tgctgctttc cctctccctc
     7321 ctccaagccc tggacttcat ttctctgtga cgcgtagaat tcgataaaat aaaagatttt
     7381 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     7441 ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga gaagttcaga
     7501 tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca
     7561 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccc
//
